Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,028 C→T 9.5% S742S (TCG→TCA) ‡ pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,0280CT 9.5% 261.8 / 10.8 94S742S (TCG→TCA) ‡pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (55/29);  new base T (4/5);  total (59/35)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCG  >  minE/607959‑608098
                                                                      |                                                                      
aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGc                                                                        <  1:2762980/70‑1 (MQ=255)
aGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGc                                                                        <  1:2413541/70‑1 (MQ=255)
    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGtt                                                                   >  1:973397/1‑71 (MQ=255)
    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGtt                                                                   >  1:4352840/1‑71 (MQ=255)
    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGtt                                                                   >  1:2591745/1‑71 (MQ=255)
    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGtt                                                                   <  1:645509/71‑1 (MQ=255)
    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGtt                                                                   >  1:229397/1‑71 (MQ=255)
    aaaTCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGtt                                                                   <  1:2151974/71‑1 (MQ=255)
       tCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGa                                                                      <  1:1309261/65‑1 (MQ=255)
        cTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGaaa                                                               >  1:982748/1‑71 (MQ=255)
        cTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGaaa                                                               >  1:4477486/1‑71 (MQ=255)
        cTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGaaa                                                               >  1:2170650/1‑71 (MQ=255)
           aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGaaa                                                               <  1:4419698/68‑1 (MQ=255)
           aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACg                                                             >  1:2298041/1‑70 (MQ=255)
           aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACg                                                             <  1:1962975/70‑1 (MQ=255)
           aTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGt                                                            <  1:3026259/71‑1 (MQ=255)
            ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGt                                                            >  1:4396549/1‑70 (MQ=255)
            ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTa                                                           >  1:2300988/1‑71 (MQ=255)
            ttACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGATGCT‑GTTCTTTAGTCAGCGAGTTGAAACGt                                                            >  1:3494523/1‑70 (MQ=255)
               cATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACt                                                         <  1:4815956/70‑1 (MQ=255)
                 tAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGGCAGCGAGTTGAAACGTACTGc                                                       <  1:3749529/70‑1 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:2244981/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:3197352/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:210154/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:2338224/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:176854/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:2561674/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGt                                                                    >  1:71531/1‑56 (MQ=255)
                  aGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCg                                                      >  1:3536577/1‑70 (MQ=255)
                     ttGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGc                                                  <  1:2617571/71‑1 (MQ=255)
                      tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACg                                                             >  1:1953076/1‑59 (MQ=255)
                      tGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACg                                                             >  1:1801633/1‑59 (MQ=255)
                       gAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGAGAGTTGAAACGTACTGCGTAGcc                                                 <  1:83408/70‑1 (MQ=255)
                         gtaaaGGTACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGa                                              <  1:350587/68‑1 (MQ=255)
                          tGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGt                                                            <  1:1294776/56‑1 (MQ=255)
                            aaGGTACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGaga                                            <  1:2969174/70‑3 (MQ=255)
                            aaGGTACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGaga                                            <  1:404675/70‑3 (MQ=255)
                            aaGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                            >  1:227011/1‑70 (MQ=255)
                            aaGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                            >  1:5315911/1‑70 (MQ=255)
                             aGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACg                                                             >  1:805418/1‑52 (MQ=255)
                             aGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACg                                                             >  1:5010636/1‑52 (MQ=255)
                                tACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                         >  1:3279435/1‑69 (MQ=255)
                                tACGTGAAATAACATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACg                                        >  1:398507/1‑70 (MQ=255)
                                    aGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACTGATgg                                   <  1:87811/71‑1 (MQ=255)
                                    aGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATgg                                   <  1:3814349/71‑1 (MQ=255)
                                      taataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                       <  1:3326983/65‑1 (MQ=255)
                                      taataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                       <  1:5158368/65‑1 (MQ=255)
                                      taataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACg                                        <  1:366473/64‑1 (MQ=255)
                                       aataaCATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGCATTGTCa                                <  1:569736/71‑1 (MQ=255)
                                        ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                     >  1:2715948/1‑65 (MQ=255)
                                        ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                     >  1:531529/1‑65 (MQ=255)
                                        ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                     >  1:5538430/1‑65 (MQ=255)
                                        ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGAt                                     >  1:3593189/1‑65 (MQ=255)
                                        ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                               <  1:472346/71‑1 (MQ=255)
                                        ataaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                               >  1:1064665/1‑71 (MQ=255)
                                        ataaCATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                               >  1:4040379/1‑71 (MQ=255)
                                         taaCATCCTGTTGCT‑GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                <  1:3450180/69‑1 (MQ=255)
                                          aaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTa                                                           >  1:2849456/1‑41 (MQ=255)
                                          aaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTa                                                           >  1:3235411/1‑41 (MQ=255)
                                          aaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTa                                                           >  1:739180/1‑41 (MQ=255)
                                          aaCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAg                                                   <  1:1961296/49‑1 (MQ=255)
                                           aCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGt                                                     >  1:1115134/1‑46 (MQ=255)
                                           aCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACgg                                       >  1:2975706/1‑60 (MQ=255)
                                           aCGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                >  1:2340301/1‑67 (MQ=255)
                                             gTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                          >  1:3563115/1‑71 (MQ=255)
                                             gTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                          >  1:5527442/1‑71 (MQ=255)
                                              tCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                  <  1:5225139/62‑1 (MQ=255)
                                               ccTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTAc                                                          >  1:2986568/1‑37 (MQ=255)
                                                ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                >  1:753132/1‑62 (MQ=255)
                                                ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                        >  1:2966344/1‑70 (MQ=255)
                                                ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                        >  1:1178530/1‑70 (MQ=255)
                                                ctgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                        >  1:3375138/1‑70 (MQ=255)
                                                ctgctgca‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                        >  1:562899/1‑70 (MQ=255)
                                                 tgctgct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                             <  1:1232043/64‑1 (MQ=255)
                                                   ctgctgGTTCTTTAGTCAGCGAGTTGAAACGTACTGc                                                       >  1:4867945/1‑37 (MQ=38)
                                                      ct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGcc                                                 <  1:1052531/39‑1 (MQ=255)
                                                      ct‑gTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                 <  1:1119205/71‑1 (MQ=255)
                                                          ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc               <  1:1488244/70‑1 (MQ=255)
                                                           tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg             >  1:3291109/1‑71 (MQ=255)
                                                           tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg             >  1:2144533/1‑71 (MQ=255)
                                                           tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg             >  1:5385737/1‑71 (MQ=255)
                                                            cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                              >  1:5045808/1‑37 (MQ=255)
                                                            cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGa                                              >  1:4501310/1‑37 (MQ=255)
                                                            cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                            >  1:2000940/1‑39 (MQ=255)
                                                            cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATa                                            >  1:2851405/1‑39 (MQ=255)
                                                            cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                               >  1:4205704/1‑52 (MQ=255)
                                                                aGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                 <  1:5332446/62‑1 (MQ=255)
                                                                 gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg             >  1:3904211/1‑65 (MQ=9)
                                                                 gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc       >  1:4713787/1‑71 (MQ=255)
                                                                 gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc       <  1:2381112/71‑1 (MQ=255)
                                                                  tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc      <  1:4341197/71‑1 (MQ=255)
                                                                  tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc      <  1:1067665/71‑1 (MQ=255)
                                                                    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt          <  1:5478999/65‑1 (MQ=255)
                                                                    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt          <  1:862411/65‑1 (MQ=255)
                                                                    aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt    >  1:4465790/1‑71 (MQ=255)
                                                                     gCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                  <  1:2426693/40‑1 (MQ=255)
                                                                      cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcg  >  1:2599153/1‑71 (MQ=255)
                                                                      |                                                                      
AGTCAAATCTAATTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTGCT‑GTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCG  >  minE/607959‑608098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑