Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,291	A→T	10.0%	T321T (ACT→ACA)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,291	0	A	T	10.0%	264.8 / 15.7	98	T321T (ACT→ACA)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (55/33);  new base T (7/3);  total (62/36)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTT  >  minE/609223‑609358                                                                     |                                                                    aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg                                                                    >  1:4514107/1‑70 (MQ=255)     ccATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTGCGGAGTACg                                                                >  1:3484677/1‑70 (MQ=255)       aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAg                                                             >  1:4629968/1‑71 (MQ=255)        tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg                                                            <  1:3820930/71‑1 (MQ=255)        tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg                                                            <  1:1712763/71‑1 (MQ=255)          ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa                                                           <  1:821195/70‑1 (MQ=255)          ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa                                                           <  1:3361070/70‑1 (MQ=255)              cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                       <  1:1657633/70‑1 (MQ=255)                aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc                                                    >  1:4952182/1‑71 (MQ=255)                 tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa                                                      <  1:2988351/68‑1 (MQ=255)                  aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGc                                                        >  1:1460356/1‑65 (MQ=255)                  aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                                   >  1:5282517/1‑70 (MQ=255)                  aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc                                                    >  1:4756260/1‑69 (MQ=255)                  aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                                  >  1:399609/1‑71 (MQ=255)                   gATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                                  <  1:4702818/70‑1 (MQ=255)                   acttCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCa                                                  >  1:5649674/3‑70 (MQ=255)                       cGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACg                                              >  1:4007674/1‑70 (MQ=255)                            gCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGtt                                         >  1:1803539/1‑70 (MQ=255)                             cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                       <  1:3545860/71‑1 (MQ=255)                             cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                       <  1:5186223/71‑1 (MQ=255)                              ccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                      >  1:3512965/1‑71 (MQ=255)                               cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                                     <  1:2266684/71‑1 (MQ=255)                               cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                                     <  1:3130415/71‑1 (MQ=255)                               cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                       <  1:42153/53‑1 (MQ=255)                               cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                       <  1:3613448/53‑1 (MQ=255)                               cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                       <  1:4897229/53‑1 (MQ=255)                                aGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                                     >  1:3455217/1‑70 (MQ=255)                                aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                     >  1:4891136/1‑70 (MQ=255)                                aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                     >  1:3794498/1‑70 (MQ=255)                                aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                     >  1:3589419/1‑70 (MQ=255)                                aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                     >  1:123839/1‑70 (MQ=255)                                aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                     >  1:2193704/1‑70 (MQ=255)                                aGATCGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                     >  1:4897799/1‑70 (MQ=255)                                 gATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                                   >  1:3407185/1‑71 (MQ=255)                                 gATCGGGTCGCCAGAGAACAGTTCATCGTATTTCGGAGTACGCAGGAAGCGa                                                      >  1:190192/1‑52 (MQ=255)                                 gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGc                                                               <  1:2008814/43‑1 (MQ=255)                                 gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                                     >  1:4145257/1‑53 (MQ=255)                                 gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                                     >  1:5165173/1‑53 (MQ=255)                                 gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                                     >  1:2798119/1‑53 (MQ=255)                                  aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGa                                                                     >  1:440374/1‑36 (MQ=255)                                  aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGa                                                                     >  1:5447180/1‑36 (MQ=255)                                  aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                                  <  1:3607582/71‑1 (MQ=255)                                   ttgggTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                                   >  1:1024587/3‑69 (MQ=255)                                   tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCa                                                              >  1:352507/1‑42 (MQ=255)                                   tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa                                                      >  1:1816350/1‑50 (MQ=255)                                   tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                                >  1:3601350/1‑56 (MQ=255)                                   tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                                >  1:23699/1‑56 (MQ=255)                                   tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                 <  1:3888221/71‑1 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                                   >  1:3362506/1‑52 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                                   >  1:5637492/1‑52 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                                   >  1:2447193/1‑52 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                                   >  1:5177806/1‑52 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                        >  1:722803/1‑63 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                      >  1:5626028/1‑65 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                 <  1:2468755/70‑1 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                 <  1:5527717/70‑1 (MQ=255)                                    cGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCg                                                       >  1:5453611/1‑48 (MQ=255)                                      ggTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                                  <  1:2814762/51‑1 (MQ=255)                                         cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtg                            >  1:4023666/1‑70 (MQ=255)                                          gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                       >  1:3567643/1‑42 (MQ=255)                                          gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                                  >  1:4516847/1‑63 (MQ=255)                                          gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                          >  1:3271539/1‑71 (MQ=255)                                          gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                          >  1:5303577/1‑71 (MQ=255)                                            cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                        <  1:5031927/71‑1 (MQ=255)                                            cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGt                                          <  1:228596/53‑1 (MQ=255)                                            cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                        <  1:278814/71‑1 (MQ=255)                                             agagAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAg                               >  1:1972603/1‑63 (MQ=255)                                              gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                                <  1:4348556/45‑1 (MQ=255)                                              gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                       >  1:878281/1‑69 (MQ=255)                                              gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                       >  1:2931471/1‑69 (MQ=255)                                                gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa                    <  1:1885619/71‑1 (MQ=255)                                                  aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt                  >  1:1719359/1‑71 (MQ=255)                                                  aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt                  >  1:4089161/1‑71 (MQ=255)                                                   cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                  >  1:2999037/1‑70 (MQ=255)                                                      ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                        <  1:4923374/45‑1 (MQ=255)                                                      ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                        <  1:4537443/45‑1 (MQ=255)                                                      ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                        <  1:64744/45‑1 (MQ=255)                                                      ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                        <  1:2220146/45‑1 (MQ=255)                                                       tCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAg                                           >  1:2543439/1‑41 (MQ=255)                                                       tCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAg                                           >  1:4645613/1‑41 (MQ=255)                                                        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                      >  1:5633186/1‑45 (MQ=255)                                                        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                      >  1:2829690/1‑45 (MQ=255)                                                        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac            >  1:3684893/1‑69 (MQ=255)                                                        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc            >  1:3693472/1‑71 (MQ=255)                                                        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc            >  1:1981534/1‑71 (MQ=255)                                                        cATCGTATTCCGGAGTAAGCAGGAAGCGAACCATACGCAGTTTCa                                      >  1:5174622/1‑45 (MQ=255)                                                           caaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgc          >  1:1560493/4‑70 (MQ=255)                                                            gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt        >  1:4107251/1‑71 (MQ=255)                                                            aaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcc         <  1:939224/68‑2 (MQ=255)                                                              aTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                                   >  1:4940931/1‑42 (MQ=255)                                                               ttCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGatg                                 >  1:4958715/1‑41 (MQ=255)                                                               ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTcta                       >  1:958715/1‑51 (MQ=255)                                                               ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGcttctt     >  1:5283728/1‑71 (MQ=255)                                                                tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                  <  1:5318468/57‑1 (MQ=255)                                                                tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                  <  1:4064226/57‑1 (MQ=255)                                                                 ccGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCaa                       >  1:1607554/1‑51 (MQ=255)                                                                  cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt  <  1:4578514/71‑1 (MQ=255)                                                                  cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt  <  1:236672/71‑1 (MQ=255)                                                                    gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                        <  1:3155037/47‑1 (MQ=255)                                                                    gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAttt                 <  1:4530147/54‑1 (MQ=255)                                                                     |                                                                    AGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTT  >  minE/609223‑609358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑