| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,259 | G→C | 9.4% | S665S (TCC→TCG) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,259 | 0 | G | C | 9.4% | 266.7 / 14.2 | 95 | S665S (TCC→TCG) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (46/40); new base C (7/2); total (53/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
ACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCT > minE/608190‑608329 | aCCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAgg < 1:2844479/71‑1 (MQ=255) agGTTGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGAtcc > 1:2780402/1‑68 (MQ=255) agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc < 1:5183340/71‑1 (MQ=255) ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc > 1:3288311/1‑70 (MQ=255) ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCa > 1:924811/1‑71 (MQ=255) ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCa > 1:4216897/1‑71 (MQ=255) gTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCAt > 1:266083/1‑71 (MQ=255) tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTa < 1:199760/71‑1 (MQ=255) ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAg > 1:1325124/1‑70 (MQ=255) ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAg > 1:2734750/1‑70 (MQ=255) ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAg > 1:1839091/1‑70 (MQ=255) tACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCg > 1:4288506/1‑71 (MQ=255) aCGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCg > 1:1604319/1‑70 (MQ=255) aCGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCg > 1:4504611/1‑70 (MQ=255) aCTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAg > 1:4495514/1‑70 (MQ=255) cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:3001695/71‑1 (MQ=255) cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:3577571/71‑1 (MQ=255) cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:601071/71‑1 (MQ=255) cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:4424500/71‑1 (MQ=255) cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:2603901/71‑1 (MQ=255) cgtcgtCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa < 1:747403/70‑1 (MQ=255) gtcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACg > 1:4515711/1‑71 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATc < 1:5622563/53‑1 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACgg > 1:1411211/1‑71 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACgg > 1:384793/1‑71 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTATAGCGTAAGCAAACgg < 1:1381964/71‑1 (MQ=255) gtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt > 1:2512603/1‑53 (MQ=255) gtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt > 1:2525290/1‑53 (MQ=255) gtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt > 1:4431174/1‑53 (MQ=255) gtcTTTACCCAGTGCGTT‑GGAACGATAGAGAAGGTGTAGGAGATACCATCtt > 1:1218642/1‑52 (MQ=255) tACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa > 1:2353965/1‑61 (MQ=255) tACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa > 1:2424067/1‑61 (MQ=255) tACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:350380/69‑1 (MQ=255) aCCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGt < 1:1894733/67‑1 (MQ=255) aCCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTa < 1:4161336/70‑1 (MQ=255) aCCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTa < 1:5430160/70‑1 (MQ=255) ccAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt < 1:4271542/44‑1 (MQ=255) ccAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGc < 1:4068465/62‑1 (MQ=255) aGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCttt > 1:3229642/1‑44 (MQ=255) aGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCttt > 1:1822003/1‑44 (MQ=255) aGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCttt > 1:2203233/1‑44 (MQ=255) gTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc < 1:291027/37‑1 (MQ=255) gTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc < 1:2119633/37‑1 (MQ=255) gTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCAt < 1:2279280/39‑1 (MQ=255) gTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg > 1:5103641/1‑71 (MQ=255) tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg < 1:666583/71‑1 (MQ=255) tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg > 1:3162435/1‑70 (MQ=255) tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg > 1:2595823/1‑70 (MQ=255) cGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg > 1:726501/1‑69 (MQ=255) cGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa < 1:184186/71‑1 (MQ=255) ttCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTa < 1:1140240/44‑1 (MQ=255) ggAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCa > 1:3755348/1‑70 (MQ=255) ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag < 1:1390292/71‑1 (MQ=255) ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag < 1:5653632/71‑1 (MQ=255) gAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCg < 1:2045071/38‑1 (MQ=21) aaCGATAGAGAATGTGTAGGAGATACCATCTTTAg < 1:1369106/35‑1 (MQ=255) aaCGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa > 1:4607131/1‑68 (MQ=255) aCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCa > 1:3865439/1‑49 (MQ=255) aCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAg > 1:256866/1‑50 (MQ=255) aCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCaat > 1:513774/1‑67 (MQ=255) aCGATTGAGAAGGTGTACGAGATCCCATATTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCaat > 1:4234740/1‑67 (MQ=255) cGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAg > 1:4943854/1‑49 (MQ=255) cGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAg > 1:4597779/1‑49 (MQ=255) cGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAg > 1:2378580/1‑49 (MQ=255) gATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa < 1:4060079/61‑1 (MQ=255) gATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa < 1:5255274/61‑1 (MQ=255) aTAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc < 1:1327759/71‑1 (MQ=255) ttgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc > 1:5678493/3‑70 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:2004201/38‑1 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc > 1:3348759/1‑70 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc < 1:4135293/70‑1 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc > 1:1762916/1‑70 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:4378990/1‑71 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:2969125/1‑71 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:5573347/1‑71 (MQ=255) gagaAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa > 1:2964523/1‑38 (MQ=255) gagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGc < 1:4579883/50‑1 (MQ=255) gaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg < 1:920718/71‑1 (MQ=255) aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc < 1:3033685/71‑1 (MQ=255) aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc < 1:3026919/71‑1 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:1318659/1‑48 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:1242477/1‑48 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:3013444/1‑48 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:3370390/1‑48 (MQ=255) gtgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc < 1:15115/62‑1 (MQ=255) gtgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCAcc > 1:397857/1‑71 (MQ=255) tgtAGTAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:4083921/39‑1 (MQ=255) tgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa < 1:2288181/54‑1 (MQ=255) tgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa < 1:5068583/54‑1 (MQ=255) gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt > 1:267150/1‑71 (MQ=255) tAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga > 1:324205/1‑54 (MQ=255) aGGAGATACCATCTTAAGCGAAAGCAAACGGCAGTTTAGc < 1:177471/40‑1 (MQ=38) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:5603934/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:3020729/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:1009061/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:1787219/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:3027479/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTa > 1:1595621/1‑37 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagaga > 1:5311233/1‑54 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCt > 1:415944/1‑71 (MQ=255) | ACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCT > minE/608190‑608329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |