| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,766 | G→A | 31.4% | N496N (AAC→AAT) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,766 | 0 | G | A | 31.4% | 300.7 / 175.4 | 223 | N496N (AAC→AAT) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (77/76); new base A (28/42); total (105/118) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01 | |||||||||||
Only 100 of 236 total aligned reads displayed.
TAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCA > minE/608696‑608835 | acatCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATAtt < 1:4782496/68‑1 (MQ=255) aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt < 1:392572/71‑1 (MQ=255) catCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATAtt > 1:3770710/3‑70 (MQ=255) atCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTca < 1:2054779/70‑1 (MQ=255) atCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTca < 1:5568922/70‑1 (MQ=255) aCGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg < 1:2905936/71‑1 (MQ=255) aCGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcagcg > 1:323897/1‑69 (MQ=255) aCGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcagcg > 1:2214593/1‑69 (MQ=255) cgatCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcag > 1:1028952/4‑68 (MQ=255) cgatCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcag > 1:3621482/4‑68 (MQ=255) gTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag > 1:3504231/1‑71 (MQ=255) gTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag > 1:3317045/1‑71 (MQ=255) gCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg > 1:172179/1‑71 (MQ=255) gCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTca < 1:4621510/60‑1 (MQ=255) gCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg > 1:5244114/1‑71 (MQ=255) gCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg > 1:2277605/1‑71 (MQ=255) cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg < 1:1965890/70‑1 (MQ=255) cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGt < 1:4858122/71‑1 (MQ=255) cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGt < 1:470009/71‑1 (MQ=255) cAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg > 1:1228218/1‑70 (MQ=255) cAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg < 1:2351756/70‑1 (MQ=255) gcgcCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATAtt < 1:1676327/55‑1 (MQ=255) cgcCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACt < 1:1537417/71‑1 (MQ=255) cgcCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACt < 1:2579433/71‑1 (MQ=255) gcCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg < 1:3739247/71‑1 (MQ=255) cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg > 1:1419436/1‑69 (MQ=255) cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg > 1:2101143/1‑69 (MQ=255) cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt < 1:609636/71‑1 (MQ=255) tCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTcag > 1:3070682/1‑52 (MQ=255) tCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgca < 1:1877917/56‑1 (MQ=255) tCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgca < 1:217065/56‑1 (MQ=255) agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGttt > 1:2964557/1‑68 (MQ=255) agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc > 1:4505278/1‑71 (MQ=255) agagagGCTTCGTAGCTGTACTTGTCGAGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc > 1:3157256/1‑71 (MQ=255) gagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag > 1:974728/1‑71 (MQ=255) agGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg < 1:2750183/48‑1 (MQ=255) agGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg < 1:928533/48‑1 (MQ=255) tCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTc > 1:5482621/1‑39 (MQ=38) tCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTc > 1:536302/1‑39 (MQ=38) tCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTc > 1:4167962/1‑71 (MQ=255) cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcag > 1:2385686/1‑40 (MQ=255) aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgctgt < 1:4910145/43‑1 (MQ=255) aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCAt > 1:2913572/1‑70 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa > 1:3949930/1‑44 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa > 1:1453534/1‑44 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc < 1:824590/61‑1 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg > 1:4338760/1‑70 (MQ=255) cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg > 1:3284711/1‑69 (MQ=255) tGTACTTGTCGTGCATGTAGTGAATGATATTcagcgc > 1:4978199/1‑37 (MQ=25) gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt < 1:988713/49‑1 (MQ=255) gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt > 1:4273800/1‑71 (MQ=255) gTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt > 1:1201001/1‑71 (MQ=255) tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:565187/53‑1 (MQ=255) tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:2415906/53‑1 (MQ=255) tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:1791742/53‑1 (MQ=255) tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAg < 1:648144/69‑1 (MQ=255) tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg < 1:808091/71‑1 (MQ=255) tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg < 1:5146292/71‑1 (MQ=255) tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg < 1:4662897/71‑1 (MQ=255) aCTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa > 1:538167/1‑71 (MQ=255) aCTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgca < 1:912092/48‑3 (MQ=255) cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:4455116/51‑1 (MQ=255) ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca < 1:284552/52‑1 (MQ=255) ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca < 1:531762/52‑1 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca > 1:317845/1‑51 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca > 1:4194749/1‑51 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:3390186/1‑69 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:3428816/1‑69 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCACTGCAGTGATGTACTGTTTagcca > 1:395611/1‑51 (MQ=255) tGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACAGCCAGCCAGTCCATGAAGTGAt > 1:1192260/1‑69 (MQ=255) gTCTTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:1123840/69‑1 (MQ=255) gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTc > 1:5177967/1‑57 (MQ=255) gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc < 1:5453829/69‑1 (MQ=255) gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc < 1:2985682/69‑1 (MQ=255) gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc < 1:4102748/69‑1 (MQ=255) gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:2454362/69‑1 (MQ=255) tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga < 1:2636195/70‑3 (MQ=255) cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg > 1:5019723/1‑71 (MQ=255) gTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt > 1:3791247/1‑41 (MQ=255) gTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt > 1:2618236/1‑40 (MQ=255) cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATgcgc < 1:35490/70‑1 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcaagc < 1:558436/44‑1 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg < 1:3589835/53‑1 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc > 1:4003165/1‑70 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc > 1:3015956/1‑70 (MQ=255) tAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCa < 1:1974467/49‑1 (MQ=255) tAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa < 1:4580983/70‑1 (MQ=255) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa > 1:770928/1‑53 (MQ=12) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa > 1:1852218/1‑53 (MQ=12) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:1008395/59‑1 (MQ=255) gTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCa < 1:2696829/71‑1 (MQ=255) gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa > 1:2323210/1‑71 (MQ=255) tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc < 1:2240940/71‑1 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTagccag < 1:921637/36‑1 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa > 1:5534629/1‑47 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAg > 1:3481864/1‑49 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:1952591/1‑53 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt < 1:2768294/70‑1 (MQ=255) atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACttt > 1:4764583/1‑70 (MQ=255) tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg < 1:5018171/41‑1 (MQ=255) | TAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCA > minE/608696‑608835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |