Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 608,922 C→G 26.4% L444L (CTG→CTC)  pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE608,9220CG26.4% 210.4 / 79.1 121L444L (CTG→CTC) pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (46/43);  new base G (18/14);  total (64/57)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 127 total aligned reads displayed.

GGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTA  >  minE/608860‑608992
                                                              |                                                                      
 gTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtt                                                               <  1:65963/71‑1 (MQ=255)
  ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt                                                              <  1:5431602/71‑1 (MQ=255)
  ttCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGttt                                                              <  1:3358694/71‑1 (MQ=255)
    cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                                             <  1:2553028/70‑1 (MQ=255)
    cAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTc                                                            >  1:4130640/1‑71 (MQ=255)
      gACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc                                                          >  1:1343391/1‑71 (MQ=255)
       aCTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                                         <  1:1821597/71‑1 (MQ=255)
         ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                                                    <  1:3575453/58‑1 (MQ=255)
         ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                                                    <  1:2527513/58‑1 (MQ=255)
         ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                                                    <  1:1190732/58‑1 (MQ=255)
         ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCa                                                                    <  1:4165177/58‑1 (MQ=255)
         ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                                                  <  1:1342268/60‑1 (MQ=255)
         ttCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                                                  <  1:4931906/60‑1 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATg                                                                  >  1:4370299/1‑58 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGc                                                          >  1:2451882/1‑66 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                                                      <  1:3010404/70‑1 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAgg                                                      <  1:1832260/70‑1 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg                                                       <  1:808652/69‑1 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     >  1:3961435/1‑71 (MQ=255)
           cGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGt                                                     >  1:4920491/1‑71 (MQ=255)
                cgaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcg                                                >  1:5607572/3‑70 (MQ=255)
                cAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCa                                                >  1:2086234/1‑71 (MQ=255)
                 gaCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgc                                               >  1:4024147/2‑69 (MQ=255)
                  aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGcc                                                         >  1:2503781/1‑60 (MQ=255)
                  aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                                              <  1:2942887/71‑1 (MQ=255)
                  aCCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcg                                              <  1:4610772/71‑1 (MQ=255)
                  aCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcg                                              <  1:62444/71‑1 (MQ=255)
                    cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg                                            >  1:2418760/1‑71 (MQ=255)
                    cTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCAcgcg                                            <  1:3899536/71‑1 (MQ=255)
                    cTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgtg                                            <  1:604767/71‑3 (MQ=255)
                       cATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAc                                         >  1:664257/1‑71 (MQ=255)
                         ttttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCAcc                                        >  1:3300909/1‑70 (MQ=255)
                           ttCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAg                                                                      <  1:3972192/38‑1 (MQ=255)
                            tCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCg                                       >  1:4919861/1‑68 (MQ=255)
                             cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGcgcgtg                                            >  1:5664726/1‑60 (MQ=255)
                             cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag                                    <  1:5057062/70‑1 (MQ=255)
                             cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag                                    <  1:3515728/70‑1 (MQ=255)
                             cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag                                    <  1:5081114/70‑1 (MQ=255)
                             cAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaag                                    <  1:3217052/70‑1 (MQ=255)
                              aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:366141/1‑71 (MQ=255)
                              aGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  <  1:2485728/71‑1 (MQ=255)
                                tttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACt                                <  1:3820837/71‑1 (MQ=255)
                                 ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGtttt                                                             >  1:1781154/1‑41 (MQ=255)
                                 ttttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                               >  1:4708645/1‑71 (MQ=255)
                                 ttctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg                               >  1:5586913/4‑71 (MQ=255)
                                  tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg                                                       >  1:2557281/1‑46 (MQ=255)
                                  tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAg                                                       >  1:42513/1‑46 (MQ=255)
                                  tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt                                                    >  1:3815029/1‑49 (MQ=255)
                                  tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                               >  1:2813492/1‑70 (MQ=255)
                                  tttCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTg                               >  1:3031028/1‑70 (MQ=255)
                                  tttCGTCAACGCCACCGTTGATTGCGTACAGCATGGTTTTCGCCAGGtt                                                    >  1:1673985/1‑49 (MQ=255)
                                   ctCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGc                              <  1:5301396/69‑1 (MQ=255)
                                    tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                            >  1:2057723/1‑71 (MQ=255)
                                    tCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCAt                            >  1:1163239/1‑71 (MQ=255)
                                    tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:4648764/1‑65 (MQ=255)
                                    tCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:4202879/1‑65 (MQ=255)
                                     cGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCAt                            <  1:3273245/70‑1 (MQ=255)
                                       tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg                         <  1:5268998/71‑1 (MQ=255)
                                       tCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTg                         <  1:1694310/71‑1 (MQ=255)
                                         aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       >  1:2669789/1‑71 (MQ=255)
                                         aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       >  1:2402722/1‑71 (MQ=255)
                                         aaCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       <  1:898099/71‑1 (MQ=255)
                                          aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACTCGCACCGAAGAACTGCATTTGtt                       <  1:2118390/70‑1 (MQ=255)
                                          aCGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGtt                       >  1:3459613/1‑70 (MQ=255)
                                           ccccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa                             <  1:1155144/61‑1 (MQ=255)
                                           ccccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa                             <  1:2393552/61‑1 (MQ=255)
                                            cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc                    >  1:1557742/2‑71 (MQ=255)
                                            cccgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAc                    >  1:3756992/2‑71 (MQ=255)
                                             ccgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTAc                    >  1:5557805/1‑70 (MQ=255)
                                              cgccgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaag                                    <  1:4665827/53‑1 (MQ=255)
                                              cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTgct                       >  1:1081659/1‑64 (MQ=255)
                                              cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTgct                       >  1:3426410/1‑64 (MQ=255)
                                              cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                   >  1:4221099/1‑70 (MQ=255)
                                              cgccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                   >  1:5186119/1‑70 (MQ=255)
                                                ccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTa                     >  1:5516307/1‑66 (MQ=255)
                                                ccgTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaat                <  1:2375314/71‑2 (MQ=255)
                                                 cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg               <  1:775996/71‑1 (MQ=255)
                                                 cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg               <  1:2771617/71‑1 (MQ=255)
                                                   ttGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGat             >  1:4718237/1‑71 (MQ=255)
                                                    taaTTGCTTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCa                             <  1:438023/52‑1 (MQ=255)
                                                    tGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaa                                     >  1:3720903/1‑46 (MQ=255)
                                                     gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc            <  1:4320014/70‑1 (MQ=255)
                                                     gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc            <  1:2818379/70‑1 (MQ=255)
                                                     gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc            <  1:4810051/70‑1 (MQ=255)
                                                      aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca           <  1:3114830/70‑1 (MQ=255)
                                                      aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca           <  1:261160/70‑1 (MQ=255)
                                                        tGCGTACAGCATGGTTTTCCCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTa                     >  1:3366110/1‑58 (MQ=255)
                                                         gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcca                                       >  1:4149446/1‑38 (MQ=9)
                                                         gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCAcca                                       >  1:1466690/1‑38 (MQ=9)
                                                         gCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaaagaa                                  >  1:4326718/1‑44 (MQ=12)
                                                         gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg       >  1:5340523/1‑71 (MQ=255)
                                                         gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg       >  1:3865652/1‑71 (MQ=255)
                                                          catACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:2913451/3‑43 (MQ=255)
                                                          cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCgaagaa                                  >  1:2067710/1‑43 (MQ=255)
                                                            tACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt    <  1:4018357/71‑1 (MQ=255)
                                                              cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc                                 >  1:3498844/1‑40 (MQ=255)
                                                              cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAAc                                 >  1:136434/1‑40 (MQ=255)
                                                              cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa  >  1:5663115/1‑71 (MQ=255)
                                                              cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa  >  1:2240311/1‑71 (MQ=255)
                                                              cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa  >  1:5572364/1‑71 (MQ=255)
                                                              |                                                                      
GGTTCAGACTTCGGACCAACCTGCATTTTCAGTTTTTCGTCAACGCCGCCGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTA  >  minE/608860‑608992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑