Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,355	A→G	26.0%	G633G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,355	0	A	G	26.0%	71.0 / 28.5	50	G633G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (22/15);  new base G (4/9);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

GCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGT  >  minE/608288‑608425                                                                    |                                                                       gCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGccc                                                                     <  1:1432672/71‑1 (MQ=255)   aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                                                   <  1:3073683/71‑1 (MQ=255)   aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                                                                   <  1:1762056/71‑1 (MQ=255)         gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                                                             <  1:2120626/71‑1 (MQ=255)             cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                                                         <  1:5113031/71‑1 (MQ=255)              ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                                                                     >  1:2368787/1‑58 (MQ=255)              ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                                                                     >  1:1922208/1‑58 (MQ=255)               gAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgc                                                       <  1:3688099/71‑1 (MQ=255)                 aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                     >  1:3105067/1‑71 (MQ=255)                 aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc                                                     >  1:980134/1‑71 (MQ=255)                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                               <  1:1399283/71‑1 (MQ=255)                       agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac                                               <  1:2490337/71‑1 (MQ=255)                          ggCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                             >  1:2161674/1‑70 (MQ=255)                           gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgacg                                           <  1:3342709/71‑1 (MQ=255)                               cggCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCg                                       >  1:616796/3‑71 (MQ=255)                                ggCACCTTTGAGGTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCg                                       <  1:276826/69‑1 (MQ=255)                                 gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                             >  1:3639095/1‑63 (MQ=255)                                 gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                             >  1:3379603/1‑63 (MQ=255)                                 gCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                         >  1:933964/1‑67 (MQ=255)                                  cACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACgacgac                                         >  1:5403555/1‑66 (MQ=255)                                  cACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCt                                    >  1:5213759/1‑71 (MQ=255)                                  cACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCt                                    >  1:5464753/1‑71 (MQ=255)                                    ccTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCttg                                  <  1:2552236/71‑3 (MQ=255)                                       ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAg                                                  >  1:2966184/1‑52 (MQ=255)                                       ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                               >  1:4138635/1‑71 (MQ=255)                                       ttCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                               >  1:2646935/1‑71 (MQ=255)                                        tgcggTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgt                               <  1:1831786/67‑1 (MQ=255)                                        tCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgt                               <  1:2347125/70‑1 (MQ=255)                                         cTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga                                             >  1:2409864/1‑55 (MQ=255)                                            gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGgtgt                             <  1:502969/68‑1 (MQ=255)                                            gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                          >  1:3947601/1‑71 (MQ=255)                                            gTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTAc                          >  1:5106630/1‑71 (MQ=255)                                            gTCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAc                          <  1:1035538/71‑1 (MQ=255)                                             tCACGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCgta                              >  1:4677054/1‑65 (MQ=255)                                               aCGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTggg                                 >  1:928333/1‑61 (MQ=255)                                               aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccgg                       >  1:4037640/1‑69 (MQ=255)                                               aCGACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTAccg                        <  1:2079948/70‑2 (MQ=255)                                                 gACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGtt                     <  1:3841163/71‑1 (MQ=255)                                                 gACCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGtt                     <  1:1515024/71‑1 (MQ=255)                                                  aCCATGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGttt                    <  1:4754005/71‑1 (MQ=255)                                                     gTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCt                 <  1:2869956/71‑1 (MQ=255)                                                      tGCATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGtt                     >  1:5672208/1‑66 (MQ=255)                                                       gCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTa               >  1:4342200/1‑71 (MQ=255)                                                        cATCGGGTTAGCGCCCGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCtggc              <  1:1104124/71‑4 (MQ=255)                                                         aTCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTgg                                  >  1:2202266/1‑50 (MQ=255)                                                            gggTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATa          <  1:1332168/71‑1 (MQ=255)                                                               ttAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCg                                       <  1:1314312/39‑1 (MQ=255)                                                                 aGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACaa     >  1:4628740/1‑71 (MQ=255)                                                                   cACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACg   >  1:5220220/1‑71 (MQ=255)                                                                   cACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACg   <  1:3273572/71‑1 (MQ=255)                                                                    aCCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGt  <  1:1479695/71‑1 (MQ=255)                                                                    |                                                                       GCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGT  >  minE/608288‑608425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑