| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,978 | C→T | 18.4% | V426I (GTT→ATT) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,978 | 0 | C | T | 18.4% | 335.7 / 60.4 | 152 | V426I (GTT→ATT) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base C (46/78); new base T (15/13); total (61/91) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
Only 100 of 155 total aligned reads displayed.
CGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCG > minE/608909‑609048 | cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg < 1:775996/71‑1 (MQ=255)cgTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACg < 1:2771617/71‑1 (MQ=255) ttGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGat > 1:4718237/1‑71 (MQ=255) aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:3114830/70‑1 (MQ=255) aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:261160/70‑1 (MQ=255) gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg > 1:3865652/1‑71 (MQ=255) gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg > 1:5340523/1‑71 (MQ=255) cGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg < 1:493001/70‑1 (MQ=255) cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:5663115/1‑71 (MQ=255) aGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTAc < 1:196137/71‑1 (MQ=255) cATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:4089256/68‑1 (MQ=255) aTGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:3132667/1‑67 (MQ=255) tGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcag > 1:814231/1‑71 (MQ=255) tGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACAATCATCGGGCTTACg < 1:508464/68‑1 (MQ=255) cgTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag > 1:1143248/2‑70 (MQ=255) gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc < 1:1461504/70‑1 (MQ=255) gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc > 1:1569553/1‑70 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGATGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:4923260/63‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:576990/54‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:399597/54‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaa < 1:4812571/71‑1 (MQ=255) tttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:5117703/1‑71 (MQ=255) tttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:5591309/1‑71 (MQ=255) ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:2391923/1‑70 (MQ=255) ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:5207214/1‑68 (MQ=255) ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:5184458/1‑68 (MQ=255) ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:5332640/1‑68 (MQ=255) ttCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagcagg > 1:32789/1‑68 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:2032018/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:202592/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:5547664/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:3043363/70‑1 (MQ=255) tCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc < 1:4334380/70‑1 (MQ=255) cGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagca < 1:2937922/70‑1 (MQ=255) gCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAgcaagcaa < 1:4224293/70‑1 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc > 1:1341037/1‑44 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4240566/44‑1 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc > 1:4642752/1‑64 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg < 1:224252/71‑1 (MQ=255) gTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt < 1:4766030/51‑1 (MQ=255) gTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt < 1:4772410/51‑1 (MQ=255) tttGCACGCGCACCGAAGAACTGCATTTGTTTACCAAc < 1:1089070/38‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:2935523/39‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:5612882/39‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg < 1:2511788/44‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg < 1:4919366/44‑1 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat > 1:4914727/1‑39 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat > 1:827949/1‑39 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:252889/38‑1 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:5496042/38‑1 (MQ=255) gcgcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCg < 1:3806412/71‑1 (MQ=255) gcgcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCg < 1:1110034/71‑1 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgtt > 1:1677563/1‑71 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgtt > 1:3664825/1‑71 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgtt > 1:4528867/1‑71 (MQ=255) gcACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAgcaggcaa < 1:4796127/55‑1 (MQ=255) cACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc < 1:3759162/58‑1 (MQ=255) cACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgttg < 1:2154030/71‑1 (MQ=255) cACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctg > 1:1946567/1‑71 (MQ=255) cACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctg > 1:3185884/1‑71 (MQ=255) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctgtt > 1:514048/3‑70 (MQ=255) cGAAGATCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:2340665/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgca < 1:3427250/43‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg < 1:192719/56‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:4459885/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:3985486/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:2209975/71‑1 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca > 1:4493100/1‑45 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca > 1:2836770/1‑45 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca > 1:2612485/1‑45 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGa < 1:217997/71‑1 (MQ=255) aagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATa > 1:1469284/1‑51 (MQ=255) aagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc < 1:3781532/53‑1 (MQ=255) aagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg > 1:4654861/1‑69 (MQ=255) aagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAAtc > 1:4440321/1‑50 (MQ=255) aagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg > 1:2232396/1‑69 (MQ=255) agaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc < 1:3829407/46‑1 (MQ=16) gaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGt > 1:24494/1‑71 (MQ=255) aaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:5574477/44‑1 (MQ=255) aaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtcgc < 1:3580464/61‑2 (MQ=255) aaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc < 1:1569837/71‑1 (MQ=255) cTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:2567882/64‑1 (MQ=255) cTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGaa > 1:5081927/1‑66 (MQ=255) tGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTc < 1:762743/53‑1 (MQ=255) tGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTc < 1:2918683/53‑1 (MQ=255) gCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAAt > 1:1195933/1‑43 (MQ=255) gCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAAt > 1:515316/1‑43 (MQ=255) cATTTGTTTACCAACGATCATCGGGCTTACGCAgcaagcaa > 1:4831019/1‑41 (MQ=255) aTTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACg < 1:1878427/71‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:5176578/65‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:659474/65‑1 (MQ=255) tGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCAt > 1:268613/1‑71 (MQ=255) gTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:5244188/1‑65 (MQ=255) tttACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgttg < 1:172749/52‑1 (MQ=255) tttACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCAt > 1:4548540/1‑69 (MQ=255) tACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAg < 1:1626106/70‑1 (MQ=255) aCCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAgg < 1:3253912/70‑1 (MQ=255) ccAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGt < 1:1329495/70‑1 (MQ=255) aaCGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt < 1:2096741/71‑1 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:4051844/71‑1 (MQ=255) | CGTTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCG > minE/608909‑609048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |