Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,064	G→T	27.5%	T730T (ACC→ACA)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,064	0	G	T	27.5%	152.6 / 37.5	69	T730T (ACC→ACA)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base T (8/11);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

AGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAA  >  minE/607995‑608131                                                                      |                                                                    aGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACTGATgg                                                                    <  1:87811/71‑1 (MQ=255) aGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATgg                                                                    <  1:3814349/71‑1 (MQ=255)    aataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGCATTGTCa                                                                 <  1:569736/71‑1 (MQ=255)     ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                                                <  1:472346/71‑1 (MQ=255)     ataaCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                                                >  1:1064665/1‑71 (MQ=255)     ataaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                                                >  1:4040379/1‑71 (MQ=255)      taaCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                                 <  1:3450180/69‑1 (MQ=255)        aCGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                                                 >  1:2340301/1‑67 (MQ=255)          gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                                           >  1:3563115/1‑71 (MQ=255)          gTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCg                                                           >  1:5527442/1‑71 (MQ=255)           tCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                                                   <  1:5225139/62‑1 (MQ=255)             ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCa                                                                 >  1:753132/1‑62 (MQ=255)             ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                                         >  1:2966344/1‑70 (MQ=255)             ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                                         >  1:3375138/1‑70 (MQ=255)             ctgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                                         >  1:1178530/1‑70 (MQ=255)             ctgctgcagTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGa                                                         >  1:562899/1‑70 (MQ=255)              tgctgctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCt                                                              <  1:1232043/64‑1 (MQ=255)                   ctgTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                                                  <  1:1119205/71‑1 (MQ=255)                      ttCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                                                <  1:1488244/70‑1 (MQ=255)                       tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg                                              >  1:2144533/1‑71 (MQ=255)                       tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg                                              >  1:5385737/1‑71 (MQ=255)                       tCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCgg                                              >  1:3291109/1‑71 (MQ=255)                        cTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAg                                                                >  1:4205704/1‑52 (MQ=255)                            aGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttttt                                                  <  1:5332446/62‑1 (MQ=255)                             gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                              >  1:3904211/1‑65 (MQ=9)                             gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                                        >  1:4713787/1‑71 (MQ=255)                             gTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTc                                        <  1:2381112/71‑1 (MQ=255)                              tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc                                       <  1:4341197/71‑1 (MQ=255)                              tCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTcc                                       <  1:1067665/71‑1 (MQ=255)                                aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                           <  1:862411/65‑1 (MQ=255)                                aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGtt                                           <  1:5478999/65‑1 (MQ=255)                                aGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt                                     >  1:4465790/1‑71 (MQ=255)                                 gCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGt                                                                   <  1:2426693/40‑1 (MQ=255)                                  cGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATcg                                   >  1:2599153/1‑71 (MQ=255)                                    cgTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                                <  1:5500462/55‑1 (MQ=12)                                      ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTc                                                 <  1:1855915/53‑1 (MQ=255)                                      ttGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTc                                                 <  1:1169838/53‑1 (MQ=255)                                        gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCa                                      >  1:3090896/1‑62 (MQ=255)                                        gAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCg                              <  1:1841906/70‑1 (MQ=255)                                          aaCGTACTGCGTAGCCAGATACAGGGATGGTCAGCTGCGGATATTTTTcc                                                <  1:4784597/50‑1 (MQ=255)                                           aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGagca                          <  1:2574704/71‑1 (MQ=255)                                           aCGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGag                            >  1:4723853/1‑69 (MQ=255)                                              tACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATAttt                                                    <  1:1550897/42‑1 (MQ=255)                                                cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                     >  1:2496566/3‑71 (MQ=12)                                                cggCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAt                      >  1:2145640/3‑70 (MQ=12)                                                  gCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTcc                                                <  1:4827450/42‑1 (MQ=255)                                                  gCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                   >  1:3894100/1‑71 (MQ=16)                                                   cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCAt                                     >  1:4987806/1‑52 (MQ=255)                                                   cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCAttt                    <  1:2534990/69‑1 (MQ=255)                                                   cGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCa                  >  1:686846/1‑71 (MQ=255)                                                      aGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCga               >  1:611247/1‑70 (MQ=12)                                                       gCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCgat              >  1:204410/1‑69 (MQ=12)                                                          agagACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtc            <  1:3476824/70‑1 (MQ=9)                                                          aGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtca           >  1:4707475/1‑71 (MQ=255)                                                           gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat          <  1:1890052/68‑1 (MQ=9)                                                           gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat          <  1:1208217/68‑1 (MQ=9)                                                           gagacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcat          <  1:3047823/68‑1 (MQ=9)                                                             tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc         <  1:4459557/70‑1 (MQ=255)                                                             tACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtcatc         <  1:3986411/70‑1 (MQ=255)                                                             gacacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATtcatta        >  1:2583400/2‑69 (MQ=9)                                                              acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTtc            >  1:2752349/1‑66 (MQ=255)                                                              acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc       >  1:125761/1‑71 (MQ=255)                                                              acacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCAc       >  1:378263/1‑71 (MQ=255)                                                               cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg      <  1:5515929/71‑1 (MQ=12)                                                               cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg      <  1:3994210/71‑1 (MQ=12)                                                               cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg      <  1:2610403/71‑1 (MQ=12)                                                               cacGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACg      <  1:2878476/71‑1 (MQ=12)                                                               cacGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACg      >  1:1900190/1‑71 (MQ=255)                                                                 cGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt    <  1:724340/71‑1 (MQ=255)                                                                  ggATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGtt    >  1:2256425/1‑70 (MQ=255)                                                                   gATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTaa  >  1:3215964/1‑71 (MQ=255)                                                                      |                                                                    AGTAATAACGTCCTGCTGCTGTTCTTTAGTCAGCGAGTTGAAACGTACTGCGTAGCCAGATACACGGATGGTCAGCTGCGGATATTTTTCCGGGTTTTCCATCGCGTCGAGCAGCATTTCACGGTTCATCACGTTAA  >  minE/607995‑608131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑