| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,240 | A→C | 9.7% | S338S (TCT→TCG) ‡ | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,240 | 0 | A | C | 9.7% | 173.3 / 12.1 | 72 | S338S (TCT→TCG) ‡ | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base A (32/33); new base C (2/5); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCA > minE/609171‑609310 | cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATa < 1:4373535/70‑1 (MQ=255) gggTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATAACACCGATAGAt < 1:4219930/71‑1 (MQ=255) ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGt > 1:146097/1‑71 (MQ=255) aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:1815458/70‑1 (MQ=255) aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGc < 1:3223435/71‑1 (MQ=255) aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAg < 1:5120824/71‑1 (MQ=255) aCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGa < 1:1618778/71‑1 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc > 1:2858751/1‑66 (MQ=255) cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGAt < 1:1468218/71‑1 (MQ=255) ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc > 1:4980736/1‑65 (MQ=255) aGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg < 1:2476638/70‑1 (MQ=255) ggagTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGt < 1:2964678/68‑1 (MQ=255) gTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCgg > 1:4654558/1‑53 (MQ=255) tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTggg > 1:4588029/1‑66 (MQ=255) tttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc < 1:151642/70‑1 (MQ=255) ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc > 1:1515688/1‑69 (MQ=255) ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc > 1:2138134/1‑69 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCg > 1:2000489/1‑62 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc > 1:2712682/1‑69 (MQ=255) tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc > 1:2474570/1‑69 (MQ=255) ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg < 1:1269378/70‑3 (MQ=255) ggTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCagag > 1:1969865/1‑70 (MQ=255) gtcaCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa < 1:4104932/68‑1 (MQ=255) gTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga < 1:2512797/70‑1 (MQ=255) aCCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg > 1:845085/1‑71 (MQ=255) aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGcc < 1:765651/62‑1 (MQ=255) aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAg < 1:3508966/71‑1 (MQ=255) cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGtt > 1:4221212/1‑71 (MQ=255) cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt > 1:2647042/1‑70 (MQ=255) cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt > 1:3660695/1‑70 (MQ=255) cAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg > 1:2796722/1‑60 (MQ=255) ggTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAt < 1:1293650/71‑1 (MQ=255) tACGACCGTCGAGGCCCATACCACCGATAGATTCgg > 1:4678409/1‑36 (MQ=255) tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg < 1:2079290/71‑1 (MQ=255) tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg < 1:3889893/71‑1 (MQ=255) cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:3607041/37‑1 (MQ=255) cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg < 1:3613668/37‑1 (MQ=255) aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAtt > 1:1698968/1‑71 (MQ=255) aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAt > 1:624818/1‑70 (MQ=255) ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc > 1:572776/1‑71 (MQ=255) ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc > 1:4124624/1‑71 (MQ=255) gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg < 1:2518763/42‑1 (MQ=255) gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg < 1:850734/71‑1 (MQ=255) gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg < 1:1691527/71‑1 (MQ=255) tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:4986670/55‑1 (MQ=255) tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:3899602/55‑1 (MQ=255) tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:3307660/55‑1 (MQ=255) tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa < 1:4247526/55‑1 (MQ=255) tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg > 1:4205076/1‑70 (MQ=255) aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg > 1:4514107/1‑70 (MQ=255) ccATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTGCGGAGTACg > 1:3484677/1‑70 (MQ=255) cATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc < 1:3358088/46‑1 (MQ=255) cATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc < 1:2868013/46‑1 (MQ=255) cATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc < 1:4291754/46‑1 (MQ=255) aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc < 1:4092524/45‑1 (MQ=255) aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAg > 1:4629968/1‑71 (MQ=255) tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAAc > 1:2804630/1‑44 (MQ=255) tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg > 1:797551/1‑46 (MQ=255) tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg < 1:1712763/71‑1 (MQ=255) tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg < 1:3820930/71‑1 (MQ=255) ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa < 1:821195/70‑1 (MQ=255) ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa < 1:3361070/70‑1 (MQ=255) accGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg > 1:378396/1‑56 (MQ=255) ccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc > 1:5616054/1‑53 (MQ=255) cGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga > 1:2719212/1‑36 (MQ=255) cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGaa > 1:4196245/1‑37 (MQ=255) cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg < 1:1657633/70‑1 (MQ=255) cGATAGATTCGGTTGCCCAGATCGGGTAGCCAGAGaa > 1:2396641/1‑37 (MQ=255) gATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATc > 1:503729/1‑45 (MQ=255) aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTc < 1:5490046/41‑1 (MQ=255) aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc > 1:4952182/1‑71 (MQ=255) tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc < 1:4114301/49‑1 (MQ=255) tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa < 1:2988351/68‑1 (MQ=255) aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGc > 1:1460356/1‑65 (MQ=255) aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc > 1:5282517/1‑70 (MQ=255) aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc > 1:4756260/1‑69 (MQ=255) aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa > 1:399609/1‑71 (MQ=255) | CAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCA > minE/609171‑609310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |