Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,451	A→G	11.4%	T601T (ACT→ACC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,451	0	A	G	11.4%	200.4 / 14.6	79	T601T (ACT→ACC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base G (5/4);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCA  >  minE/608383‑608517                                                                     |                                                                   cgacCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGt                                                                  >  1:1411661/1‑71 (MQ=255)  gacCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTc                                                                 <  1:2463970/71‑1 (MQ=255)   acCGGCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCg                                                                <  1:998088/71‑1 (MQ=255)    ccGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCgg                                                               <  1:1327464/71‑1 (MQ=255)    ccGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg                                                               >  1:3422674/1‑71 (MQ=255)     cGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCggg                                                              <  1:858281/71‑1 (MQ=255)     cGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCggg                                                              <  1:3959433/71‑1 (MQ=255)     cGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCggg                                                              <  1:3416414/71‑1 (MQ=255)                ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGta                                                                 >  1:2900815/1‑56 (MQ=255)                ttACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACg                                                   >  1:4274323/1‑71 (MQ=255)                ttACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGCAt                                                            <  1:4533783/62‑1 (MQ=255)                 tACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAgg                                                               <  1:4226166/58‑1 (MQ=255)                 tACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGt                                                       <  1:3955736/66‑1 (MQ=255)                  aCCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                  >  1:115970/1‑70 (MQ=255)                      gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                  >  1:1062633/1‑66 (MQ=255)                      gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                  >  1:3634530/1‑66 (MQ=255)                      gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                             >  1:2879597/1‑71 (MQ=255)                      gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                             >  1:4710746/1‑71 (MQ=255)                      gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                             >  1:2541054/1‑71 (MQ=255)                      gTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGgt                                             >  1:2102100/1‑71 (MQ=255)                       ttttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCAc                                                    <  1:4317281/63‑1 (MQ=255)                         ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgtgt                                           <  1:4831545/70‑1 (MQ=255)                         ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGtgtg                                          >  1:1910693/1‑71 (MQ=255)                         ttCTTACCATACACAACGTTAGAAGAGATGGTCAGAACAGACTGAGTCGGGATAg                                                          >  1:3934166/1‑55 (MQ=255)                              aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCa                                        <  1:3568046/68‑1 (MQ=255)                              aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                      <  1:4449503/70‑1 (MQ=255)                              aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                      >  1:1920407/1‑70 (MQ=255)                               ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATATCGTCACGGTAGGTGTGCAGttt                                    <  1:82175/71‑1 (MQ=255)                               ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                       <  1:5049511/68‑1 (MQ=255)                                 aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGt                                                 <  1:3255532/56‑1 (MQ=255)                                 aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCt                                  >  1:1650878/1‑71 (MQ=255)                                   acacAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGa                                                             >  1:5409775/1‑42 (MQ=255)                                   acacAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGa                                                             >  1:711807/1‑42 (MQ=255)                                   acacAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGa                                                             >  1:2274774/1‑42 (MQ=255)                                    caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg                                              >  1:471017/1‑56 (MQ=255)                                    cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCa                                                     >  1:5395537/1‑49 (MQ=255)                                    cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGaa                               >  1:2005261/1‑71 (MQ=255)                                       aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            >  1:1961911/1‑71 (MQ=255)                                       aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            >  1:932886/1‑71 (MQ=255)                                       aaCGTTAGAAGTGATGGTCAGAACAGACTCAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            <  1:1590691/71‑1 (MQ=255)                                        aCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            >  1:5092046/1‑70 (MQ=255)                                         cGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGGTTCTGAATTttc                          <  1:4513366/71‑1 (MQ=255)                                           ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            <  1:478686/67‑1 (MQ=255)                                           ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            <  1:1665726/67‑1 (MQ=255)                                           ttAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttctt                        <  1:3191103/71‑1 (MQ=255)                                             aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                      >  1:1923857/1‑71 (MQ=255)                                             aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                      >  1:2664794/1‑71 (MQ=255)                                             aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                      >  1:4144598/1‑71 (MQ=255)                                             aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa                      >  1:3691980/1‑71 (MQ=255)                                              gAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCAt                     <  1:3737269/71‑1 (MQ=255)                                              gAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTGAATTTTCTTCAt                     <  1:903448/71‑1 (MQ=255)                                                  tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTttc                          <  1:1842762/62‑1 (MQ=255)                                                  tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                   <  1:2555051/69‑1 (MQ=255)                                                  tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                   <  1:5430284/69‑1 (MQ=255)                                                   gATGGTCAGAACAGACTGAGTCGGGATAGAGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                >  1:1607965/1‑71 (MQ=255)                                                    aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAg                                               >  1:1619658/1‑39 (MQ=255)                                                    aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                <  1:2557183/70‑1 (MQ=255)                                                    aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                <  1:5169601/70‑1 (MQ=255)                                                    aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg               >  1:4177682/1‑71 (MQ=255)                                                    aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg               <  1:411506/71‑1 (MQ=255)                                                       gTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGGGTGCAGTTTCTGAATTTTCTTCATGAAACGtt             <  1:470384/70‑1 (MQ=255)                                                        tCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCt           <  1:836843/71‑1 (MQ=255)                                                         cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            >  1:512016/1‑53 (MQ=255)                                                         cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            >  1:3275993/1‑53 (MQ=255)                                                          aGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTa          >  1:457559/1‑70 (MQ=255)                                                           gAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTAcc        <  1:4320318/71‑1 (MQ=255)                                                            aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                <  1:3001829/62‑1 (MQ=255)                                                            aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                <  1:727378/62‑1 (MQ=255)                                                            aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                <  1:4152402/62‑1 (MQ=255)                                                             aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                       >  1:5564359/1‑38 (MQ=255)                                                             aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTg                                 >  1:2980204/1‑44 (MQ=255)                                                              cAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAgg     >  1:2523699/1‑71 (MQ=255)                                                                 aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                <  1:1551903/57‑1 (MQ=255)                                                                 aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCa  <  1:5237242/71‑1 (MQ=255)                                                                 aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCa  <  1:712001/71‑1 (MQ=255)                                                                 aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGATCTACCAGGTc   >  1:2196096/1‑70 (MQ=255)                                                                  cTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            <  1:4296603/44‑1 (MQ=255)                                                                  cTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            <  1:4267765/44‑1 (MQ=255)                                                                   tGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                            <  1:1389460/43‑1 (MQ=255)                                                                     |                                                                   CGACCGTCTGGGGTGTTACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCA  >  minE/608383‑608517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑