| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 609,783 | G→C | 29.8% | I157M (ATC→ATG) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 609,783 | 0 | G | C | 29.8% | 167.9 / 87.5 | 114 | I157M (ATC→ATG) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (55/25); new base C (17/17); total (72/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.86e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
ACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTG > minE/609714‑609852 | aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa > 1:1634714/1‑71 (MQ=255)aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa > 1:830147/1‑71 (MQ=255)aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa > 1:1910296/1‑71 (MQ=255)aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa > 1:5506485/1‑71 (MQ=255)aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa > 1:2137121/1‑71 (MQ=255)aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa > 1:3452840/1‑71 (MQ=255)aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACAGGTCAGAACACCAGATTTACGGCAACGCAgg < 1:438047/70‑1 (MQ=255) cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg < 1:269486/71‑1 (MQ=255) gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat > 1:840490/1‑69 (MQ=255) atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc > 1:417548/1‑71 (MQ=255) atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc > 1:3351202/1‑71 (MQ=255) atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg < 1:1230781/70‑1 (MQ=255) atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg > 1:5603563/1‑70 (MQ=255) cgcccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAt > 1:291098/4‑62 (MQ=255) cgcccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAt > 1:2696991/4‑62 (MQ=255) ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGTCAACGCAGGATGTCCGGAGTGTa < 1:4426013/71‑1 (MQ=255) ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:119365/71‑1 (MQ=255) cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaa < 1:609488/71‑1 (MQ=255) cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:472761/70‑1 (MQ=255) cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa < 1:5234997/71‑1 (MQ=255) acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:826443/69‑1 (MQ=255) acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:5222851/69‑1 (MQ=255) cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:1699264/1‑70 (MQ=255) cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc > 1:5312540/1‑71 (MQ=255) cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc > 1:2896784/1‑71 (MQ=255) ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACg < 1:114108/71‑1 (MQ=255) ggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAca < 1:5066423/71‑2 (MQ=255) ccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATc < 1:4805222/71‑1 (MQ=255) caTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg < 1:1392433/71‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGAt < 1:1389149/50‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:2781513/62‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa < 1:1112119/71‑1 (MQ=255) tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa < 1:4442888/71‑1 (MQ=255) agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa > 1:2467907/4‑70 (MQ=255) tGCATCTGGCAGACCGGGCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3822322/1‑71 (MQ=255) gccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa > 1:35780/3‑62 (MQ=255) gccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaat < 1:4228793/68‑2 (MQ=255) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc < 1:2465098/48‑1 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc < 1:4288415/48‑1 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggtg < 1:3678607/52‑3 (MQ=16) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:685436/56‑1 (MQ=255) tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa < 1:145809/56‑1 (MQ=255) cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc > 1:4247352/1‑71 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:1846544/54‑1 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCt > 1:5075076/1‑71 (MQ=255) tGGCAGACCGGTCAGAACACCAGATTTACGG‑AACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg > 1:2475998/1‑71 (MQ=255) cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:3868989/1‑60 (MQ=255) cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:4702682/1‑60 (MQ=255) aaaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGt > 1:1024750/3‑70 (MQ=255) gACCGGTCAGAACACCAGATTTACGGCAACGCAgg < 1:1134571/35‑1 (MQ=255) gACCGGTCAGAACACCAGATTTACGGCAACGCAgg > 1:1265131/1‑35 (MQ=255) ccGGTCAGAACACCAGATTTACGGCAACGCAGGAt > 1:2787592/1‑35 (MQ=255) cGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTtatgg > 1:3710304/1‑70 (MQ=255) ggTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa < 1:3069347/45‑1 (MQ=255) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4575174/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4581481/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:4106300/1‑60 (MQ=12) gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc > 1:5571530/1‑60 (MQ=12) gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:1149188/1‑55 (MQ=255) gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAac > 1:1333273/1‑54 (MQ=255) tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:2025101/1‑45 (MQ=255) tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa > 1:3094220/1‑45 (MQ=255) cAGAACACCAGATTTACGGCAACGCATGATGTCCGGAGTGTAAACGTCg < 1:417847/49‑1 (MQ=255) aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt > 1:4540961/1‑70 (MQ=255) gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTTAACGTCGaa > 1:4909086/1‑49 (MQ=255) gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:2810451/1‑49 (MQ=255) gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:1354427/1‑49 (MQ=255) aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3661589/1‑50 (MQ=255) aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:1366394/1‑70 (MQ=255) ccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGtt < 1:1475341/63‑1 (MQ=16) ccAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACgcag < 1:5526446/71‑4 (MQ=255) cAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:4448415/1‑67 (MQ=255) aGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:5020873/1‑66 (MQ=255) aGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt > 1:4220959/1‑71 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:3553148/1‑43 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca > 1:425310/1‑43 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTAtt > 1:5335648/1‑70 (MQ=255) gATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTc < 1:2987674/71‑1 (MQ=255) aTTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGt < 1:1816085/58‑1 (MQ=12) tttAAGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa > 1:5282951/1‑39 (MQ=255) tACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTTATGGGTTTTACg > 1:624521/1‑61 (MQ=16) tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:718323/53‑1 (MQ=255) tACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:5670167/53‑1 (MQ=255) aCGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg > 1:4481659/1‑70 (MQ=255) gCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGa < 1:257130/49‑1 (MQ=255) cAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGtttt > 1:1238475/1‑53 (MQ=255) aaCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtt > 1:3581036/1‑71 (MQ=255) aCGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTg > 1:2324190/1‑64 (MQ=255) cGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttt < 1:1350574/71‑1 (MQ=255) gCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa > 1:1039979/1‑50 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:1739280/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:2460056/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:683568/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACg > 1:2670501/1‑51 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAtttttt > 1:1047864/1‑71 (MQ=255) cAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGAttttt < 1:5433830/70‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAg < 1:3102581/42‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCGGTGAAGATTTTTTTg < 1:1513943/71‑1 (MQ=255) ggATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTg < 1:3189165/71‑1 (MQ=255) gATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa < 1:3490918/46‑1 (MQ=255) | ACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTACGGTATTCAGTGAAGATTTTTTTG > minE/609714‑609852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |