| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,283 | A→G | 19.0% | F657F (TTT→TTC) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,283 | 0 | A | G | 19.0% | 209.3 / 39.3 | 100 | F657F (TTT→TTC) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base A (45/36); new base G (16/3); total (61/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGT > minE/608213‑608350 | cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:3577571/71‑1 (MQ=255)cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:2603901/71‑1 (MQ=255)cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:3001695/71‑1 (MQ=255)cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:601071/71‑1 (MQ=255)cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:3063704/71‑1 (MQ=255)cTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:4424500/71‑1 (MQ=255) cgtcgtCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa < 1:747403/70‑1 (MQ=255) gtcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACg > 1:4515711/1‑71 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACgg > 1:384793/1‑71 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACgg > 1:1411211/1‑71 (MQ=255) tcgtcTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTATAGCGTAAGCAAACgg < 1:1381964/71‑1 (MQ=255) tACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa > 1:2424067/1‑61 (MQ=255) tACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa > 1:2353965/1‑61 (MQ=255) aCCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGt < 1:1894733/67‑1 (MQ=255) aCCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTa < 1:5430160/70‑1 (MQ=255) ccAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGc < 1:4068465/62‑1 (MQ=255) gTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg > 1:5103641/1‑71 (MQ=255) tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg < 1:666583/71‑1 (MQ=255) tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg > 1:2595823/1‑70 (MQ=255) tGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACg > 1:3162435/1‑70 (MQ=255) cGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACgg > 1:726501/1‑69 (MQ=255) cGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa < 1:184186/71‑1 (MQ=255) ggAACGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCa > 1:3755348/1‑70 (MQ=255) ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag < 1:5653632/71‑1 (MQ=255) ggAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag < 1:1390292/71‑1 (MQ=255) aCGATTGAGAAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCa > 1:3865439/1‑49 (MQ=255) aCGATTGAGAAGGTGTACGAGATCCCATATTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCaat > 1:4234740/1‑67 (MQ=255) cGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAg > 1:4943854/1‑49 (MQ=255) cGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAg > 1:2378580/1‑49 (MQ=255) cGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAg > 1:4597779/1‑49 (MQ=255) gATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa < 1:4060079/61‑1 (MQ=255) gATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGaa < 1:5255274/61‑1 (MQ=255) aTAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc < 1:1327759/71‑1 (MQ=255) ttgagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGc > 1:5678493/3‑70 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCa < 1:2004201/38‑1 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc < 1:4135293/70‑1 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc > 1:1762916/1‑70 (MQ=255) tAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGc > 1:3348759/1‑70 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:5573347/1‑71 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:2969125/1‑71 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:4378990/1‑71 (MQ=255) gagaAGGTGTAGGAGATACCATCTTTAGCGTAAGCaaa > 1:2964523/1‑38 (MQ=255) gagaAGGTGTACGAGATCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGc < 1:4579883/50‑1 (MQ=255) gaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg < 1:920718/71‑1 (MQ=255) aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc < 1:3033685/71‑1 (MQ=255) aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc < 1:3026919/71‑1 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:3370390/1‑48 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:1242477/1‑48 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:1318659/1‑48 (MQ=255) ggTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAAc > 1:3013444/1‑48 (MQ=255) gtgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCAcc > 1:397857/1‑71 (MQ=255) tgtAGTAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:4083921/39‑1 (MQ=255) tgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa < 1:2288181/54‑1 (MQ=255) tgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCa < 1:5068583/54‑1 (MQ=255) gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt > 1:267150/1‑71 (MQ=255) tAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga > 1:324205/1‑54 (MQ=255) aGGAGATACCATCTTAAGCGAAAGCAAACGGCAGTTTAGc < 1:177471/40‑1 (MQ=38) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:1787219/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:3020729/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:1009061/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGtt < 1:5603934/35‑1 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTa > 1:1595621/1‑37 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCagaga > 1:5311233/1‑54 (MQ=255) ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCt > 1:415944/1‑71 (MQ=255) cgagaTCCCATCTTTGGCGTAGGTGAACGGCAGttt > 1:3768728/2‑36 (MQ=12) cgagaTCCCATCTTTGGCGTAGGTGAACGGCAGttt > 1:1605421/2‑36 (MQ=12) cgagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCa < 1:813430/49‑1 (MQ=255) gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg > 1:4114886/1‑68 (MQ=255) gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg > 1:1693457/1‑68 (MQ=255) gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg < 1:834402/71‑4 (MQ=255) gagaTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:4826911/1‑58 (MQ=255) gagaTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa > 1:3596066/1‑58 (MQ=255) aTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGt < 1:3959556/44‑1 (MQ=255) aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCaa > 1:498555/1‑35 (MQ=255) aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCaa > 1:2090346/1‑35 (MQ=255) aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCaa > 1:2515299/1‑35 (MQ=255) aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGa > 1:585966/1‑39 (MQ=255) ccATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc > 1:3762863/1‑70 (MQ=255) ccATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc > 1:3321072/1‑70 (MQ=255) ccATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTgg < 1:1633976/66‑1 (MQ=255) ccATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc > 1:526394/1‑70 (MQ=255) cATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa > 1:489970/1‑71 (MQ=255) aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc < 1:1258203/71‑1 (MQ=255) tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGATCACGAc > 1:432341/1‑70 (MQ=255) cTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAcca > 1:5093733/1‑70 (MQ=255) tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga < 1:1066885/40‑1 (MQ=255) tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCaga > 1:4230043/1‑40 (MQ=255) tttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCag > 1:3917764/1‑39 (MQ=255) ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt > 1:3836571/1‑70 (MQ=255) ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg > 1:1192396/1‑71 (MQ=255) ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCCTg > 1:4201025/1‑71 (MQ=255) tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc < 1:635675/67‑1 (MQ=255) tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt < 1:2513253/69‑1 (MQ=255) tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg > 1:3385579/1‑70 (MQ=255) cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc < 1:3595044/71‑1 (MQ=255) gTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg > 1:5471197/1‑71 (MQ=255) tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg < 1:2509113/70‑1 (MQ=255) aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc > 1:3140718/1‑59 (MQ=255) aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc > 1:4945308/1‑59 (MQ=255) aGCAAACGGCAGTTTAGAAACGGAAGTCAGAGAGGCTACTCCACCTTTCTGGTAACGACCGTGCATCGGGt > 1:4693906/1‑71 (MQ=255) | CTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGT > minE/608213‑608350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |