| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 702,791 | G→A | 11.8% | D618D (GAC→GAT) | ymcA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 702,791 | 0 | G | A | 11.8% | 198.8 / 13.8 | 76 | D618D (GAC→GAT) | ymcA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (34/33); new base A (3/6); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
CGCCTTCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCA > minE/702724‑702861 | cgccTTCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCa < 1:3434589/71‑1 (MQ=255)cgccTTCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCa < 1:1599718/71‑1 (MQ=255) cTTCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAg < 1:4370050/71‑1 (MQ=255) ttCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCa < 1:1127012/67‑1 (MQ=255) ttCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGc > 1:4617235/1‑71 (MQ=255) cGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCg < 1:1531914/70‑1 (MQ=255) cGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGt > 1:1175034/1‑71 (MQ=255) cGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGt > 1:1475644/1‑71 (MQ=255) cGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACAGCCACGCCGCTGTCAAAGCGt > 1:5086699/1‑71 (MQ=255) cGCCGTACTCCTCTTTCGAAACATTAGTGATCGTGGCATAGCCACCCACCACCACGCCGCTATCAAAGCGt > 1:3441369/1‑71 (MQ=255) gCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGtt > 1:3353188/1‑71 (MQ=255) gCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGtt < 1:2128324/71‑1 (MQ=255) gCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGtt < 1:5532746/71‑1 (MQ=255) gCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGtt > 1:974424/1‑71 (MQ=255) gCCGTACTCCTCTTTCGAAACATTAGTGATCGTGGCATAGCCACCCACCACCACGCCGCTATCAAAGCGtt < 1:5330589/71‑1 (MQ=255) gCCGTACTCCTCTTTCGAAACATTAGTGATCGTGGCATAGCCACCCACCACCACGCCGCTATCAAAGCGtt < 1:2472515/71‑1 (MQ=255) gCCGTACTCCTCTTTCGAAACATTAGTGATCGTGGCATAGCCACCCACCACCACGCCGCTATCAAAGCGtt < 1:3947796/71‑1 (MQ=255) tAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGc < 1:480677/71‑1 (MQ=255) tAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGc < 1:4808028/71‑1 (MQ=255) tAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGc < 1:4142327/71‑1 (MQ=255) tCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGAc > 1:1802178/1‑71 (MQ=255) tCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGttt < 1:3959535/62‑1 (MQ=255) tttAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTgg > 1:4329797/1‑62 (MQ=255) tttAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTgg > 1:3438418/1‑62 (MQ=255) tAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCaaa > 1:4965837/1‑52 (MQ=255) tAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTc < 1:1132283/67‑1 (MQ=255) aGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATc < 1:1937831/70‑1 (MQ=255) aGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCg > 1:3990515/1‑71 (MQ=255) aCGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGt < 1:3173059/61‑1 (MQ=255) aCGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGt < 1:3184954/61‑1 (MQ=255) aCGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGt < 1:1915187/61‑1 (MQ=255) aCGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGt < 1:2120484/61‑1 (MQ=255) cGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGttt > 1:640864/1‑52 (MQ=255) aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGcc > 1:4071958/1‑69 (MQ=255) aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt > 1:1781640/1‑71 (MQ=255) aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCt < 1:5609738/70‑1 (MQ=255) aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCt < 1:5111406/70‑1 (MQ=255) cTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt > 1:3056408/1‑70 (MQ=255) aTTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATc < 1:5502744/71‑1 (MQ=255) aTTGCCGCCCATACCCCTACCGCCACGCCGCTGTAAAAGCGTTTGGCGCCg > 1:4320808/1‑51 (MQ=39) ttGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGc < 1:901283/46‑1 (MQ=255) gccgccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGa > 1:4972159/1‑46 (MQ=255) ccgccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCttt < 1:3106262/70‑1 (MQ=255) cgccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATc > 1:2368684/1‑52 (MQ=255) gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:1568474/1‑71 (MQ=255) gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:5199373/1‑71 (MQ=255) gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:3342361/1‑71 (MQ=255) cATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTg > 1:5674165/1‑67 (MQ=255) tACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTAt < 1:2749373/59‑1 (MQ=255) aCCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGAGCGTTGCGCCTTTATCTTTTGCCAGa > 1:1238703/1‑69 (MQ=255) ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt > 1:1812543/1‑62 (MQ=255) ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGatat > 1:2570779/1‑71 (MQ=255) ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGatat > 1:3008816/1‑71 (MQ=255) cTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGata > 1:4318178/1‑67 (MQ=255) tACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt > 1:5010922/1‑58 (MQ=255) tACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt > 1:3411183/1‑58 (MQ=255) accaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCtt > 1:891513/1‑49 (MQ=12) accaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGATACTGccc < 1:298773/71‑1 (MQ=255) ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGa < 1:326507/59‑1 (MQ=255) ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGa < 1:5241443/59‑1 (MQ=255) ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGATACTGccc > 1:5143422/4‑70 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt > 1:3179493/1‑48 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:5126942/1‑58 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc > 1:3446746/1‑70 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc > 1:561246/1‑70 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc < 1:881730/70‑1 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc > 1:1884513/1‑70 (MQ=255) cGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTa < 1:601751/49‑1 (MQ=255) ccACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCaa < 1:288315/69‑1 (MQ=255) cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc < 1:1653045/71‑1 (MQ=255) cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc < 1:459706/71‑1 (MQ=255) cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc < 1:90810/71‑1 (MQ=255) aCGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa < 1:3561650/57‑1 (MQ=255) cgccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa < 1:1531551/56‑1 (MQ=255) cgccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTa < 1:528289/71‑1 (MQ=255) gccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTa < 1:521562/70‑1 (MQ=255) gTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca > 1:1289911/1‑71 (MQ=255) | CGCCTTCGCCGTAGTCATCTTTAGATACGTTACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCA > minE/702724‑702861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |