Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,326	C→T	15.9%	V310I (GTC→ATC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,326	0	C	T	15.9%	159.9 / 19.7	69	V310I (GTC→ATC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (34/24);  new base T (3/8);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 9.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATG  >  minE/609256‑609393                                                                       |                                                                    aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                                                                     <  1:3607582/71‑1 (MQ=255)  tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                                                    <  1:3888221/71‑1 (MQ=255)   cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                                                    <  1:2468755/70‑1 (MQ=255)   cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                                                                    <  1:5527717/70‑1 (MQ=255)        cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtg                                                               >  1:4023666/1‑70 (MQ=255)         gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                                                                     >  1:4516847/1‑63 (MQ=255)         gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                                                             >  1:3271539/1‑71 (MQ=255)         gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                                                             >  1:5303577/1‑71 (MQ=255)           cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                           <  1:5031927/71‑1 (MQ=255)           cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                           <  1:278814/71‑1 (MQ=255)            agagAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAg                                                                  >  1:1972603/1‑63 (MQ=255)             gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                                                          >  1:2931471/1‑69 (MQ=255)             gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                                                          >  1:878281/1‑69 (MQ=255)               gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa                                                       <  1:1885619/71‑1 (MQ=255)                 aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt                                                     >  1:1719359/1‑71 (MQ=255)                 aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt                                                     >  1:4089161/1‑71 (MQ=255)                  cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     >  1:2999037/1‑70 (MQ=255)                       cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac                                               >  1:3684893/1‑69 (MQ=255)                       cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                               >  1:3693472/1‑71 (MQ=255)                       cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                               >  1:1981534/1‑71 (MQ=255)                          caaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgc                                             >  1:1560493/4‑70 (MQ=255)                           gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt                                           >  1:4107251/1‑71 (MQ=255)                           aaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcc                                            <  1:939224/68‑2 (MQ=255)                              ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTcta                                                          >  1:958715/1‑51 (MQ=255)                              ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGcttctt                                        >  1:5283728/1‑71 (MQ=255)                               tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:4064226/57‑1 (MQ=255)                               tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:5318468/57‑1 (MQ=255)                                ccGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCaa                                                          >  1:1607554/1‑51 (MQ=255)                                 cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                     <  1:236672/71‑1 (MQ=255)                                 cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                     <  1:4578514/71‑1 (MQ=255)                                   gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                                >  1:4909761/3‑58 (MQ=255)                                   gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                                >  1:1729511/3‑58 (MQ=255)                                   gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                           <  1:3155037/47‑1 (MQ=255)                                   gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAttt                                                    <  1:4530147/54‑1 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:4251922/60‑3 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:4567928/60‑3 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:1205687/60‑3 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:339874/60‑3 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:1301603/60‑3 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:3122764/60‑3 (MQ=255)                                      tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:2205378/60‑3 (MQ=255)                                        cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:299515/1‑50 (MQ=255)                                        cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:1787213/1‑50 (MQ=255)                                           aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:2102882/1‑47 (MQ=255)                                           aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:1682521/1‑47 (MQ=255)                                           aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:4362599/1‑47 (MQ=255)                                            ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:811417/44‑1 (MQ=255)                                            ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:242077/44‑1 (MQ=255)                                            ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          <  1:3169799/71‑1 (MQ=255)                                            ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          >  1:1523225/1‑71 (MQ=255)                                            ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          >  1:299446/1‑71 (MQ=255)                                             gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCt                            >  1:4128862/1‑68 (MQ=255)                                             gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                         >  1:5601422/1‑71 (MQ=255)                                               aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          >  1:5283690/1‑68 (MQ=255)                                               aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                         >  1:2070553/1‑69 (MQ=255)                                                  gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATc                             <  1:4481714/62‑1 (MQ=255)                                                  gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt                    <  1:1970934/71‑1 (MQ=255)                                                  gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt                    >  1:990197/1‑71 (MQ=255)                                                     ccATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:3614193/1‑37 (MQ=255)                                                       aTACGCAGTTTCTTGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                     <  1:30087/49‑1 (MQ=255)                                                          cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc            <  1:4365751/71‑1 (MQ=255)                                                          cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc            <  1:297722/71‑1 (MQ=255)                                                              gTTTCATGACCAGGTGGTCTACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGt        <  1:5111201/71‑1 (MQ=255)                                                               tttCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCttt                  <  1:960013/60‑1 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt                           >  1:422164/1‑49 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt                           >  1:4065374/1‑49 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:2058404/70‑1 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:4254341/70‑1 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:1665705/70‑1 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      >  1:728656/1‑70 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      >  1:3027650/1‑70 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:333195/70‑1 (MQ=255)                                                                 tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:1026504/70‑1 (MQ=255)                                                                    tGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATg  >  1:178792/1‑71 (MQ=255)                                                                       |                                                                    ATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATG  >  minE/609256‑609393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑