| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 608,981 | T→C | 21.2% | I425V (ATC→GTC) ‡ | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 608,981 | 0 | T | C | 21.2% | 256.7 / 62.4 | 132 | I425V (ATC→GTC) ‡ | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base T (42/62); new base C (15/13); total (57/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 159 total aligned reads displayed.
TTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTC > minE/608911‑609051 | gATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:2818379/70‑1 (MQ=255) aTTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:261160/70‑1 (MQ=255) gCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCgg > 1:5340523/1‑71 (MQ=255) cAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:2240311/1‑71 (MQ=255) aGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTAc < 1:196137/71‑1 (MQ=255) cATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:4089256/68‑1 (MQ=255) cATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:5202882/68‑1 (MQ=255) cATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACg > 1:2583007/1‑70 (MQ=255) aTGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa > 1:3132667/1‑67 (MQ=255) tGGTTTTCGGCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACg < 1:4912499/68‑1 (MQ=255) tGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcag > 1:814231/1‑71 (MQ=255) tGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACAATCATCGGGCTTACg < 1:508464/68‑1 (MQ=255) cgTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcag > 1:1143248/2‑70 (MQ=255) gTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACgcagc < 1:1461504/70‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGATGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:4923260/63‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:3517010/54‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:576990/54‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCtt < 1:1943784/62‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaa < 1:5109958/71‑1 (MQ=255) ttttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaa < 1:4812571/71‑1 (MQ=255) tttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc > 1:5134384/1‑52 (MQ=255) tttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:3055222/1‑71 (MQ=255) ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:2391923/1‑70 (MQ=255) ttCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaag > 1:2663018/1‑70 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt < 1:5587630/58‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:2032018/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:202592/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:3043363/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:5547664/70‑1 (MQ=255) tCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:5274704/70‑1 (MQ=255) tCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc < 1:4334380/70‑1 (MQ=255) gCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAgcaagcaa < 1:4224293/70‑1 (MQ=255) aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc > 1:945354/1‑71 (MQ=255) aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc > 1:2480333/1‑71 (MQ=255) aGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc > 1:4947648/1‑71 (MQ=255) aGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGc < 1:4923163/71‑1 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc > 1:1341037/1‑44 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:4240566/44‑1 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc > 1:4642752/1‑64 (MQ=255) ggTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg < 1:224252/71‑1 (MQ=255) gTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCt < 1:4766030/51‑1 (MQ=255) gTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGt < 1:2925223/71‑1 (MQ=255) tttGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat < 1:1834487/44‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:5612882/39‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGatc < 1:2935523/39‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTa < 1:3726161/49‑1 (MQ=255) gCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACg > 1:3165380/1‑51 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat > 1:1657053/1‑39 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatcat > 1:4914727/1‑39 (MQ=255) aCGCGCACCGAAGAACTGCATTTGTTTACCAACGatca < 1:252889/38‑1 (MQ=255) gcgcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCg < 1:1110034/71‑1 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgtt > 1:4528867/1‑71 (MQ=255) gcACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAgcaggcaa < 1:4796127/55‑1 (MQ=255) cACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc < 1:4355125/58‑1 (MQ=255) cACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGc < 1:3759162/58‑1 (MQ=255) cACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgttg < 1:2154030/71‑1 (MQ=255) cACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAAtc < 1:4774095/56‑2 (MQ=255) aCCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc < 1:5172893/47‑1 (MQ=16) aCCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc < 1:2560571/47‑1 (MQ=16) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctgtt > 1:514048/3‑70 (MQ=255) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg < 1:3782391/69‑1 (MQ=255) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg < 1:5360228/69‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCg < 1:192719/56‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:3985486/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:4459885/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:2209975/71‑1 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca > 1:4493100/1‑45 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACgcagca > 1:2836770/1‑45 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGa < 1:217997/71‑1 (MQ=255) aaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg > 1:1616873/2‑70 (MQ=255) aagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATa > 1:4730602/1‑51 (MQ=255) aagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg > 1:4654861/1‑69 (MQ=255) aagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACgcagc > 1:1492323/1‑43 (MQ=16) agaaCTGCATTTGTTTACCAACGATCATCGGGCtt < 1:3276428/35‑1 (MQ=255) agaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc < 1:3828394/46‑1 (MQ=16) agaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCagcaggc < 1:3829407/46‑1 (MQ=16) aaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCagcaagc < 1:5574477/44‑1 (MQ=255) aaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtcgc < 1:3580464/61‑2 (MQ=255) aaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc < 1:1569837/71‑1 (MQ=255) cTGCATTTGTTTACCAACGATCATCGGGCTTACGCAgcaagcaa > 1:5648645/1‑44 (MQ=255) tGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTc < 1:762743/53‑1 (MQ=255) gCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAAt > 1:1195933/1‑43 (MQ=255) tttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATa < 1:2369170/41‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTc < 1:3881398/48‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:5176578/65‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:659474/65‑1 (MQ=255) gTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:5244188/1‑65 (MQ=255) tttACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgttg < 1:172749/52‑1 (MQ=255) tttACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCAt > 1:4548540/1‑69 (MQ=255) aCCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAgg < 1:3253912/70‑1 (MQ=255) ccAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGt < 1:1329495/70‑1 (MQ=255) aaCGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt < 1:2096741/71‑1 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg > 1:822877/1‑71 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:4051844/71‑1 (MQ=255) gATCATCGGTCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGt > 1:3346541/1‑71 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:1007848/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:4006540/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:1557215/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc < 1:3016969/71‑1 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc < 1:2113837/71‑1 (MQ=255) | TTGATTGCGTACAGCATGGTTTTCGCCAGGTTTGCACGCGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTC > minE/608911‑609051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |