BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	608,775	A→G	27.7%	T493T (ACT→ACC) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	608,775	0	A	G	27.7%	263.4 / 120.5	173	T493T (ACT→ACC) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (66/59); new base G (16/32); total (82/91)
Fisher's exact test for biased strand distribution p-value = 2.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 201 total aligned reads displayed.

GTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGG  >  minE/608706‑608844

gttgTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                                                      <  1:4200103/68‑1 (MQ=255)

 tCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagt                                                                     <  1:3953239/71‑1 (MQ=255)

 tCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagt                                                                     <  1:3191561/71‑1 (MQ=255)

 tCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagt                                                                     >  1:1684569/1‑71 (MQ=255)

   gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcagtg                                                                    <  1:1358362/70‑1 (MQ=255)

   gTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa                                                                   >  1:1885736/1‑71 (MQ=255)

     gCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg                                                                 >  1:2277605/1‑71 (MQ=255)

      cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg                                                                 <  1:4888514/70‑1 (MQ=255)

      cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg                                                                 <  1:1965890/70‑1 (MQ=255)

      cAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGt                                                                <  1:470009/71‑1 (MQ=255)

      cAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg                                                                 <  1:2351756/70‑1 (MQ=255)

         cgcCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACt                                                             <  1:2579433/71‑1 (MQ=255)

          gcCATCAGAGAGTCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                            <  1:3744199/71‑1 (MQ=255)

          gcCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                            <  1:3488505/71‑1 (MQ=255)

          gcCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                            <  1:2095007/71‑1 (MQ=255)

            cATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgca                                                          <  1:1111821/71‑3 (MQ=255)

            cATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgca                                                          <  1:2729436/71‑3 (MQ=255)

            cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTg                                                            >  1:2107867/1‑69 (MQ=255)

            cATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                                                          <  1:609636/71‑1 (MQ=255)

             aTCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgcac                                                         <  1:4290162/71‑4 (MQ=255)

              tCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgca                                                                       <  1:217065/56‑1 (MQ=255)

               cAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                                                       >  1:1534630/1‑71 (MQ=255)

                agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGttt                                                         >  1:2964557/1‑68 (MQ=255)

                agagagGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                      >  1:4505278/1‑71 (MQ=255)

                agagagGCTTCGTAGCTGTACTTGTCGAGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                      >  1:3157256/1‑71 (MQ=255)

                          cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTc                                             <  1:5058023/70‑1 (MQ=255)

                            tAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTa                                                        <  1:5263774/57‑1 (MQ=255)

                             aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                                                   >  1:4998409/1‑61 (MQ=255)

                             aGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCAt                                          >  1:2913572/1‑70 (MQ=255)

                              gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGa                                                                   >  1:4110529/1‑44 (MQ=255)

                              gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                  <  1:824590/61‑1 (MQ=255)

                              gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc                                                  <  1:5332163/61‑1 (MQ=255)

                              gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                         <  1:1396075/70‑1 (MQ=255)

                              gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                         >  1:4530813/1‑70 (MQ=255)

                              gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                         >  1:4338760/1‑70 (MQ=255)

                                 gTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt                                     >  1:1201001/1‑71 (MQ=255)

                                  tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                      <  1:1791742/53‑1 (MQ=255)

                                  tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAg                                      <  1:648144/69‑1 (MQ=255)

                                  tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg                                    <  1:808091/71‑1 (MQ=255)

                                  tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg                                    <  1:5146292/71‑1 (MQ=255)

                                   aCTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa                                   >  1:538167/1‑71 (MQ=255)

                                   aCTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa                                   >  1:5543885/1‑71 (MQ=255)

                                   aCTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTgca                                                          <  1:912092/48‑3 (MQ=255)

                                    cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc                                                      <  1:4455116/51‑1 (MQ=255)

                                    cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                  <  1:4667816/71‑1 (MQ=255)

                                    cTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg                                      >  1:5651246/1‑67 (MQ=255)

                                     ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca                                                    <  1:3823966/52‑1 (MQ=255)

                                     ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca                                                    <  1:284552/52‑1 (MQ=255)

                                     ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                                 >  1:5420974/1‑71 (MQ=255)

                                     ttGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg                                                                 <  1:986249/39‑1 (MQ=16)

                                     ttGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATg                                                                 <  1:2321909/39‑1 (MQ=16)

                                      tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTTATGTACTGTTTagcca                                                    >  1:4660148/1‑51 (MQ=255)

                                      tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                  >  1:3394917/1‑69 (MQ=255)

                                      tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                  >  1:417894/1‑69 (MQ=255)

                                      tGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                 >  1:1219189/1‑70 (MQ=255)

                                       gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc                                <  1:2236535/70‑1 (MQ=255)

                                       gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                                 <  1:5453829/69‑1 (MQ=255)

                                       gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                                 <  1:2985682/69‑1 (MQ=255)

                                       gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                               >  1:3310956/1‑71 (MQ=255)

                                       gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                 <  1:2454362/69‑1 (MQ=255)

                                        tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc                                 <  1:3352880/68‑1 (MQ=255)

                                        tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCAt                              >  1:4930906/1‑71 (MQ=255)

                                        tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga                               <  1:2636195/70‑3 (MQ=255)

                                         cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg                             >  1:5019602/1‑71 (MQ=255)

                                          gTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGt                                                           >  1:2618236/1‑40 (MQ=255)

                                           tGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGa                                        <  1:3970828/58‑1 (MQ=255)

                                             cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATgcgc                          <  1:35490/70‑1 (MQ=255)

                                             cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                         <  1:3455623/71‑1 (MQ=255)

                                             cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                         >  1:5178894/1‑71 (MQ=255)

                                               tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg                                         <  1:3589835/53‑1 (MQ=255)

                                               tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                        >  1:4003165/1‑70 (MQ=255)

                                               tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                        >  1:738708/1‑70 (MQ=255)

                                                 tAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCa                                           <  1:1974467/49‑1 (MQ=255)

                                                 tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa                                       >  1:5577554/1‑53 (MQ=12)

                                                 tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa                                       >  1:770928/1‑53 (MQ=12)

                                                 tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                 <  1:1843464/59‑1 (MQ=255)

                                                 tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc                                 <  1:1008395/59‑1 (MQ=255)

                                                    tgaatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCAc                  <  1:159215/68‑1 (MQ=255)

                                                    tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa                                        <  1:879306/49‑1 (MQ=255)

                                                    tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                  <  1:954640/71‑1 (MQ=255)

                                                      gatgatGTTCAGTGCAGTGATGTACTGTTTagccag                                                   <  1:921637/36‑1 (MQ=255)

                                                      gatgatGTTCAGTGCAGTGATGTACTGTTTagccag                                                   <  1:3014893/36‑1 (MQ=255)

                                                      gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt                                  >  1:1952591/1‑53 (MQ=255)

                                                      gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt                 <  1:2768294/70‑1 (MQ=255)

                                                      aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt                >  1:1423752/2‑71 (MQ=255)

                                                       atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACttt               >  1:4764583/1‑70 (MQ=255)

                                                        tgatgTTCAGTGCAGTGATGTACTGTTTACCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtca              <  1:2401335/71‑1 (MQ=255)

                                                        tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg              >  1:3939805/1‑69 (MQ=255)

                                                        tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg              <  1:1182116/71‑3 (MQ=255)

                                                        tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg              <  1:2684964/71‑3 (MQ=255)

                                                          atgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc                                >  1:4782532/1‑51 (MQ=255)

                                                           tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca           <  1:1855108/71‑1 (MQ=255)

                                                             ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca           >  1:4852286/1‑69 (MQ=255)

                                                             ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa                   <  1:5063728/61‑1 (MQ=255)

                                                              tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg        >  1:4597266/1‑71 (MQ=255)

                                                               cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg        >  1:5662568/1‑70 (MQ=255)

                                                               cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg        >  1:528916/1‑70 (MQ=255)

                                                                 gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc     <  1:5513822/71‑1 (MQ=255)

                                                                 gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc     <  1:3086584/71‑1 (MQ=255)

                                                                 gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc     <  1:1142408/71‑1 (MQ=255)

GTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGG  >  minE/608706‑608844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`