Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,652	A→C	7.1%	A534A (GCT→GCG) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,652	0	A	C	7.1%	218.4 / 9.6	85	A534A (GCT→GCG) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (41/38);  new base C (2/4);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCA  >  minE/608583‑608722                                                                      |                                                                       gTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcag                                                                       >  1:3606078/1‑71 (MQ=255) gTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcag                                                                       >  1:719439/1‑71 (MQ=255)    gATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcagcag                                                                    >  1:2594567/1‑71 (MQ=255)    gATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcagca                                                                     <  1:1947354/70‑1 (MQ=255)     aTAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCagcagc                                                                   <  1:4490458/71‑1 (MQ=255)      tAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcagcag                                                                    >  1:2958687/1‑69 (MQ=255)      tAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCagcagc                                                                   >  1:3295102/1‑70 (MQ=255)      tAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCagcagc                                                                   >  1:4181604/1‑70 (MQ=255)         ccAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACg                                                               <  1:827431/71‑1 (MQ=255)          cAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCaa                                                                 >  1:4241703/1‑68 (MQ=255)           aGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGa                                                             >  1:4964313/1‑71 (MQ=255)            gACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGa                                                             >  1:2606141/1‑70 (MQ=255)              ccGTTTTCGTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAg                                                          <  1:3184799/71‑1 (MQ=255)               cGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcagca                                                                     >  1:798317/1‑59 (MQ=255)               cGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACa                                                           >  1:5132053/1‑69 (MQ=255)               cGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGa                                                         <  1:4881319/71‑1 (MQ=255)                 tCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCagcagc                                                                   <  1:2299178/59‑1 (MQ=255)                   ttCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAg                                                     >  1:5559560/1‑71 (MQ=255)                   ttCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAg                                                     >  1:2221473/1‑71 (MQ=255)                     cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                    <  1:5559538/70‑1 (MQ=255)                     cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                    <  1:525254/70‑1 (MQ=255)                     cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                    <  1:829012/70‑1 (MQ=255)                      gTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGc                                                    <  1:5654942/69‑1 (MQ=255)                       tCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGAt                                                 <  1:4089282/71‑1 (MQ=255)                         aCGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTcagc                                                                      >  1:1254233/1‑48 (MQ=255)                         aCGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATaa                                               >  1:4233397/1‑70 (MQ=255)                         aCGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAc                                               >  1:1333560/1‑71 (MQ=255)                           gAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACg                                                               <  1:914416/53‑1 (MQ=255)                           gAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACca                                             <  1:463864/71‑1 (MQ=255)                           gAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACca                                             <  1:2480866/71‑1 (MQ=255)                           gAATCGATTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGAc                                                            <  1:4183711/56‑1 (MQ=255)                             aTCGGTTTAACTTTCGCATATTTTATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACcaca                                           <  1:3384186/71‑1 (MQ=255)                             aTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACcaca                                           <  1:2769197/71‑1 (MQ=255)                              tCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                          >  1:5277265/1‑71 (MQ=255)                                 gTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGcc                                       <  1:1521978/71‑1 (MQ=255)                                 gTTAAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGc                                        <  1:3387994/70‑1 (MQ=255)                                   ttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCAt                                     >  1:5109093/1‑71 (MQ=255)                                    tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                    >  1:5075723/1‑71 (MQ=255)                                    tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                    >  1:3085328/1‑71 (MQ=255)                                    tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                    >  1:115620/1‑71 (MQ=255)                                     aaCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                    >  1:2842431/1‑70 (MQ=255)                                       cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                      <  1:38620/50‑1 (MQ=255)                                       cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                      <  1:4223321/50‑1 (MQ=255)                                       cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                      <  1:4423978/50‑1 (MQ=255)                                       cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                      <  1:758341/50‑1 (MQ=255)                                       cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                 <  1:117589/71‑1 (MQ=255)                                        tttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                >  1:2713393/1‑71 (MQ=255)                                         ttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                 >  1:5359604/1‑69 (MQ=255)                                         ttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                               >  1:4107728/1‑71 (MQ=255)                                          tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                <  1:2585078/69‑1 (MQ=255)                                          tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                <  1:2390300/69‑1 (MQ=255)                                          gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                              <  1:373464/69‑2 (MQ=255)                                           cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATgg                                   >  1:1881742/1‑65 (MQ=255)                                           cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATgg                                   >  1:1824049/1‑65 (MQ=255)                                            gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGAt                            <  1:937516/71‑1 (MQ=255)                                             cttatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                           <  1:2431491/69‑1 (MQ=255)                                             cATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                           <  1:920176/71‑1 (MQ=255)                                             cATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGa                                                  >  1:1089330/1‑48 (MQ=255)                                               tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACa                                                           <  1:3466546/37‑1 (MQ=255)                                               tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACa                                                           <  1:4210037/37‑1 (MQ=255)                                               tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGAt                                                 <  1:1127564/47‑1 (MQ=255)                                               tatTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGcccg                                                     <  1:1613128/43‑3 (MQ=12)                                                atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                    <  1:574690/43‑1 (MQ=255)                                                atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACg                        >  1:1149481/1‑71 (MQ=255)                                                 tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAg                                                          >  1:3417255/1‑36 (MQ=255)                                                 tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAg                                                          >  1:1767876/1‑36 (MQ=255)                                                 tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                  <  1:218253/44‑1 (MQ=255)                                                 tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGt                       >  1:3464094/1‑71 (MQ=255)                                                 tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGt                       >  1:4624953/1‑71 (MQ=255)                                                 tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGt                       >  1:4120457/1‑71 (MQ=255)                                                 tttGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGc                                                    >  1:1975401/1‑42 (MQ=255)                                                  ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGt                       >  1:4482926/1‑70 (MQ=255)                                                  ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGt                       >  1:3549713/1‑70 (MQ=255)                                                   tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTc                      <  1:4495706/70‑1 (MQ=255)                                                   tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACACCATGGTGCGGATAACGTCa                     >  1:1826472/1‑71 (MQ=255)                                                      ttGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACgg                  <  1:709857/71‑1 (MQ=255)                                                       tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGt                 >  1:3881570/1‑71 (MQ=255)                                                        gCAGACAGGGAGTCAGCAGCGACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTc                >  1:1409918/1‑71 (MQ=255)                                                        gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATaa                          >  1:2960014/1‑61 (MQ=255)                                                        gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATaa                          >  1:5666001/1‑61 (MQ=255)                                                        gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTc                >  1:5365467/1‑71 (MQ=255)                                                        gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTc                >  1:4431275/1‑71 (MQ=255)                                                        gCAGACAGGGAGTCAGCAGCAACGGACAGAACAGCGATACCACACGCCATGGTGCGGATaa                          >  1:898897/1‑61 (MQ=255)                                                         cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTc                <  1:70206/70‑1 (MQ=255)                                                         cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCg               >  1:3893114/1‑71 (MQ=255)                                                           gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCgcg             <  1:994016/71‑3 (MQ=255)                                                           gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTg             <  1:2664318/71‑1 (MQ=255)                                                            aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTg             >  1:4380746/1‑70 (MQ=255)                                                            aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGc            <  1:259841/71‑1 (MQ=255)                                                             cAGGGAGTCAGCAGCACCGGACAGACCAGCGATACcaca                                           >  1:4979632/1‑39 (MQ=255)                                                             cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGc            <  1:5063168/70‑1 (MQ=255)                                                             cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCa           <  1:2870297/71‑1 (MQ=255)                                                              aGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAg          >  1:4929491/1‑71 (MQ=255)                                                                ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCa           >  1:767944/1‑68 (MQ=255)                                                                ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCa           >  1:4325930/1‑68 (MQ=255)                                                                    tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCAt    <  1:5488704/71‑1 (MQ=255)                                                                      agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAg          >  1:4966893/1‑63 (MQ=255)                                                                      agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCAt    >  1:2339797/1‑69 (MQ=255)                                                                      agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCAt    >  1:3859894/1‑69 (MQ=255)                                                                      agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCa  <  1:5218487/71‑1 (MQ=255)                                                                      |                                                                       GTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCA  >  minE/608583‑608722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑