Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,319 T→G 30.0% A645A (GCA→GCC)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,3190TG30.0% 56.4 / 50.0 60A645A (GCA→GCC) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (23/19);  new base G (12/6);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACG  >  minE/608250‑608384
                                                                     |                                                                 
gaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg                                                                  <  1:920718/71‑1 (MQ=255)
 aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                                 <  1:3026919/71‑1 (MQ=255)
 aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                                 <  1:3033685/71‑1 (MQ=255)
    gtgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCAcc                                                              >  1:397857/1‑71 (MQ=255)
      gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt                                                            >  1:267150/1‑71 (MQ=255)
         ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCt                                                         >  1:415944/1‑71 (MQ=255)
          gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg                                                        >  1:1693457/1‑68 (MQ=255)
          gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg                                                        <  1:834402/71‑4 (MQ=255)
          gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg                                                        >  1:4114886/1‑68 (MQ=255)
               aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc                                                   >  1:5691076/1‑71 (MQ=255)
                ccATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc                                                   >  1:3321072/1‑70 (MQ=255)
                ccATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc                                                   >  1:3762863/1‑70 (MQ=255)
                ccATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTgg                                                       <  1:1633976/66‑1 (MQ=255)
                ccATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc                                                   >  1:526394/1‑70 (MQ=255)
                 cATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa                                                 >  1:196632/1‑71 (MQ=255)
                 cATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa                                                 >  1:489970/1‑71 (MQ=255)
                  aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc                                                <  1:1258203/71‑1 (MQ=255)
                   tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGATCACGAc                                                >  1:432341/1‑70 (MQ=255)
                    cTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAcca                                              >  1:5093733/1‑70 (MQ=255)
                      ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                             >  1:3836571/1‑70 (MQ=255)
                      ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg                                            >  1:1192396/1‑71 (MQ=255)
                      ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCCTg                                            >  1:4201025/1‑71 (MQ=255)
                       tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                               <  1:635675/67‑1 (MQ=255)
                       tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                             <  1:2513253/69‑1 (MQ=255)
                       tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg                                            >  1:3385579/1‑70 (MQ=255)
                          cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                        <  1:3595044/71‑1 (MQ=255)
                           gTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg                                       >  1:5471197/1‑71 (MQ=255)
                            tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg                                       <  1:2509113/70‑1 (MQ=255)
                              aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc                                                >  1:4945308/1‑59 (MQ=255)
                              aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc                                                >  1:3140718/1‑59 (MQ=255)
                              aGCAAACGGCAGTTTAGAAACGGAAGTCAGAGAGGCTACTCCACCTTTCTGGTAACGACCGTGCATCGGGt                                    >  1:4693906/1‑71 (MQ=255)
                                  aaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGc                                >  1:2058665/1‑71 (MQ=255)
                                   aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               >  1:321985/1‑70 (MQ=255)
                                   aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               <  1:945225/71‑2 (MQ=255)
                                   aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               >  1:2844125/1‑70 (MQ=255)
                                   aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               >  1:3138578/1‑70 (MQ=255)
                                   aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt                                                      >  1:2937546/1‑48 (MQ=255)
                                   aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                                >  1:1711072/1‑70 (MQ=255)
                                     ggCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGc                                           <  1:3407023/57‑1 (MQ=255)
                                     ggCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGc                                           <  1:2386775/57‑1 (MQ=255)
                                      gCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGccc                            <  1:1432672/71‑1 (MQ=255)
                                        aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                          <  1:3073683/71‑1 (MQ=255)
                                        aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                          <  1:1762056/71‑1 (MQ=255)
                                              gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                    <  1:2120626/71‑1 (MQ=255)
                                               cAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc                                                   >  1:1451238/1‑39 (MQ=255)
                                                  cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                <  1:5113031/71‑1 (MQ=255)
                                                   ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                            >  1:2368787/1‑58 (MQ=255)
                                                   ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                            >  1:1922208/1‑58 (MQ=255)
                                                    gAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgc              <  1:3688099/71‑1 (MQ=255)
                                                      aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc            >  1:980134/1‑71 (MQ=255)
                                                      aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc            >  1:3105067/1‑71 (MQ=255)
                                                        tCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                                    <  1:5463821/45‑1 (MQ=255)
                                                            tgagGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                                   <  1:900613/41‑1 (MQ=255)
                                                            agagGCTACTGCACCTTTCTGGTCACGACCGTGCAt                                         >  1:3128837/1‑36 (MQ=255)
                                                            agagGCTACTGCACCTTTCTGGTCACGACCGTGCAt                                         >  1:2995951/1‑36 (MQ=255)
                                                            agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac      <  1:2490337/71‑1 (MQ=255)
                                                            agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac      <  1:1399283/71‑1 (MQ=255)
                                                               ggCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga    >  1:2161674/1‑70 (MQ=255)
                                                                gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgacg  <  1:3342709/71‑1 (MQ=255)
                                                                 cTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAg                                 <  1:1676881/39‑1 (MQ=255)
                                                                     |                                                                 
GAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACG  >  minE/608250‑608384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑