Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,700	G→A	10.5%	D518D (GAC→GAT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,700	0	G	A	10.5%	262.4 / 15.3	95	D518D (GAC→GAT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (52/33);  new base A (4/6);  total (56/39)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

ATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTT  >  minE/608630‑608768                                                                        |                                                                     atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                                      >  1:1149481/1‑71 (MQ=255)  tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                     >  1:4624953/1‑71 (MQ=255)  tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                     >  1:3464094/1‑71 (MQ=255)  tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                     >  1:4120457/1‑71 (MQ=255)   ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                     >  1:3549713/1‑70 (MQ=255)   ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                     >  1:4482926/1‑70 (MQ=255)    tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTc                                                                    <  1:4495706/70‑1 (MQ=255)    tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACACCATGGTGCGGATA‑ACGTCa                                                                   >  1:1826472/1‑71 (MQ=255)       ttGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                                <  1:709857/71‑1 (MQ=255)        tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                               >  1:3881570/1‑71 (MQ=255)         gCAGACAGGGAGTCAGCAGCGACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                              >  1:1409918/1‑71 (MQ=255)         gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                              >  1:5365467/1‑71 (MQ=255)         gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                              >  1:4431275/1‑71 (MQ=255)          cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                              <  1:70206/70‑1 (MQ=255)          cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCg                                                             >  1:3893114/1‑71 (MQ=255)            gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCgcg                                                           <  1:994016/71‑3 (MQ=255)            gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                           <  1:2664318/71‑1 (MQ=255)             aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                           >  1:4380746/1‑70 (MQ=255)             aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                                                          <  1:259841/71‑1 (MQ=255)              cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                                                          <  1:5063168/70‑1 (MQ=255)              cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                         <  1:2870297/71‑1 (MQ=255)               aGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                                                        >  1:4929491/1‑71 (MQ=255)                 ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                         >  1:767944/1‑68 (MQ=255)                 ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                         >  1:4325930/1‑68 (MQ=255)                     tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  <  1:5488704/71‑1 (MQ=255)                       agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                                                        >  1:4966893/1‑63 (MQ=255)                       agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  >  1:3859894/1‑69 (MQ=255)                       agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  >  1:2339797/1‑69 (MQ=255)                       agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                                <  1:5218487/71‑1 (MQ=255)                              aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                            >  1:606469/1‑68 (MQ=255)                              aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                            >  1:5597367/1‑68 (MQ=255)                              aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGc                                         >  1:225641/1‑71 (MQ=255)                               ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                                        >  1:2536424/3‑71 (MQ=255)                                ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                       <  1:203083/71‑1 (MQ=255)                                  aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                     >  1:190803/1‑71 (MQ=255)                                        ccgCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTg                               >  1:2590246/3‑71 (MQ=255)                                        cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                       >  1:449662/1‑63 (MQ=255)                                        cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                       >  1:4797998/1‑63 (MQ=255)                                        cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                       >  1:4804471/1‑63 (MQ=255)                                        cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                       >  1:789345/1‑63 (MQ=255)                                        cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                               <  1:4899071/71‑1 (MQ=255)                                        cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGGCGTGCAGCGCCAt                                                  <  1:4551539/52‑1 (MQ=255)                                          gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  <  1:5505039/50‑1 (MQ=255)                                          gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  <  1:4739138/50‑1 (MQ=255)                                          gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  <  1:774347/50‑1 (MQ=255)                                          gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  <  1:1076884/50‑1 (MQ=255)                                          gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                             >  1:1757715/1‑71 (MQ=255)                                           cGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                  <  1:3946373/49‑1 (MQ=255)                                            gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                           >  1:5614179/1‑39 (MQ=255)                                            gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                              >  1:2844041/1‑52 (MQ=255)                                            gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                                >  1:761863/1‑50 (MQ=255)                                            gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTAc                            >  1:4218048/1‑70 (MQ=255)                                            gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACt                           >  1:4981778/1‑71 (MQ=255)                                             aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                      >  1:2933757/1‑59 (MQ=255)                                              tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgc                                                       >  1:350219/1‑41 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                             >  1:2049538/1‑49 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                             >  1:1411553/1‑49 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                               <  1:1775790/63‑1 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                               <  1:4109552/63‑1 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                        <  1:1880320/70‑1 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                        <  1:4063157/70‑1 (MQ=255)                                                ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc                       <  1:4524482/71‑1 (MQ=255)                                                 cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                        <  1:3882643/69‑1 (MQ=255)                                                 cacaCGCCATCGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                              >  1:4626461/1‑47 (MQ=255)                                                  acacGCCATGGTGCTGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                     <  1:5588762/71‑1 (MQ=255)                                                  acacGCCATGGTGCGGATG‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                     <  1:5425087/71‑1 (MQ=255)                                                  acacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                             >  1:2945116/1‑47 (MQ=255)                                                  acacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                             >  1:5594075/1‑47 (MQ=255)                                                   catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg                    <  1:4083297/68‑1 (MQ=255)                                                   catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg                    <  1:3102444/68‑1 (MQ=255)                                                   catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg                    <  1:3557553/68‑1 (MQ=255)                                                   cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg                    >  1:3314966/1‑71 (MQ=255)                                                    acGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGc                   <  1:3358513/71‑1 (MQ=255)                                                     tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                                 <  1:1138975/39‑1 (MQ=9)                                                     tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                                 <  1:965006/39‑1 (MQ=9)                                                     tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                                 <  1:126045/39‑1 (MQ=9)                                                     tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                  >  1:3838314/2‑71 (MQ=255)                                                     tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                  >  1:568748/2‑71 (MQ=255)                                                       ccATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg                <  1:4580196/71‑1 (MQ=255)                                                        cATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGt               <  1:4603465/71‑1 (MQ=255)                                                         aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg                    <  1:3682641/65‑1 (MQ=255)                                                         aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg                    <  1:5161873/65‑1 (MQ=255)                                                            gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                                        >  1:4085135/1‑42 (MQ=255)                                                            gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                      >  1:4631848/1‑44 (MQ=255)                                                            gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTg           <  1:1283842/71‑1 (MQ=255)                                                             tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                            >  1:3987840/1‑37 (MQ=255)                                                                ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAg             >  1:697820/1‑65 (MQ=255)                                                                  aTA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatga      >  1:1649975/1‑70 (MQ=255)                                                                   tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                     >  1:3158117/1‑38 (MQ=255)                                                                   tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                     >  1:1718547/1‑38 (MQ=255)                                                                   tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGatgatg    >  1:2485028/1‑71 (MQ=255)                                                                    a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGTCTTc                                      >  1:1685677/1‑36 (MQ=255)                                                                    a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt   >  1:3993825/1‑71 (MQ=255)                                                                    a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt   >  1:5158606/1‑71 (MQ=255)                                                                      aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt  <  1:392572/71‑1 (MQ=255)                                                                       cGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt  >  1:3245952/1‑70 (MQ=255)                                                                        |                                                                     ATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTT  >  minE/608630‑608768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑