| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 538,801 | T→C | 10.8% | intergenic (+110/+524) | rhlE → / ← dps | RNA helicase/Fe‑binding and storage protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 538,801 | 0 | T | C | 10.8% | 199.8 / 12.3 | 74 | intergenic (+110/+524) | rhlE/dps | RNA helicase/Fe‑binding and storage protein |
| Reads supporting (aligned to +/- strand): ref base T (34/32); new base C (5/3); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGT > minE/538731‑538861 | cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCAt > 1:1691513/1‑71 (MQ=255) gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCttt < 1:5315014/71‑1 (MQ=255) cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt < 1:264633/71‑1 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTCTg > 1:1009757/1‑71 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTATTg > 1:549139/1‑71 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCttt < 1:4702484/68‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTg > 1:706270/1‑70 (MQ=255) aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTg > 1:1098510/1‑69 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt > 1:5410900/1‑67 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg > 1:2288712/1‑71 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg < 1:622238/71‑1 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg > 1:1438223/1‑71 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg > 1:3133039/1‑71 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg < 1:3098963/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg > 1:2099705/1‑71 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg < 1:2286866/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg < 1:4185033/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg > 1:4025865/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCgg > 1:2246883/1‑69 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa > 1:885521/1‑70 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa > 1:1793241/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGAt < 1:4681069/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGAt < 1:1446537/70‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATaa < 1:263348/71‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGc < 1:3115061/62‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAgg > 1:3476652/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAg < 1:2630273/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTa > 1:1738902/1‑57 (MQ=255) cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg > 1:5196309/1‑62 (MQ=255) cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt > 1:3790145/1‑51 (MQ=255) gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:4961625/69‑1 (MQ=255) aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgcc > 1:169200/1‑71 (MQ=255) gCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc < 1:1674553/71‑1 (MQ=255) cTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg > 1:3667419/1‑71 (MQ=255) cTACGTGAACTCTGCAATATATTGAATTTGAATGCTTTTGTAGGCAGGATaa > 1:1594758/1‑52 (MQ=14) tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:425733/1‑59 (MQ=255) cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg < 1:4783723/69‑1 (MQ=255) cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg < 1:1464454/69‑1 (MQ=255) cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg < 1:942636/69‑1 (MQ=255) gTGAACTCTGCAATATATTGAATTTGCATGCTTTTg < 1:1207570/36‑1 (MQ=255) gTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt < 1:1088517/71‑1 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt > 1:4655884/1‑36 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt > 1:892597/1‑36 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt > 1:899254/1‑36 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt > 1:873523/1‑71 (MQ=255) gAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGATTACGCCGCATCCGGCAttt > 1:46906/1‑71 (MQ=255) aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:4354799/70‑1 (MQ=255) aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:2994025/70‑1 (MQ=255) aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:5217807/70‑1 (MQ=255) aCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:3631997/69‑1 (MQ=255) ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa > 1:5240586/1‑40 (MQ=40) tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac > 1:2133937/1‑70 (MQ=255) tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:3995064/38‑1 (MQ=14) tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:5491886/38‑1 (MQ=14) tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt < 1:1410310/46‑1 (MQ=11) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt > 1:827221/1‑36 (MQ=14) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa > 1:3643886/1‑38 (MQ=14) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:5063152/1‑46 (MQ=11) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:3314255/1‑46 (MQ=11) aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg < 1:955801/71‑1 (MQ=255) aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg < 1:4574589/71‑1 (MQ=255) atatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:4451630/42‑1 (MQ=38) atatTGAATTTGGATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:5085013/1‑42 (MQ=255) atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:4561344/1‑42 (MQ=255) atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAc > 1:5510004/1‑71 (MQ=255) tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:281175/39‑1 (MQ=40) tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAc < 1:4569740/70‑1 (MQ=255) tatTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:3238293/1‑41 (MQ=255) ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:3471444/37‑1 (MQ=40) ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:1659299/37‑1 (MQ=40) ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:1652674/37‑1 (MQ=40) ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:1866687/1‑39 (MQ=255) ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACtt < 1:121407/70‑1 (MQ=255) gAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGt > 1:2533837/1‑70 (MQ=255) gCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac > 1:708396/1‑51 (MQ=255) | CAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGT > minE/538731‑538861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |