| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,019 | T→C | 17.6% | N412S (AAC→AGC) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,019 | 0 | T | C | 17.6% | 268.9 / 47.5 | 125 | N412S (AAC→AGC) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base T (43/60); new base C (12/10); total (55/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
Only 100 of 150 total aligned reads displayed.
CGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAG > minE/608949‑609088 | cgcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgt < 1:1980355/71‑1 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgtt > 1:3664825/1‑71 (MQ=255) gcACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgtt > 1:1677563/1‑71 (MQ=255) cACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCgttg < 1:2154030/71‑1 (MQ=255) cACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctg > 1:1946567/1‑71 (MQ=255) cACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctg > 1:3185884/1‑71 (MQ=255) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCgctgtt > 1:514048/3‑70 (MQ=255) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg < 1:5360228/69‑1 (MQ=255) caaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg < 1:3782391/69‑1 (MQ=255) cGAAGATCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:2340665/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:4459885/71‑1 (MQ=255) cGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGttgttg < 1:3985486/71‑1 (MQ=255) gaagaaCTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGa < 1:217997/71‑1 (MQ=255) aaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg > 1:1616873/2‑70 (MQ=255) aaagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg > 1:4544652/2‑70 (MQ=255) aagaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGctgttg > 1:2232396/1‑69 (MQ=255) gaaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGt > 1:24494/1‑71 (MQ=255) aaCTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTc < 1:1569837/71‑1 (MQ=255) aTTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACg < 1:1878427/71‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:5176578/65‑1 (MQ=255) ttGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:659474/65‑1 (MQ=255) tGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCAt > 1:268613/1‑71 (MQ=255) aCCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAgg < 1:3253912/70‑1 (MQ=255) aaCGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt < 1:2096741/71‑1 (MQ=255) gATCATCGGTCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGt > 1:3346541/1‑71 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:4006540/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:1007848/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:947131/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:1557215/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc < 1:3016969/71‑1 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:5542797/1‑71 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:1482523/1‑71 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:4750025/1‑71 (MQ=255) atcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAg < 1:2042020/59‑1 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAg < 1:5126323/56‑1 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:5684195/3‑71 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:4556021/3‑71 (MQ=255) tcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCg < 1:2755966/48‑1 (MQ=255) gggCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCAt < 1:4952891/70‑1 (MQ=255) gggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTca > 1:642556/1‑44 (MQ=255) gggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTca > 1:1596959/1‑44 (MQ=255) ttACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTg < 1:2953341/70‑1 (MQ=255) aCGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGt < 1:3564746/37‑1 (MQ=255) aCGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTg > 1:4535093/1‑68 (MQ=255) gcagcaGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCaagg < 1:4350307/71‑4 (MQ=255) gcagcaGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCaagg < 1:3895082/71‑4 (MQ=255) cagcaAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAgaga < 1:2118396/71‑1 (MQ=255) agcaagcaATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:1982035/1‑38 (MQ=255) agcaagcaATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:3094628/1‑38 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:553177/58‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:1710426/58‑1 (MQ=255) caagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:154232/66‑1 (MQ=255) caagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:312338/66‑1 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:3000644/1‑35 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:1423125/1‑35 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:1216229/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:3710429/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag > 1:3078264/1‑71 (MQ=255) ggcaaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:905889/2‑55 (MQ=255) agcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag < 1:3330683/70‑1 (MQ=255) gcaaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGgt < 1:4835971/71‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt < 1:1435471/71‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt > 1:4337394/1‑71 (MQ=255) caaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATc > 1:41501/1‑43 (MQ=12) aaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGgtga < 1:4618897/71‑2 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCa < 1:2824134/37‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCa < 1:4323708/42‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:5588921/71‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:1082422/71‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc > 1:2302400/1‑71 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:1385924/50‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:1803017/59‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:1820379/59‑1 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAg > 1:3662086/1‑35 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:1150438/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:4247255/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:5055751/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc > 1:2581567/1‑45 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGAt < 1:2751295/71‑1 (MQ=255) gCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGa < 1:940828/70‑1 (MQ=255) gCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGAt > 1:2137280/1‑71 (MQ=255) tAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGa < 1:3301044/71‑1 (MQ=255) aGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATAc < 1:5126632/47‑1 (MQ=255) aGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATAc < 1:2223316/47‑1 (MQ=255) gTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCAt < 1:2293534/44‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:5290414/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:3235701/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:235236/40‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCagag < 1:5667610/52‑1 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATa > 1:770698/1‑42 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCa < 1:4137198/47‑1 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3179307/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3379225/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:705335/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:445539/1‑71 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:2456467/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:5640415/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:1334793/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTAATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3116851/1‑70 (MQ=255) gttgttGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTa < 1:845802/70‑1 (MQ=255) | CGCACCGAAGAACTGCATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAG > minE/608949‑609088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |