| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,032 | G→T | 14.3% | P408T (CCG→ACG) ‡ | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,032 | 0 | G | T | 14.3% | 365.4 / 28.5 | 125 | P408T (CCG→ACG) ‡ | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (45/62); new base T (9/9); total (54/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
ATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTG > minE/608965‑609102 | tGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCAt > 1:268613/1‑71 (MQ=255) gTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:5244188/1‑65 (MQ=255) tttACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCAt > 1:4548540/1‑69 (MQ=255) tACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAg < 1:1626106/70‑1 (MQ=255) aCCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAgg < 1:3253912/70‑1 (MQ=255) ccAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGt < 1:1329495/70‑1 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg > 1:822877/1‑71 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:4051844/71‑1 (MQ=255) gATCATCGGTCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGt > 1:3346541/1‑71 (MQ=255) accattGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtt < 1:2958484/69‑1 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:1007848/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:4006540/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:1557215/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc > 1:947131/1‑64 (MQ=255) tcatcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc < 1:2113837/71‑1 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:5542797/1‑71 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:1482523/1‑71 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:4750025/1‑71 (MQ=255) atcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAg < 1:2042020/59‑1 (MQ=255) atcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:439156/70‑1 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAg < 1:5126323/56‑1 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:3290511/3‑71 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:5684195/3‑71 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:4556021/3‑71 (MQ=255) gggCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCAt < 1:4952891/70‑1 (MQ=255) gggCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATa > 1:230515/1‑71 (MQ=255) cGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:2738835/71‑1 (MQ=255) gcagcaGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCaagg < 1:4350307/71‑4 (MQ=255) gcagcaGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCaagg < 1:3895082/71‑4 (MQ=255) gcagcaAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTc < 1:373776/51‑1 (MQ=255) cagcaAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAgaga < 1:2118396/71‑1 (MQ=255) agcaagcaATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:3094628/1‑38 (MQ=255) agcaagcaATAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:1982035/1‑38 (MQ=255) agcaagcaATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGATCTCATACTGCAGagaa < 1:1570117/71‑1 (MQ=255) gcaggcaaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAggaag < 1:2366258/71‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:553177/58‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:564540/58‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:2007039/71‑1 (MQ=255) caagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:312338/66‑1 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:4328136/1‑35 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGa > 1:3000644/1‑35 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:3710429/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:515667/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:1216229/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag > 1:3078264/1‑71 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag < 1:316184/71‑1 (MQ=255) gcaaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGgt < 1:4835971/71‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt < 1:1435471/71‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt < 1:1461097/71‑1 (MQ=255) caaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATc > 1:41501/1‑43 (MQ=12) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCa < 1:2824134/37‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCa < 1:4323708/42‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:5588921/71‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:1082422/71‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:1551045/71‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:1385924/50‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:1803017/59‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:1820379/59‑1 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAg > 1:3662086/1‑35 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:1150438/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:4247255/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGtt > 1:938943/1‑44 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc > 1:2581567/1‑45 (MQ=255) gCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGa < 1:940828/70‑1 (MQ=255) gCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGAt > 1:2137280/1‑71 (MQ=255) tAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGt < 1:1850512/40‑1 (MQ=255) tAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGa < 1:3301044/71‑1 (MQ=255) aGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATAc < 1:2223316/47‑1 (MQ=255) aGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATAc < 1:5126632/47‑1 (MQ=255) gTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCAt < 1:2293534/44‑1 (MQ=255) tcgtCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGacac < 1:1115463/68‑1 (MQ=255) tcgtCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGa > 1:1970741/4‑68 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:5290414/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:4903047/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:3235701/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:235236/40‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:3550239/40‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCagag < 1:5667610/52‑1 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATa > 1:770698/1‑42 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCa < 1:4137198/47‑1 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3379225/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:445539/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3179307/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:705335/1‑71 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:2456467/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:1334793/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTAATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3110437/1‑70 (MQ=255) cGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:2280545/51‑1 (MQ=255) cGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:2781174/1‑69 (MQ=255) gttgttGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTa < 1:845802/70‑1 (MQ=255) gttgttGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAg > 1:5548385/1‑71 (MQ=255) tgttgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAgcg > 1:456444/1‑71 (MQ=255) ttgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaaga < 1:5177378/47‑1 (MQ=255) tgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCagag < 1:2901027/42‑1 (MQ=255) tgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCagag < 1:4411910/42‑1 (MQ=255) gAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAgg > 1:2996215/1‑47 (MQ=255) aaGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:2942945/1‑42 (MQ=255) cGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTc < 1:5337889/70‑1 (MQ=255) cGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTc < 1:2198931/70‑1 (MQ=255) gACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg < 1:3077275/71‑1 (MQ=255) | ATTTGTTTACCAACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTG > minE/608965‑609102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |