Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 609,283 T→A 6.2% Y324F (TAC→TTC) ‡ pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE609,2830TA 6.2% 278.1 / 7.9 97Y324F (TAC→TTC) ‡pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (61/30);  new base A (4/2);  total (65/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

ACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTT  >  minE/609216‑609352
                                                                   |                                                                     
aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAtt                                                                    >  1:1698968/1‑71 (MQ=255)
aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAt                                                                     >  1:624818/1‑70 (MQ=255)
 ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   >  1:4124624/1‑71 (MQ=255)
 ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   >  1:572776/1‑71 (MQ=255)
   gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 <  1:850734/71‑1 (MQ=255)
   gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 <  1:1691527/71‑1 (MQ=255)
    tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 >  1:4205076/1‑70 (MQ=255)
       aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg                                                              >  1:4514107/1‑70 (MQ=255)
           ccATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTGCGGAGTACg                                                          >  1:3484677/1‑70 (MQ=255)
             aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAg                                                       >  1:4629968/1‑71 (MQ=255)
              tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg                                                      <  1:3820930/71‑1 (MQ=255)
              tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg                                                      <  1:1712763/71‑1 (MQ=255)
                ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa                                                     <  1:3361070/70‑1 (MQ=255)
                ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa                                                     <  1:821195/70‑1 (MQ=255)
                  accGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 >  1:378396/1‑56 (MQ=255)
                   ccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   >  1:5616054/1‑53 (MQ=255)
                    cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:1657633/70‑1 (MQ=255)
                      aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc                                              >  1:4952182/1‑71 (MQ=255)
                       tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   <  1:4114301/49‑1 (MQ=255)
                       tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa                                                <  1:2988351/68‑1 (MQ=255)
                        aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGc                                                  >  1:1460356/1‑65 (MQ=255)
                        aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:5282517/1‑70 (MQ=255)
                        aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc                                              >  1:4756260/1‑69 (MQ=255)
                        aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                            >  1:399609/1‑71 (MQ=255)
                         gATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                            <  1:4702818/70‑1 (MQ=255)
                         acttCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCa                                            >  1:5649674/3‑70 (MQ=255)
                             cGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACg                                        >  1:4007674/1‑70 (MQ=255)
                                  gCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGtt                                   >  1:1803539/1‑70 (MQ=255)
                                   cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                 <  1:5186223/71‑1 (MQ=255)
                                   cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                 <  1:3545860/71‑1 (MQ=255)
                                    ccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:3512965/1‑71 (MQ=255)
                                     cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                               <  1:2266684/71‑1 (MQ=255)
                                     cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                               <  1:3130415/71‑1 (MQ=255)
                                     cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:42153/53‑1 (MQ=255)
                                     cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:4897229/53‑1 (MQ=255)
                                     cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:3613448/53‑1 (MQ=255)
                                      aGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                               >  1:3455217/1‑70 (MQ=255)
                                      aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:123839/1‑70 (MQ=255)
                                      aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:4891136/1‑70 (MQ=255)
                                      aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:3794498/1‑70 (MQ=255)
                                      aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:3589419/1‑70 (MQ=255)
                                      aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:2193704/1‑70 (MQ=255)
                                      aGATCGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:4897799/1‑70 (MQ=255)
                                       gATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                             >  1:3407185/1‑71 (MQ=255)
                                       gATCGGGTCGCCAGAGAACAGTTCATCGTATTTCGGAGTACGCAGGAAGCGa                                                >  1:190192/1‑52 (MQ=255)
                                       gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGc                                                         <  1:2008814/43‑1 (MQ=255)
                                       gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                               >  1:5165173/1‑53 (MQ=255)
                                       gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                               >  1:2798119/1‑53 (MQ=255)
                                       gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                               >  1:4145257/1‑53 (MQ=255)
                                        aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGa                                                               >  1:5447180/1‑36 (MQ=255)
                                        aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGa                                                               >  1:440374/1‑36 (MQ=255)
                                        aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                            <  1:3607582/71‑1 (MQ=255)
                                         ttgggTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                             >  1:1024587/3‑69 (MQ=255)
                                         tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCa                                                        >  1:352507/1‑42 (MQ=255)
                                         tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa                                                >  1:1816350/1‑50 (MQ=255)
                                         tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                          >  1:3601350/1‑56 (MQ=255)
                                         tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                          >  1:23699/1‑56 (MQ=255)
                                         tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                           <  1:3888221/71‑1 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:5177806/1‑52 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:3362506/1‑52 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:5637492/1‑52 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:2447193/1‑52 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  >  1:722803/1‑63 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:5626028/1‑65 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                           <  1:2468755/70‑1 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                           <  1:5527717/70‑1 (MQ=255)
                                          cGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCg                                                 >  1:5453611/1‑48 (MQ=255)
                                            ggTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                            <  1:2814762/51‑1 (MQ=255)
                                               cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtg                      >  1:4023666/1‑70 (MQ=255)
                                                gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 >  1:3567643/1‑42 (MQ=255)
                                                gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                            >  1:4516847/1‑63 (MQ=255)
                                                gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                    >  1:3271539/1‑71 (MQ=255)
                                                gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                    >  1:5303577/1‑71 (MQ=255)
                                                  cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                  <  1:5031927/71‑1 (MQ=255)
                                                  cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGt                                    <  1:228596/53‑1 (MQ=255)
                                                  cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                  <  1:278814/71‑1 (MQ=255)
                                                   agagAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAg                         >  1:1972603/1‑63 (MQ=255)
                                                    gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                          <  1:4348556/45‑1 (MQ=255)
                                                    gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                 >  1:2931471/1‑69 (MQ=255)
                                                    gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                 >  1:878281/1‑69 (MQ=255)
                                                      gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa              <  1:1885619/71‑1 (MQ=255)
                                                        aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt            >  1:1719359/1‑71 (MQ=255)
                                                        aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt            >  1:4089161/1‑71 (MQ=255)
                                                         cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt            >  1:2999037/1‑70 (MQ=255)
                                                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:4923374/45‑1 (MQ=255)
                                                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:2220146/45‑1 (MQ=255)
                                                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:4537443/45‑1 (MQ=255)
                                                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:64744/45‑1 (MQ=255)
                                                             tCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAg                                     >  1:4645613/1‑41 (MQ=255)
                                                             tCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAg                                     >  1:2543439/1‑41 (MQ=255)
                                                              cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:2829690/1‑45 (MQ=255)
                                                              cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:5633186/1‑45 (MQ=255)
                                                              cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac      >  1:3684893/1‑69 (MQ=255)
                                                              cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc      >  1:3693472/1‑71 (MQ=255)
                                                              cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc      >  1:1981534/1‑71 (MQ=255)
                                                              cATCGTATTCCGGAGTAAGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:5174622/1‑45 (MQ=255)
                                                                  gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt  >  1:4107251/1‑71 (MQ=255)
                                                                   |                                                                     
ACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTT  >  minE/609216‑609352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: