| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 702,818 | T→G | 7.5% | A609A (GCA→GCC) ‡ | ymcA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 702,818 | 0 | T | G | 7.5% | 170.7 / 7.2 | 67 | A609A (GCA→GCC) ‡ | ymcA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (33/29); new base G (2/3); total (35/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
ACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTCGGCGG > minE/702755‑702886 | aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGcc > 1:4071958/1‑69 (MQ=255)aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt > 1:1781640/1‑71 (MQ=255)aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCt < 1:5111406/70‑1 (MQ=255)aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCt < 1:5609738/70‑1 (MQ=255) cTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt > 1:3056408/1‑70 (MQ=255) aTTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATc < 1:5502744/71‑1 (MQ=255) ccgccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCttt < 1:3106262/70‑1 (MQ=255) gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:3342361/1‑71 (MQ=255) gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:1568474/1‑71 (MQ=255) gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:5199373/1‑71 (MQ=255) cATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTg > 1:5674165/1‑67 (MQ=255) tACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTAt < 1:2749373/59‑1 (MQ=255) aCCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGAGCGTTGCGCCTTTATCTTTTGCCAGa > 1:1238703/1‑69 (MQ=255) ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt > 1:1812543/1‑62 (MQ=255) ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGatat > 1:2570779/1‑71 (MQ=255) ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGatat > 1:3008816/1‑71 (MQ=255) cTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGata > 1:4318178/1‑67 (MQ=255) tACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt > 1:5010922/1‑58 (MQ=255) tACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt > 1:3411183/1‑58 (MQ=255) accaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCtt > 1:891513/1‑49 (MQ=12) accaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGATACTGccc < 1:298773/71‑1 (MQ=255) ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGa < 1:5241443/59‑1 (MQ=255) ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGa < 1:326507/59‑1 (MQ=255) ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGATACTGccc > 1:5143422/4‑70 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt > 1:3179493/1‑48 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc > 1:5126942/1‑58 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc > 1:1884513/1‑70 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc < 1:881730/70‑1 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc > 1:3446746/1‑70 (MQ=255) ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc > 1:561246/1‑70 (MQ=255) cGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTa < 1:601751/49‑1 (MQ=255) ccACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCaa < 1:288315/69‑1 (MQ=255) cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc < 1:90810/71‑1 (MQ=255) cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc < 1:459706/71‑1 (MQ=255) cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc < 1:1653045/71‑1 (MQ=255) aCGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa < 1:3561650/57‑1 (MQ=255) cgccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa < 1:1531551/56‑1 (MQ=255) cgccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTa < 1:528289/71‑1 (MQ=255) gccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTa < 1:521562/70‑1 (MQ=255) gTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca > 1:1289911/1‑71 (MQ=255) tCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca < 1:2857177/70‑1 (MQ=255) aaaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtt > 1:4887726/1‑38 (MQ=255) aaaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGGCAGATATTGCCCAACGCTAAGTTtcat > 1:418394/1‑69 (MQ=255) aaaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCaa > 1:2892223/1‑56 (MQ=255) aaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAg < 1:1924502/71‑1 (MQ=255) aGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtt > 1:2909569/1‑36 (MQ=255) aGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtt > 1:4304310/1‑36 (MQ=255) cGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa > 1:2043584/1‑42 (MQ=255) cGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCAAGa > 1:1337801/1‑42 (MQ=255) tttGGCGACGTCGATCGTTGCGCCTTTATCTTTTGcc < 1:2275777/37‑1 (MQ=255) gACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTg < 1:2907826/70‑1 (MQ=255) cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc > 1:2266784/1‑71 (MQ=255) cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc < 1:730130/71‑1 (MQ=255) cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc > 1:1998746/1‑71 (MQ=255) cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc < 1:4467349/71‑1 (MQ=255) gTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca > 1:2535448/1‑53 (MQ=255) tCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtcat < 1:4353090/53‑1 (MQ=255) tCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca < 1:2084100/52‑1 (MQ=255) tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCGTTGAGCGTcggc > 1:457806/1‑71 (MQ=255) tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggc < 1:4804090/71‑1 (MQ=255) tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggc > 1:5518192/1‑71 (MQ=255) tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggc > 1:5626840/1‑71 (MQ=255) gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt < 1:2109904/42‑1 (MQ=255) gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt < 1:1431816/42‑1 (MQ=255) gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt < 1:675416/42‑1 (MQ=255) gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggcgg < 1:851888/71‑1 (MQ=255) gTTGCGCCTTTATCTTTT‑CCAGATATTGCCCAACGCTAAGt < 1:5359143/41‑1 (MQ=255) ttGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAg < 1:2352886/40‑1 (MQ=255) tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGttt > 1:5560009/1‑42 (MQ=255) tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGttt > 1:2662087/1‑42 (MQ=255) tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt < 1:1579802/40‑1 (MQ=255) tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTa > 1:3640279/1‑50 (MQ=255) | ACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTCGGCGG > minE/702755‑702886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |