| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,045 | A→G | 16.3% | D403D (GAT→GAC) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,045 | 0 | A | G | 16.3% | 294.0 / 43.7 | 129 | D403D (GAT→GAC) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base A (41/67); new base G (11/10); total (52/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
AACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGC > minE/608976‑609114 | aaCGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt < 1:2096741/71‑1 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg > 1:822877/1‑71 (MQ=255) cGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:4051844/71‑1 (MQ=255) accattGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtt < 1:2958484/69‑1 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:4750025/1‑71 (MQ=255) catcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct > 1:5542797/1‑71 (MQ=255) atcGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:439156/70‑1 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:3290511/3‑71 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:4556021/3‑71 (MQ=255) ttgggCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:5684195/3‑71 (MQ=255) gggCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCAt < 1:4952891/70‑1 (MQ=255) gggCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATa > 1:230515/1‑71 (MQ=255) ttACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTg < 1:2953341/70‑1 (MQ=255) aCGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTg > 1:4535093/1‑68 (MQ=255) cGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:2738835/71‑1 (MQ=255) gcagcaGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCaagg < 1:4350307/71‑4 (MQ=255) cagcaAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAgaga < 1:2118396/71‑1 (MQ=255) agcaagcaATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGATCTCATACTGCAGagaa < 1:1570117/71‑1 (MQ=255) gcaggcaaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAggaag < 1:2366258/71‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:564540/58‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:553177/58‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:1710426/58‑1 (MQ=255) gcaagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:3215043/58‑1 (MQ=255) caagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:154232/66‑1 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:515667/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:3710429/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaa > 1:1216229/1‑68 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag < 1:316184/71‑1 (MQ=255) aagcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag > 1:3078264/1‑71 (MQ=255) ggcaaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCa > 1:905889/2‑55 (MQ=255) agcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAagag < 1:3330683/70‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt < 1:419717/71‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt < 1:1461097/71‑1 (MQ=255) gcaaTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGgt < 1:1435471/71‑1 (MQ=255) aaTCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGgtga < 1:4618897/71‑2 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCa < 1:4323708/42‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:1082422/71‑1 (MQ=255) aTAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:1551045/71‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCa < 1:1385924/50‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCaga < 1:1820379/59‑1 (MQ=255) tAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCg < 1:5383566/71‑1 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:5055751/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:1150438/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCAt > 1:4247255/1‑40 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGtt > 1:938943/1‑44 (MQ=255) gCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtc > 1:2581567/1‑45 (MQ=255) gCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGa < 1:940828/70‑1 (MQ=255) gCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGAt > 1:2137280/1‑71 (MQ=255) tAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGt < 1:1850512/40‑1 (MQ=255) tAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGa < 1:3301044/71‑1 (MQ=255) aGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATAc < 1:2223316/47‑1 (MQ=255) gTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCAt < 1:2293534/44‑1 (MQ=255) tcgtCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGacac < 1:1115463/68‑1 (MQ=255) tcgtCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGa > 1:1970741/4‑68 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:5290414/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCg < 1:4903047/36‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:235236/40‑1 (MQ=255) tCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTtct < 1:3550239/40‑1 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATa > 1:770698/1‑42 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCa < 1:4137198/47‑1 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3379225/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3179307/1‑71 (MQ=255) aTCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:705335/1‑71 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:5640415/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:1334793/52‑1 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTAATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3110437/1‑70 (MQ=255) tCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTAATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3116851/1‑70 (MQ=255) cGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaag < 1:2280545/51‑1 (MQ=255) cGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:2781174/1‑69 (MQ=255) gttgttGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAg > 1:5548385/1‑71 (MQ=255) ttgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGagaaga < 1:5177378/47‑1 (MQ=255) tgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCagag < 1:4411910/42‑1 (MQ=255) tgAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCagag < 1:2901027/42‑1 (MQ=255) gAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAgg > 1:2996215/1‑47 (MQ=255) cGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTc < 1:2198931/70‑1 (MQ=255) cGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTc < 1:5337889/70‑1 (MQ=255) gACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg < 1:3077275/71‑1 (MQ=255) gACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGAAACTTTAGCGGCGAATTTCtt > 1:249047/1‑70 (MQ=255) aCGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGa < 1:3909367/71‑1 (MQ=255) cGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg > 1:1036772/1‑69 (MQ=255) cGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTg > 1:5175063/1‑69 (MQ=255) cGCATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGa < 1:1731496/70‑1 (MQ=255) cATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAgcg > 1:1500114/1‑55 (MQ=255) cATCAGATCGTCATTTTCATACTGCAAGGAAGAGGTGACGATCGACACCTGCGCGGCATATTTTTTGAAgg < 1:1224327/71‑2 (MQ=255) aTCAGATCGTCGTTTTCATACTGCAAAGAGGATGTGACGATAGACACCTGCGCGGCATATTTTTTGaa > 1:3717520/1‑68 (MQ=255) tCAGTTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc < 1:3847743/71‑1 (MQ=255) tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:611434/37‑1 (MQ=255) tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:16782/37‑1 (MQ=255) tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTg < 1:2152486/65‑1 (MQ=255) tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc < 1:2073074/71‑1 (MQ=255) tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc < 1:4503201/71‑1 (MQ=255) tCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTc < 1:4737692/71‑1 (MQ=255) cAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGtt > 1:4818782/1‑69 (MQ=255) aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTc < 1:3710638/35‑1 (MQ=255) aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGa > 1:5544640/1‑37 (MQ=255) aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGaa > 1:2111126/1‑65 (MQ=255) aGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGa > 1:2873580/1‑64 (MQ=255) gTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTa < 1:5636241/46‑1 (MQ=255) cATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACtt > 1:3452629/1‑42 (MQ=255) cATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCgg > 1:5694392/1‑70 (MQ=255) | AACGATCATCGGGCTTACGCAGCAAGCAATAGCGTAGTCATCGTTGTTGAAGTCCGGACGCATCAGGTCATCGTTCTCATACTGCAGAGAAGAGGTGTCGATGGACACTTTAGCGGCGAATTTCTTGAAGTTCAGCGGC > minE/608976‑609114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |