| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,333 | G→A | 16.2% | D307D (GAC→GAT) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,333 | 0 | G | A | 16.2% | 155.7 / 19.6 | 68 | D307D (GAC→GAT) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (33/24); new base A (3/8); total (36/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.83e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
GCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCA > minE/609264‑609403 | gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt > 1:3271539/1‑71 (MQ=255)gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt > 1:5303577/1‑71 (MQ=255) cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa < 1:5031927/71‑1 (MQ=255) cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa < 1:278814/71‑1 (MQ=255) gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat > 1:2931471/1‑69 (MQ=255) gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat > 1:878281/1‑69 (MQ=255) gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa < 1:1885619/71‑1 (MQ=255) aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt > 1:1719359/1‑71 (MQ=255) aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt > 1:4089161/1‑71 (MQ=255) cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt > 1:2999037/1‑70 (MQ=255) cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac > 1:3684893/1‑69 (MQ=255) cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc > 1:1981534/1‑71 (MQ=255) cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc > 1:3693472/1‑71 (MQ=255) caaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgc > 1:1560493/4‑70 (MQ=255) gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt > 1:4107251/1‑71 (MQ=255) aaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcc < 1:939224/68‑2 (MQ=255) ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTcta > 1:958715/1‑51 (MQ=255) ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGcttctt > 1:5283728/1‑71 (MQ=255) tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt < 1:5318468/57‑1 (MQ=255) tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt < 1:4064226/57‑1 (MQ=255) ccGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCaa > 1:1607554/1‑51 (MQ=255) cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt < 1:236672/71‑1 (MQ=255) cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt < 1:4578514/71‑1 (MQ=255) gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg > 1:1729511/3‑58 (MQ=255) gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg > 1:4909761/3‑58 (MQ=255) gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa < 1:3155037/47‑1 (MQ=255) gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAttt < 1:4530147/54‑1 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:339874/60‑3 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:3122764/60‑3 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:4567928/60‑3 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:4251922/60‑3 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:1205687/60‑3 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:2205378/60‑3 (MQ=255) tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct < 1:1301603/60‑3 (MQ=255) cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc > 1:299515/1‑50 (MQ=255) cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc > 1:1787213/1‑50 (MQ=255) aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc > 1:2102882/1‑47 (MQ=255) aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc > 1:4362599/1‑47 (MQ=255) aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc > 1:1682521/1‑47 (MQ=255) ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt < 1:242077/44‑1 (MQ=255) ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt < 1:811417/44‑1 (MQ=255) ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg > 1:299446/1‑71 (MQ=255) ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg > 1:1523225/1‑71 (MQ=255) ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg < 1:3169799/71‑1 (MQ=255) gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCt > 1:4128862/1‑68 (MQ=255) gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc > 1:5601422/1‑71 (MQ=255) aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg > 1:5283690/1‑68 (MQ=255) aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc > 1:2070553/1‑69 (MQ=255) gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATc < 1:4481714/62‑1 (MQ=255) gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt > 1:990197/1‑71 (MQ=255) gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt < 1:1970934/71‑1 (MQ=255) ccATACGCAGTTTCATGACCAGGTGGTCAACCATTTc > 1:3614193/1‑37 (MQ=255) aTACGCAGTTTCTTGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt < 1:30087/49‑1 (MQ=255) cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc < 1:4365751/71‑1 (MQ=255) cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc < 1:297722/71‑1 (MQ=255) gTTTCATGACCAGGTGGTCTACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGt < 1:5111201/71‑1 (MQ=255) tttCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCttt < 1:960013/60‑1 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt > 1:422164/1‑49 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt > 1:4065374/1‑49 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc < 1:4254341/70‑1 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc < 1:1026504/70‑1 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc < 1:1665705/70‑1 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc > 1:728656/1‑70 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc < 1:2058404/70‑1 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc > 1:3027650/1‑70 (MQ=255) tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc < 1:333195/70‑1 (MQ=255) tGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATg > 1:178792/1‑71 (MQ=255) cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCa > 1:2169634/1‑53 (MQ=255) cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAg > 1:2332258/1‑54 (MQ=255) cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAg > 1:1121064/1‑54 (MQ=255) ggtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCa > 1:680557/1‑51 (MQ=255) gtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACAt > 1:5601343/1‑71 (MQ=255) ggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCa > 1:2663644/1‑41 (MQ=255) gtCAACCATTTCCTGCTCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATcc < 1:1769834/70‑1 (MQ=255) gtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATc > 1:2417452/1‑69 (MQ=255) gtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATc > 1:4725418/1‑69 (MQ=255) gtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCa < 1:4826755/71‑1 (MQ=255) | GCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCA > minE/609264‑609403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |