Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,318	T→C	22.9%	K312K (AAA→AAG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,318	0	T	C	22.9%	146.3 / 41.9	83	K312K (AAA→AAG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (40/24);  new base C (9/10);  total (49/34)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGT  >  minE/609250‑609387                                                                     |                                                                      gCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGtt                                                                      >  1:1803539/1‑70 (MQ=255)  cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                                                    <  1:3545860/71‑1 (MQ=255)  cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                                                    <  1:5186223/71‑1 (MQ=255)   ccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                                                   >  1:3512965/1‑71 (MQ=255)    cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                                                                  <  1:3130415/71‑1 (MQ=255)    cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                                                                  <  1:2266684/71‑1 (MQ=255)     aGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                                                                  >  1:3455217/1‑70 (MQ=255)     aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                                                  >  1:4891136/1‑70 (MQ=255)     aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                                                  >  1:123839/1‑70 (MQ=255)     aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                                                  >  1:3794498/1‑70 (MQ=255)     aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                                                  >  1:3589419/1‑70 (MQ=255)     aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                                                  >  1:2193704/1‑70 (MQ=255)     aGATCGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                                                                  >  1:4897799/1‑70 (MQ=255)      gATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                                                                >  1:3407185/1‑71 (MQ=255)       aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                                                               <  1:3607582/71‑1 (MQ=255)        ttgggTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                                                                >  1:1024587/3‑69 (MQ=255)        tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                                              <  1:3888221/71‑1 (MQ=255)         cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                                                     >  1:722803/1‑63 (MQ=255)         cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                                                   >  1:5626028/1‑65 (MQ=255)         cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                                              <  1:5527717/70‑1 (MQ=255)         cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                                              <  1:2468755/70‑1 (MQ=255)              cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtg                                                         >  1:4023666/1‑70 (MQ=255)               gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                                                               >  1:4516847/1‑63 (MQ=255)               gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                                                       >  1:3271539/1‑71 (MQ=255)               gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                                                       >  1:5303577/1‑71 (MQ=255)                 cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                     <  1:5031927/71‑1 (MQ=255)                 cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGt                                                                       <  1:228596/53‑1 (MQ=255)                 cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                     <  1:278814/71‑1 (MQ=255)                  agagAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAg                                                            >  1:1972603/1‑63 (MQ=255)                   gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                                                    >  1:2931471/1‑69 (MQ=255)                   gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                                                    >  1:878281/1‑69 (MQ=255)                     gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa                                                 <  1:1885619/71‑1 (MQ=255)                       aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt                                               >  1:1719359/1‑71 (MQ=255)                       aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt                                               >  1:4089161/1‑71 (MQ=255)                        cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                               >  1:2999037/1‑70 (MQ=255)                           ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                                                     <  1:4923374/45‑1 (MQ=255)                           ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                                                     <  1:4537443/45‑1 (MQ=255)                           ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                                                     <  1:64744/45‑1 (MQ=255)                           ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                                                     <  1:2220146/45‑1 (MQ=255)                             cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                                                   >  1:5633186/1‑45 (MQ=255)                             cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                                                   >  1:2829690/1‑45 (MQ=255)                             cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac                                         >  1:3684893/1‑69 (MQ=255)                             cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                         >  1:1981534/1‑71 (MQ=255)                             cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                         >  1:3693472/1‑71 (MQ=255)                             cATCGTATTCCGGAGTAAGCAGGAAGCGAACCATACGCAGTTTCa                                                                   >  1:5174622/1‑45 (MQ=255)                                caaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgc                                       >  1:1560493/4‑70 (MQ=255)                                 gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt                                     >  1:4107251/1‑71 (MQ=255)                                 aaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcc                                      <  1:939224/68‑2 (MQ=255)                                   aTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                                                                >  1:4940931/1‑42 (MQ=255)                                    ttCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGatg                                                              >  1:4958715/1‑41 (MQ=255)                                    ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTcta                                                    >  1:958715/1‑51 (MQ=255)                                    ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGcttctt                                  >  1:5283728/1‑71 (MQ=255)                                     tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                               <  1:5318468/57‑1 (MQ=255)                                     tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                               <  1:4064226/57‑1 (MQ=255)                                      ccGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCaa                                                    >  1:1607554/1‑51 (MQ=255)                                       cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                               <  1:236672/71‑1 (MQ=255)                                       cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                               <  1:4578514/71‑1 (MQ=255)                                         gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                          >  1:4909761/3‑58 (MQ=255)                                         gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                          >  1:1729511/3‑58 (MQ=255)                                         gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                     <  1:3155037/47‑1 (MQ=255)                                         gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAttt                                              <  1:4530147/54‑1 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:1205687/60‑3 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:4567928/60‑3 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:2205378/60‑3 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:4251922/60‑3 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:339874/60‑3 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:3122764/60‑3 (MQ=255)                                            tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                     <  1:1301603/60‑3 (MQ=255)                                              cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                             >  1:299515/1‑50 (MQ=255)                                              cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                             >  1:1787213/1‑50 (MQ=255)                                                 aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                             >  1:4362599/1‑47 (MQ=255)                                                 aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                             >  1:1682521/1‑47 (MQ=255)                                                 aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                             >  1:2102882/1‑47 (MQ=255)                                                  ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                               <  1:242077/44‑1 (MQ=255)                                                  ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                               <  1:811417/44‑1 (MQ=255)                                                  ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                    >  1:1523225/1‑71 (MQ=255)                                                  ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                    >  1:299446/1‑71 (MQ=255)                                                  ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                    <  1:3169799/71‑1 (MQ=255)                                                   gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCt                      >  1:4128862/1‑68 (MQ=255)                                                   gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                   >  1:5601422/1‑71 (MQ=255)                                                     aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                    >  1:5283690/1‑68 (MQ=255)                                                     aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                   >  1:2070553/1‑69 (MQ=255)                                                        gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATc                       <  1:4481714/62‑1 (MQ=255)                                                        gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt              <  1:1970934/71‑1 (MQ=255)                                                        gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt              >  1:990197/1‑71 (MQ=255)                                                           ccATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                             >  1:3614193/1‑37 (MQ=255)                                                             aTACGCAGTTTCTTGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                               <  1:30087/49‑1 (MQ=255)                                                                cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc      <  1:297722/71‑1 (MQ=255)                                                                cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc      <  1:4365751/71‑1 (MQ=255)                                                                    gTTTCATGACCAGGTGGTCTACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGt  <  1:5111201/71‑1 (MQ=255)                                                                     tttCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCttt            <  1:960013/60‑1 (MQ=255)                                                                     |                                                                      GCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGT  >  minE/609250‑609387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑