Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 609,338 C→T 14.8% V306I (GTT→ATT) ‡ pflB ← pyruvate formate lyase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE609,3380CT14.8% 174.6 / 19.3 74V306I (GTT→ATT) ‡pflBpyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base C (35/28);  new base T (3/8);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

GAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGA  >  minE/609270‑609406
                                                                    |                                                                    
gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa                                                                    <  1:1885619/71‑1 (MQ=255)
  aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt                                                                  >  1:1719359/1‑71 (MQ=255)
  aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt                                                                  >  1:4089161/1‑71 (MQ=255)
   cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                                  >  1:2999037/1‑70 (MQ=255)
        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac                                                            >  1:3684893/1‑69 (MQ=255)
        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                                            >  1:1981534/1‑71 (MQ=255)
        cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc                                                            >  1:3693472/1‑71 (MQ=255)
           caaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgc                                                          >  1:1560493/4‑70 (MQ=255)
            gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt                                                        >  1:4107251/1‑71 (MQ=255)
            aaaTTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcc                                                         <  1:939224/68‑2 (MQ=255)
               ttCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGcttctt                                                     >  1:5283728/1‑71 (MQ=255)
                tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                                  <  1:4064226/57‑1 (MQ=255)
                tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                                  <  1:5318468/57‑1 (MQ=255)
                  cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                                  <  1:4578514/71‑1 (MQ=255)
                  cGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                                  <  1:236672/71‑1 (MQ=255)
                    gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                                             >  1:4909761/3‑58 (MQ=255)
                    gtgTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTg                                                             >  1:1729511/3‑58 (MQ=255)
                    gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAttt                                                                 <  1:4530147/54‑1 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:4251922/60‑3 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:1301603/60‑3 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:339874/60‑3 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:4567928/60‑3 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:1205687/60‑3 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:2205378/60‑3 (MQ=255)
                       tACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTgtgcct                                                        <  1:3122764/60‑3 (MQ=255)
                         cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                                >  1:299515/1‑50 (MQ=255)
                         cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                                >  1:1787213/1‑50 (MQ=255)
                            aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                                >  1:2102882/1‑47 (MQ=255)
                            aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                                >  1:1682521/1‑47 (MQ=255)
                            aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                                >  1:4362599/1‑47 (MQ=255)
                             ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                                  <  1:242077/44‑1 (MQ=255)
                             ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt                                                                  <  1:811417/44‑1 (MQ=255)
                             ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                                       >  1:299446/1‑71 (MQ=255)
                             ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                                       >  1:1523225/1‑71 (MQ=255)
                             ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                                       <  1:3169799/71‑1 (MQ=255)
                              gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCt                                         >  1:4128862/1‑68 (MQ=255)
                              gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                                      >  1:5601422/1‑71 (MQ=255)
                                aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTg                                       >  1:5283690/1‑68 (MQ=255)
                                aGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGc                                      >  1:2070553/1‑69 (MQ=255)
                                   gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATc                                          <  1:4481714/62‑1 (MQ=255)
                                   gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt                                 >  1:990197/1‑71 (MQ=255)
                                   gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCt                                 <  1:1970934/71‑1 (MQ=255)
                                      ccATACGCAGTTTCATGACCAGGTGGTCAACCATTTc                                                                >  1:3614193/1‑37 (MQ=255)
                                        aTACGCAGTTTCTTGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGtt                                                  <  1:30087/49‑1 (MQ=255)
                                           cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc                         <  1:4365751/71‑1 (MQ=255)
                                           cGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTc                         <  1:297722/71‑1 (MQ=255)
                                               gTTTCATGACCAGGTGGTCTACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGt                     <  1:5111201/71‑1 (MQ=255)
                                                tttCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCttt                               <  1:960013/60‑1 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt                                        >  1:4065374/1‑49 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCtt                                        >  1:422164/1‑49 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   >  1:728656/1‑70 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   <  1:333195/70‑1 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   <  1:4254341/70‑1 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   <  1:1026504/70‑1 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   >  1:3027650/1‑70 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   <  1:1665705/70‑1 (MQ=255)
                                                  tCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTc                   <  1:2058404/70‑1 (MQ=255)
                                                     tGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATg               >  1:178792/1‑71 (MQ=255)
                                                         cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCa                             >  1:2169634/1‑53 (MQ=255)
                                                         cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAg                            >  1:1121064/1‑54 (MQ=255)
                                                         cAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAg                            >  1:2332258/1‑54 (MQ=255)
                                                           ggtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCa                             >  1:680557/1‑51 (MQ=255)
                                                            gtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACAt        >  1:5601343/1‑71 (MQ=255)
                                                              ggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCa                                    >  1:2663644/1‑41 (MQ=255)
                                                               gtCAACCATTTCCTGCTCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATcc      <  1:1769834/70‑1 (MQ=255)
                                                               gtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATc       >  1:2417452/1‑69 (MQ=255)
                                                               gtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATc       >  1:4725418/1‑69 (MQ=255)
                                                               gtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCa     <  1:4826755/71‑1 (MQ=255)
                                                                 cAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTa             <  1:5674027/61‑1 (MQ=255)
                                                                  aaCCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAgg   >  1:4826953/1‑70 (MQ=255)
                                                                  aaCCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAgg   <  1:3425056/70‑1 (MQ=255)
                                                                  aaCCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGa  <  1:1648544/71‑1 (MQ=255)
                                                                  aaCCATTTACTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGa  <  1:2019610/71‑1 (MQ=255)
                                                                   aCCATTTCCTGCGCTTCTTGTTCGGTGATCTTGGCAGCTTTCAGGTCACGTTc                   <  1:275840/53‑1 (MQ=255)
                                                                    |                                                                    
GAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGA  >  minE/609270‑609406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: