BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	609,720	G→A	20.8%	I178I (ATC→ATT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	609,720	0	G	A	20.8%	220.5 / 46.7	106	I178I (ATC→ATT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (51/32); new base A (9/13); total (60/46)
Fisher's exact test for biased strand distribution p-value = 9.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 125 total aligned reads displayed.

AACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTC  >  minE/609652‑609788

 aCTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                                     <  1:4533258/69‑1 (MQ=255)

  cTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                                     <  1:1501583/68‑1 (MQ=255)

   tgtgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacg                                                                 >  1:227242/1‑71 (MQ=255)

     tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                                     >  1:549199/1‑65 (MQ=255)

     tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga                                                                     >  1:5575671/1‑65 (MQ=255)

     tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggc                                                               >  1:58150/1‑71 (MQ=255)

        cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac                                                            <  1:3335765/71‑1 (MQ=255)

        cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac                                                            >  1:4086692/1‑71 (MQ=255)

             tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCGCCgatgat                                                                    >  1:1554669/1‑58 (MQ=255)

             tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatgat                                                                    >  1:5210927/1‑58 (MQ=255)

             tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac                                                            >  1:3453361/1‑66 (MQ=255)

              gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       >  1:4368058/1‑70 (MQ=255)

              gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:4987662/70‑1 (MQ=255)

              gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:4981263/70‑1 (MQ=255)

                cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                                      >  1:1497996/1‑53 (MQ=255)

                cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                                      >  1:1698368/1‑53 (MQ=255)

                cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                                      >  1:1216460/1‑53 (MQ=255)

                cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg                                                                      >  1:4441436/1‑53 (MQ=255)

                  ttCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata                                                     >  1:572454/1‑68 (MQ=255)

                   tcatcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGc                                                  <  1:2795615/70‑1 (MQ=255)

                     atcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                                <  1:739018/70‑1 (MQ=255)

                      tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt                                              <  1:2921582/71‑1 (MQ=255)

                      tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt                                              <  1:5165410/71‑1 (MQ=255)

                          gTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:3335217/58‑1 (MQ=255)

                             gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc                                       <  1:1626945/71‑1 (MQ=255)

                               cGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCg                                     >  1:3647090/1‑71 (MQ=255)

                                gATACCTTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                    >  1:2364034/1‑71 (MQ=255)

                                gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:2112933/52‑1 (MQ=255)

                                gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg                                    >  1:4304771/1‑71 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccacggc                                                         >  1:3762709/1‑48 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                                >  1:5246443/1‑57 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                                >  1:867204/1‑57 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                                >  1:2625745/1‑57 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt                                                >  1:5135949/1‑57 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt                                   >  1:652340/1‑70 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt                                   >  1:1882342/1‑70 (MQ=255)

                                  tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGACACGGCCATATGCAt                                                >  1:5125436/1‑57 (MQ=255)

                                    ccatACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATGTGGTAAACCGGTCAg                                <  1:3584754/68‑1 (MQ=255)

                                    ccatACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAg                                <  1:1652425/68‑1 (MQ=255)

                                    ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata                                                     <  1:3301076/50‑1 (MQ=255)

                                    ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata                                                     <  1:1825870/50‑1 (MQ=255)

                                    ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg                                <  1:4061678/71‑1 (MQ=255)

                                    ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg                                <  1:4882749/71‑1 (MQ=255)

                                       tACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa                                                 <  1:4261029/51‑1 (MQ=255)

                                        aCAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCacg                            >  1:2452247/1‑70 (MQ=255)

                                        aCAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa                                                 <  1:3353916/50‑1 (MQ=255)

                                         cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                                           >  1:2969872/1‑54 (MQ=16)

                                         cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                                           >  1:2963213/1‑54 (MQ=16)

                                         cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                                           >  1:1897407/1‑54 (MQ=16)

                                         cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt                                           >  1:2959397/1‑54 (MQ=16)

                                         cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc                                               >  1:3114443/1‑51 (MQ=255)

                                         cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc                                               >  1:2302745/1‑51 (MQ=255)

                                         cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                           >  1:2925148/1‑71 (MQ=255)

                                         cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                           >  1:4111320/1‑71 (MQ=255)

                                         cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                           <  1:2323848/71‑1 (MQ=255)

                                           gcgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                                          <  1:111725/54‑3 (MQ=12)

                                            cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                                          <  1:1037542/53‑3 (MQ=12)

                                            cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                                          <  1:4648563/53‑3 (MQ=12)

                                            cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta                                          <  1:1468219/53‑3 (MQ=12)

                                            cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                           >  1:5112888/1‑68 (MQ=255)

                                            cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                           >  1:1550717/1‑68 (MQ=255)

                                             gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGa                       <  1:3725503/68‑1 (MQ=255)

                                             gcAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:4708354/39‑1 (MQ=255)

                                             gcAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:4342510/39‑1 (MQ=255)

                                             gcAACGCGACGGTAGTCACCGATGATAcggccacggcca                                                       <  1:2937721/39‑1 (MQ=255)

                                               aaCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                     <  1:2322149/71‑1 (MQ=255)

                                               aaCGCGACGGTAGTCACAGATGATACGGCCAAGGCCATATGCATCTGGCAGAc                                       >  1:1878734/1‑53 (MQ=255)

                                                aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                     >  1:343396/1‑70 (MQ=255)

                                                 cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACGGGTCAGCACGCCAGAtt                     <  1:2976377/69‑1 (MQ=255)

                                                 cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTa                   >  1:1043154/1‑71 (MQ=255)

                                                 cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                   >  1:1977826/1‑71 (MQ=255)

                                                 cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                   <  1:4904901/71‑1 (MQ=255)

                                                 cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                   <  1:3262171/71‑1 (MQ=255)

                                                  gcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGCTCAGAACACCAGATTTAc                  <  1:2408572/71‑1 (MQ=255)

                                                     aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc               <  1:1586519/71‑1 (MQ=255)

                                                     aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc               <  1:1593060/71‑1 (MQ=255)

                                                         tAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACg           >  1:3230450/1‑71 (MQ=255)

                                                          aGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAac                             >  1:4555955/1‑52 (MQ=255)

                                                          aGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAac                             >  1:871138/1‑52 (MQ=255)

                                                          aGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAac                             >  1:2650020/1‑52 (MQ=255)

                                                          aGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAac                             >  1:4743364/1‑52 (MQ=255)

                                                           gTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACg           >  1:1429881/1‑69 (MQ=255)

                                                           gTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGAttt                    <  1:267034/60‑1 (MQ=255)

                                                            tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCa                         <  1:3137773/54‑1 (MQ=255)

                                                            tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCa                         <  1:5566384/54‑1 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                 >  1:4480444/1‑60 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                 >  1:5302453/1‑60 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:5385352/1‑71 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:5506485/1‑71 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:2137121/1‑71 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:1634714/1‑71 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:3452840/1‑71 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:3952179/1‑71 (MQ=255)

                                                              aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa      >  1:830147/1‑71 (MQ=255)

                                                                cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg    <  1:269486/71‑1 (MQ=255)

                                                                 gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                   >  1:4793356/1‑55 (MQ=255)

                                                                 gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                   >  1:2434345/1‑55 (MQ=255)

                                                                 gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                 >  1:2412064/1‑57 (MQ=255)

                                                                 gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                 >  1:3314651/1‑57 (MQ=255)

                                                                 gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat   >  1:840490/1‑69 (MQ=255)

AACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTC  >  minE/609652‑609788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`