| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 558,960 | A→G | 12.2% | Q69Q (CAA→CAG) | cmr → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 558,960 | 0 | A | G | 12.2% | 99.7 / 5.8 | 41 | Q69Q (CAA→CAG) | cmr | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (17/19); new base G (2/3); total (19/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
ATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCGGTGATGCTGGCGGGAG > minE/558891‑559018 | atatCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAAt < 1:3480977/71‑1 (MQ=255) tatCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATg < 1:770611/71‑1 (MQ=255) tatCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATg < 1:5421174/71‑1 (MQ=255) tCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAAt > 1:3938526/1‑68 (MQ=255) gattgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCt > 1:3735237/1‑71 (MQ=255) gattgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCt > 1:3099087/1‑71 (MQ=255) gattgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCt > 1:1613794/1‑71 (MQ=255) attgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCt < 1:5664165/70‑1 (MQ=255) ttgGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGt > 1:3592948/1‑71 (MQ=255) gggTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGc > 1:2431449/1‑66 (MQ=255) ggTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCg > 1:4834167/1‑70 (MQ=255) gTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGa < 1:5145216/71‑1 (MQ=255) tCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTgg > 1:4855454/1‑56 (MQ=255) tCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTgg > 1:5493626/1‑56 (MQ=255) cTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGTATCGt < 1:2509527/71‑1 (MQ=255) cTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCg < 1:3370055/70‑1 (MQ=255) cTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCg < 1:4622537/70‑1 (MQ=255) cTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGt < 1:1168012/71‑1 (MQ=255) cGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTa > 1:2171554/1‑66 (MQ=255) cGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTg < 1:2587475/69‑1 (MQ=255) cGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTg < 1:4481084/69‑1 (MQ=255) cGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGt > 1:648688/1‑71 (MQ=255) cGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGt > 1:983826/1‑71 (MQ=255) aCCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGcc < 1:4582602/70‑1 (MQ=255) cgcgTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTc > 1:2157784/1‑71 (MQ=255) tctATCTTGCTGGCGGTATGGCGTTACAGTGGCTGCTGGGGCCGCTTTCCGACAGAATTGGCCGCAGGCCg > 1:1792202/3‑71 (MQ=255) cgTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTcc < 1:2524799/70‑1 (MQ=255) gTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCgg < 1:3187092/71‑1 (MQ=255) gTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCgg < 1:5559143/71‑1 (MQ=255) tCTTGCTGGCGGTATGGCGTTACAGTGGCTGCTGGGGCCGCTTTCCGACAGAATTGGCCg < 1:4474671/60‑1 (MQ=255) tCTTGCTGGCGGTATGGCGTTACAGTGGCTGCTGGGGCCGCTTTCCGACAGAATTGGCCGCAGGCCgg < 1:3590787/68‑1 (MQ=255) tCTTGCTGGCGGTATGGCGTTACAGTGGCTGCTGGGGCCGCTTTCCGACAGAATTGGCCGCAGGCCgg < 1:2676914/68‑1 (MQ=255) gcgggATGTTTTTACAATGGCTGCTGGGGCCGCTGTCgg < 1:5157700/39‑1 (MQ=255) gATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCGGTGATGCTGGCGGGAg < 1:4317998/71‑1 (MQ=255) gTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCg > 1:3151194/1‑45 (MQ=255) gTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCg > 1:367359/1‑45 (MQ=255) tttttACAATGGCTGCTGGGGCCGCTGTCGGATCg < 1:2850000/35‑1 (MQ=255) tttttACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTcc < 1:1351681/50‑1 (MQ=255) tttACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCGGTg > 1:2286765/1‑52 (MQ=255) tttACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCGGTGa > 1:776816/1‑53 (MQ=255) aCAGTGGCTGCTGGGGCCGCTTTCCGACAGAATTGGCCGCAGGCCg > 1:3574839/1‑46 (MQ=255) | ATATCAGGCGGGCATTGATTGGGTTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTGCTGGGGCCGCTGTCGGATCGTATTGGTCGCCGTCCGGTGATGCTGGCGGGAG > minE/558891‑559018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |