Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,445	A→C	14.8%	S603S (TCT→TCG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,445	0	A	C	14.8%	164.8 / 27.7	81	S603S (TCT→TCG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (36/33);  new base C (6/6);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGG  >  minE/608377‑608514                                                                     |                                                                      gCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa                                                                     >  1:1599268/1‑71 (MQ=255) gCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa                                                                     >  1:1605881/1‑71 (MQ=255) gCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa                                                                     >  1:2419006/1‑71 (MQ=255)  cACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGAc                                                                    <  1:5056468/71‑1 (MQ=255)    cgacgaCCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTg                                                                  <  1:1131484/71‑1 (MQ=255)    cgacgaCCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTg                                                                  <  1:985849/71‑1 (MQ=255)     gacgacCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACt                                                                   >  1:779657/1‑69 (MQ=255)       cgacCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGt                                                               >  1:1411661/1‑71 (MQ=255)        gacCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTc                                                              <  1:2463970/71‑1 (MQ=255)         acCGGCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCg                                                             <  1:998088/71‑1 (MQ=255)          ccGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCgg                                                            <  1:1327464/71‑1 (MQ=255)          ccGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg                                                            >  1:3422674/1‑71 (MQ=255)           cGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCggg                                                           <  1:3959433/71‑1 (MQ=255)           cGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCggg                                                           <  1:858281/71‑1 (MQ=255)           cGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCggg                                                           <  1:3416414/71‑1 (MQ=255)            gTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACa                                                                       >  1:1857874/1‑58 (MQ=255)            gTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACa                                                                       >  1:5010318/1‑58 (MQ=255)                      ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGta                                                              >  1:2900815/1‑56 (MQ=255)                      ttACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACg                                                >  1:4274323/1‑71 (MQ=255)                      ttACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGCAt                                                         <  1:4533783/62‑1 (MQ=255)                       tACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg                                                            <  1:4226166/58‑1 (MQ=255)                       tACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGt                                                    <  1:3955736/66‑1 (MQ=255)                        aCCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                               >  1:115970/1‑70 (MQ=255)                            gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                               >  1:3634530/1‑66 (MQ=255)                            gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                               >  1:1062633/1‑66 (MQ=255)                            gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                          >  1:4710746/1‑71 (MQ=255)                            gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                          >  1:2541054/1‑71 (MQ=255)                            gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                          >  1:2879597/1‑71 (MQ=255)                            gTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGgt                                          >  1:2102100/1‑71 (MQ=255)                             ttttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACa                                                                       <  1:2378405/41‑1 (MQ=255)                             ttttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACa                                                                       <  1:5667625/41‑1 (MQ=255)                             ttttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACa                                                                       <  1:38625/41‑1 (MQ=255)                             ttttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCAc                                                 <  1:4317281/63‑1 (MQ=255)                              tttCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACt                                                                   >  1:4245046/1‑44 (MQ=12)                               ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgtgt                                        <  1:4831545/70‑1 (MQ=255)                               ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGtgtg                                       >  1:1910693/1‑71 (MQ=255)                               ttCTTACCATACACAACGTTAGAAGAGATGGTCAGAACAGACTGAGTCGGGATAg                                                       >  1:3934166/1‑55 (MQ=255)                                tCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGa                                                                     <  1:2128709/40‑1 (MQ=255)                                    aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCa                                     <  1:3568046/68‑1 (MQ=255)                                    aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                   <  1:4449503/70‑1 (MQ=255)                                    aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                   >  1:1920407/1‑70 (MQ=255)                                     ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATATCGTCACGGTAGGTGTGCAGttt                                 <  1:82175/71‑1 (MQ=255)                                     ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                    <  1:5049511/68‑1 (MQ=255)                                       aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGt                                              <  1:3255532/56‑1 (MQ=255)                                       aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCt                               >  1:1650878/1‑71 (MQ=255)                                         acacAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGa                                                          >  1:5409775/1‑42 (MQ=255)                                         acacAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGa                                                          >  1:711807/1‑42 (MQ=255)                                         acacAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGa                                                          >  1:2274774/1‑42 (MQ=255)                                          caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg                                           >  1:471017/1‑56 (MQ=255)                                          cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCa                                                  >  1:5395537/1‑49 (MQ=255)                                          cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGaa                            >  1:2005261/1‑71 (MQ=255)                                             aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         >  1:932886/1‑71 (MQ=255)                                             aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         >  1:1961911/1‑71 (MQ=255)                                             aaCGTTAGAAGTGATGGTCAGAACAGACTCAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         <  1:1590691/71‑1 (MQ=255)                                              aCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         >  1:5092046/1‑70 (MQ=255)                                               cGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGGTTCTGAATTttc                       <  1:4513366/71‑1 (MQ=255)                                                 ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         <  1:478686/67‑1 (MQ=255)                                                 ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         <  1:1665726/67‑1 (MQ=255)                                                 ttAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttctt                     <  1:3191103/71‑1 (MQ=255)                                                   aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                   >  1:2664794/1‑71 (MQ=255)                                                   aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                   >  1:1923857/1‑71 (MQ=255)                                                   aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                   >  1:4144598/1‑71 (MQ=255)                                                   aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa                   >  1:3691980/1‑71 (MQ=255)                                                    gAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCAt                  <  1:3737269/71‑1 (MQ=255)                                                    gAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTGAATTTTCTTCAt                  <  1:903448/71‑1 (MQ=255)                                                        tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTttc                       <  1:1842762/62‑1 (MQ=255)                                                        tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                <  1:5430284/69‑1 (MQ=255)                                                        tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                <  1:2555051/69‑1 (MQ=255)                                                         gATGGTCAGAACAGACTGAGTCGGGATAGAGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc             >  1:1607965/1‑71 (MQ=255)                                                          aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAg                                            >  1:1619658/1‑39 (MQ=255)                                                          aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc             <  1:5169601/70‑1 (MQ=255)                                                          aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc             <  1:2557183/70‑1 (MQ=255)                                                          aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg            <  1:411506/71‑1 (MQ=255)                                                          aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg            >  1:4177682/1‑71 (MQ=255)                                                             gTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGGGTGCAGTTTCTGAATTTTCTTCATGAAACGtt          <  1:470384/70‑1 (MQ=255)                                                              tCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCt        <  1:836843/71‑1 (MQ=255)                                                               cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         >  1:3275993/1‑53 (MQ=255)                                                               cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                         >  1:512016/1‑53 (MQ=255)                                                                aGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTa       >  1:457559/1‑70 (MQ=255)                                                                 gAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTAcc     <  1:4320318/71‑1 (MQ=255)                                                                  aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc             <  1:727378/62‑1 (MQ=255)                                                                  aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc             <  1:3001829/62‑1 (MQ=255)                                                                  aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc             <  1:4152402/62‑1 (MQ=255)                                                                   aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                    >  1:5564359/1‑38 (MQ=255)                                                                   aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTg                              >  1:2980204/1‑44 (MQ=255)                                                                    cAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAgg  >  1:2523699/1‑71 (MQ=255)                                                                     |                                                                      GCACGACGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGG  >  minE/608377‑608514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑