| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 18,192 | C→A | 10.4% | intergenic (+31/+301) | nhaR → / ← rpsT | DNA‑binding transcriptional activator/30S ribosomal subunit protein S20 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 18,192 | 0 | C | A | 10.4% | 208.0 / 7.3 | 67 | intergenic (+31/+301) | nhaR/rpsT | DNA‑binding transcriptional activator/30S ribosomal subunit protein S20 |
| Reads supporting (aligned to +/- strand): ref base C (24/36); new base A (4/3); total (28/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
TTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACA > minE/18131‑18260 | ttCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCaaa < 1:4199485/70‑1 (MQ=255) tttAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACcatc < 1:3612578/69‑1 (MQ=255) gCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAAt < 1:994125/71‑1 (MQ=255) gCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAAt < 1:4388896/71‑1 (MQ=255) tGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACcatc < 1:1031055/56‑1 (MQ=255) ttAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGt < 1:909484/71‑1 (MQ=255) aaTCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTg > 1:5665668/1‑71 (MQ=255) aTCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTgg < 1:4354530/71‑1 (MQ=255) aTCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTgg < 1:4007014/71‑1 (MQ=255) tCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTaa < 1:1640604/57‑1 (MQ=255) cTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAACCACCATCATACACTAAATCAGTAAGTTGGCAGCATCa > 1:1008144/1‑71 (MQ=255) cccccAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATa > 1:2704915/1‑42 (MQ=255) ccccAAAGTTAAGGTTGGAGCACCTCAAAAACACcatca < 1:4319724/39‑1 (MQ=255) ccAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCacctac > 1:255250/1‑71 (MQ=255) aaGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTc < 1:4907359/71‑1 (MQ=255) aGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTc > 1:1682707/1‑70 (MQ=255) actggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCaaaaaagcgactca < 1:5137758‑M1/69‑15 (MQ=255) actggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCaaaaaagcgactca > 1:2263577‑M1/3‑57 (MQ=255) actGGAGCACCTCAAAAACACCATCATACACTAAATCAGt < 1:561254/38‑1 (MQ=255) actGGAGCACCTCAAAAACACCATCATACACTAAATCAGt < 1:2849870/38‑1 (MQ=255) actGGAGCACCTCAAAAACACCATCATACACTAAATCAGt < 1:5634696/38‑1 (MQ=255) actGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgca < 1:1946613/47‑1 (MQ=255) actGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgca < 1:5346513/47‑1 (MQ=255) actGGAGAACCTCAAAAACACCATCATACACTAAATCAGTAAGtt < 1:3315589/43‑1 (MQ=255) ctGGAGCACCTCAAAAACACCATCATACACTAAATCAg < 1:1532448/37‑1 (MQ=255) ctGGAGCACCTCAAAAACACCATCATACACTAAATCAg < 1:4652186/37‑1 (MQ=255) ctGGAGCACCTCAAAAACACCATCATACACTAAATCAg < 1:395446/37‑1 (MQ=255) ctGGAGCACCTCAAAAACACCATCATACACTAAATCAGTaa < 1:2163445/40‑1 (MQ=255) ctGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGt < 1:942139/42‑1 (MQ=255) ctGGAGAACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCAt < 1:2326418/51‑1 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTaa > 1:2958711/1‑40 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCAt < 1:3124528/51‑1 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCAt < 1:5616841/51‑1 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCaccttt < 1:2594360/58‑3 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCac > 1:4495373/1‑54 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCac > 1:3415163/1‑54 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCaaaa < 1:2905206‑M1/59‑5 (MQ=255) tGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCaaaa < 1:2093994‑M1/59‑5 (MQ=255) tGGAGAACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgc < 1:4214973/46‑1 (MQ=255) tGGAGAACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCcgtattgtccatgcga > 1:5010402‑M1/1‑55 (MQ=255) tGGAGAACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACAcgtattgtccatgcga > 1:5244862‑M1/1‑55 (MQ=255) ggAGCACCTCAAAAACACCATCATACTCTAAATCAGTAAGTTGGCAGCATCACCaaaaaagcgactcacat > 1:4556855‑M1/1‑54 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCa < 1:528705/35‑1 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTaa > 1:4744654/1‑39 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGt > 1:3385409/1‑41 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTg > 1:1922725/1‑43 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTg > 1:2543590/1‑43 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgcag < 1:3822309/47‑1 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCAt > 1:1370068/1‑50 (MQ=255) ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCAt > 1:5087899/1‑50 (MQ=255) ggAGAACCTCAAAAACACCATCATACACTAAATCAGTa > 1:4631326/1‑38 (MQ=255) ggAGAACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCa > 1:1743335/1‑52 (MQ=255) gagaACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgcagca > 1:4527828/1‑48 (MQ=255) gagaACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgcagca > 1:3083399/1‑48 (MQ=255) gagaACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCcgtattgtccatgcgatg < 1:2711398‑M1/71‑19 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAACTCAGTAAGTTGGCAGCATCCCCaaaaaagcgactcaca < 1:4669689‑M1/69‑17 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAg > 1:5228150/1‑39 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAg > 1:5334881/1‑39 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAg > 1:5315803/1‑39 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGt > 1:285678/1‑40 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTgg > 1:1502344/1‑43 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTgg > 1:1593612/1‑43 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGgca > 1:5284182/1‑45 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCaaaaaagcgactcaca < 1:5532234‑M1/69‑17 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATcac < 1:76182/71‑1 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATc < 1:5336068/69‑1 (MQ=255) gAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATc < 1:4497109/69‑1 (MQ=255) gAGCACCTCAAAAACACAATCATACACTAAATCAGTaa > 1:4313716/1‑38 (MQ=255) aGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTgg < 1:541411/42‑1 (MQ=255) aGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATcaca < 1:3657564/71‑1 (MQ=255) cACCTCAAAAACACCATCATACACTAAATCAGTAAGtt > 1:2973373/1‑38 (MQ=255) cACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATc > 1:5546148/1‑47 (MQ=255) | TTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCCAAAGTTAAGGTTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTACCTCAATGTGTATCACA > minE/18131‑18260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |