| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,210 | G→C | 27.2% | T348T (ACC→ACG) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,210 | 0 | G | C | 27.2% | 133.9 / 55.2 | 81 | T348T (ACC→ACG) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (34/25); new base C (14/8); total (48/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01 | |||||||||||
GTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAT > minE/609141‑609280 | gTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAggg > 1:1284140/1‑71 (MQ=255) ttCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAggg > 1:675934/1‑70 (MQ=255) tCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTa > 1:251769/1‑71 (MQ=255) tCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTa > 1:359129/1‑71 (MQ=255) cGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTAc > 1:5628799/1‑71 (MQ=255) ccGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCg > 1:3917869/1‑71 (MQ=255) cGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCg < 1:4329675/70‑1 (MQ=255) ggAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTc < 1:5267283/71‑1 (MQ=255) ggAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTc < 1:4430501/71‑1 (MQ=255) gaCGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAg < 1:1688737/71‑1 (MQ=255) ggccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:88760/4‑71 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAgg > 1:3280802/1‑54 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc > 1:4277363/1‑71 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:4683451/71‑1 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:831753/71‑1 (MQ=255) gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:5585024/3‑70 (MQ=255) gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:1969608/3‑70 (MQ=255) cccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAt > 1:2876034/1‑71 (MQ=255) ccATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGa > 1:3538263/1‑63 (MQ=255) ccATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGa > 1:1727957/1‑63 (MQ=255) cATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac < 1:1498445/71‑1 (MQ=255) cATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAtcc < 1:28585/71‑3 (MQ=255) aTGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac > 1:3725049/1‑70 (MQ=255) tGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATacca > 1:1355781/1‑71 (MQ=255) gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATcccgcc > 1:3131886/1‑71 (MQ=255) gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATcccgc > 1:4181715/1‑68 (MQ=255) gtgtACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATaccac > 1:1664215/1‑70 (MQ=255) tACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGAt < 1:2213120/71‑1 (MQ=255) cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa > 1:1558318/1‑68 (MQ=255) cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATa < 1:4373535/70‑1 (MQ=255) gggTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATAACACCGATAGAt < 1:4219930/71‑1 (MQ=255) ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:1232033/50‑1 (MQ=255) ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:2075826/50‑1 (MQ=255) ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGt > 1:146097/1‑71 (MQ=255) cAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac > 1:1681998/1‑53 (MQ=255) aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:1815458/70‑1 (MQ=255) aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGc < 1:3223435/71‑1 (MQ=255) aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCAt > 1:180679/1‑46 (MQ=255) aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAg < 1:5120824/71‑1 (MQ=255) aCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGa < 1:1618778/71‑1 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCg > 1:254371/1‑51 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa > 1:1655566/1‑52 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa > 1:3229867/1‑52 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc > 1:2858751/1‑66 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGAt < 1:1468218/71‑1 (MQ=255) ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAt > 1:2928662/1‑52 (MQ=16) ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAt > 1:3848782/1‑52 (MQ=16) ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCAt > 1:3627170/1‑43 (MQ=255) ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATa > 1:2470007/1‑44 (MQ=255) ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc > 1:4980736/1‑65 (MQ=255) aGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg < 1:2476638/70‑1 (MQ=255) ggagTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGt < 1:2964678/68‑1 (MQ=255) gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:1750265/1‑45 (MQ=16) gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:5579429/1‑45 (MQ=16) gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:417144/1‑45 (MQ=16) gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca > 1:2007762/1‑45 (MQ=16) gTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCgg > 1:4654558/1‑53 (MQ=255) tttttGGTCACCAGCGTACGACCGTCCAGCCCCATcccgc < 1:2577542/40‑3 (MQ=12) tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtc < 1:3875433/45‑2 (MQ=12) tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTggg > 1:4588029/1‑66 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:1202690/36‑1 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:3431981/36‑1 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:3161205/36‑1 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:1735616/36‑1 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa < 1:1174576/36‑1 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc < 1:151642/70‑1 (MQ=255) ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc > 1:1515688/1‑69 (MQ=255) ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc > 1:2138134/1‑69 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATacca > 1:1993855/1‑37 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATacc > 1:266622/1‑36 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCg > 1:2000489/1‑62 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc > 1:2712682/1‑69 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc > 1:2474570/1‑69 (MQ=255) ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg < 1:1269378/70‑3 (MQ=255) ggTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCagag > 1:1969865/1‑70 (MQ=255) gtcaCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa < 1:4104932/68‑1 (MQ=255) gTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga < 1:2512797/70‑1 (MQ=255) aCCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg > 1:845085/1‑71 (MQ=255) aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGcc < 1:765651/62‑1 (MQ=255) aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAg < 1:3508966/71‑1 (MQ=255) cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGtt > 1:4221212/1‑71 (MQ=255) cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt > 1:2647042/1‑70 (MQ=255) cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt > 1:3660695/1‑70 (MQ=255) cAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg > 1:2796722/1‑60 (MQ=255) ggTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAt < 1:1293650/71‑1 (MQ=255) | GTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAT > minE/609141‑609280 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |