Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,329	G→A	16.7%	L309L (CTG→TTG) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,329	0	G	A	16.7%	148.2 / 19.8	68	L309L (CTG→TTG) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (31/24);  new base A (3/8);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

CGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATG  >  minE/609263‑609393                                                                   |                                                                 cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtg                                                               >  1:4023666/1‑70 (MQ=255)  gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                                                             >  1:5303577/1‑71 (MQ=255)  gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                                                             >  1:3271539/1‑71 (MQ=255)    cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                           <  1:5031927/71‑1 (MQ=255)    cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                           <  1:278814/71‑1 (MQ=255)     agagAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAg                                                                  >  1:1972603/1‑63 (MQ=255)      gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                                                          >  1:2931471/1‑69 (MQ=255)      gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                                                          >  1:878281/1‑69 (MQ=255)        gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa                                                       <  1:1885619/71‑1 (MQ=255)          aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt                                                     >  1:1719359/1‑71 (MQ=255)          aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt                                                     >  1:4089161/1‑71 (MQ=255)           cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     >  1:2999037/1‑70 (MQ=255)                cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac                                               >  1:3684893/1‑69 (MQ=255)                cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                               >  1:3693472/1‑71 (MQ=255)                cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                               >  1:1981534/1‑71 (MQ=255)                   caaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgc                                             >  1:1560493/4‑70 (MQ=255)                    gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt                                           >  1:4107251/1‑71 (MQ=255)                    aaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcc                                            <  1:939224/68‑2 (MQ=255)                       ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTcta                                                          >  1:958715/1‑51 (MQ=255)                       ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGcttctt                                        >  1:5283728/1‑71 (MQ=255)                        tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:5318468/57‑1 (MQ=255)                        tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:4064226/57‑1 (MQ=255)                         ccGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCaa                                                          >  1:1607554/1‑51 (MQ=255)                          cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                     <  1:4578514/71‑1 (MQ=255)                          cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                     <  1:236672/71‑1 (MQ=255)                            gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                                >  1:1729511/3‑58 (MQ=255)                            gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                                >  1:4909761/3‑58 (MQ=255)                            gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                                                           <  1:3155037/47‑1 (MQ=255)                            gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAttt                                                    <  1:4530147/54‑1 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:4251922/60‑3 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:1205687/60‑3 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:4567928/60‑3 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:3122764/60‑3 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:1301603/60‑3 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:2205378/60‑3 (MQ=255)                               tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                           <  1:339874/60‑3 (MQ=255)                                 cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:299515/1‑50 (MQ=255)                                 cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:1787213/1‑50 (MQ=255)                                    aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:2102882/1‑47 (MQ=255)                                    aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:4362599/1‑47 (MQ=255)                                    aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:1682521/1‑47 (MQ=255)                                     ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:811417/44‑1 (MQ=255)                                     ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                     <  1:242077/44‑1 (MQ=255)                                     ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          >  1:1523225/1‑71 (MQ=255)                                     ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          <  1:3169799/71‑1 (MQ=255)                                     ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          >  1:299446/1‑71 (MQ=255)                                      gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCt                            >  1:4128862/1‑68 (MQ=255)                                      gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                         >  1:5601422/1‑71 (MQ=255)                                        aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                          >  1:5283690/1‑68 (MQ=255)                                        aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                         >  1:2070553/1‑69 (MQ=255)                                           gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATc                             <  1:4481714/62‑1 (MQ=255)                                           gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt                    >  1:990197/1‑71 (MQ=255)                                           gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt                    <  1:1970934/71‑1 (MQ=255)                                              ccATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                   >  1:3614193/1‑37 (MQ=255)                                                aTACGCAGTTTCTTGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                     <  1:30087/49‑1 (MQ=255)                                                   cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc            <  1:4365751/71‑1 (MQ=255)                                                   cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc            <  1:297722/71‑1 (MQ=255)                                                       gTTTCATGACCAGGTGGTCTACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGt        <  1:5111201/71‑1 (MQ=255)                                                        tttCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCttt                  <  1:960013/60‑1 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt                           >  1:422164/1‑49 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt                           >  1:4065374/1‑49 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:4254341/70‑1 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:1026504/70‑1 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:1665705/70‑1 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      >  1:728656/1‑70 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:2058404/70‑1 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      >  1:3027650/1‑70 (MQ=255)                                                          tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc      <  1:333195/70‑1 (MQ=255)                                                             tGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATg  >  1:178792/1‑71 (MQ=255)                                                                 cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCa                >  1:2169634/1‑53 (MQ=255)                                                                 cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAg               >  1:2332258/1‑54 (MQ=255)                                                                 cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAg               >  1:1121064/1‑54 (MQ=255)                                                                   ggtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCa                >  1:680557/1‑51 (MQ=255)                                                                   |                                                                 CGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATG  >  minE/609263‑609393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑