| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 703,823 | C→G | 5.4% | R274R (CGG→CGC) | ymcA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 703,823 | 0 | C | G | 5.4% | 228.8 / 2.4 | 74 | R274R (CGG→CGC) | ymcA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (47/23); new base G (2/2); total (49/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01 | |||||||||||
TCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTG > minE/703755‑703889 | tCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg > 1:3250821/1‑71 (MQ=255)tCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg > 1:3616751/1‑71 (MQ=255)tCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg > 1:2219542/1‑71 (MQ=255)tCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg > 1:1480581/1‑71 (MQ=255)tCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg > 1:4865045/1‑71 (MQ=255) cAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg > 1:2775724/1‑70 (MQ=255) ggCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTaa > 1:4913628/1‑71 (MQ=255) cAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTg < 1:5627962/71‑1 (MQ=255) cAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTg < 1:2158183/71‑1 (MQ=255) aGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTg > 1:1840702/1‑70 (MQ=255) gTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg < 1:4982590/61‑1 (MQ=255) gTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg < 1:1885572/61‑1 (MQ=255) gTTGATATGCCAGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCg < 1:4358666/61‑1 (MQ=255) atatGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCa < 1:4355681/70‑1 (MQ=255) atatGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCa < 1:877951/70‑1 (MQ=255) atGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCaaa > 1:2177605/1‑70 (MQ=255) atGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCaaa > 1:5579130/1‑70 (MQ=255) ccGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTg < 1:4099818/59‑1 (MQ=255) ccGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTg < 1:394051/59‑1 (MQ=255) cGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATc > 1:1110835/1‑70 (MQ=255) cGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATc > 1:999812/1‑70 (MQ=255) cGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCa < 1:1662944/71‑1 (MQ=255) ggTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:109960/1‑71 (MQ=255) ggTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:3381006/1‑71 (MQ=255) tttGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGa > 1:4840384/1‑55 (MQ=255) tttGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGa > 1:2501632/1‑55 (MQ=255) gCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACg < 1:5654407/66‑1 (MQ=255) cGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTAc < 1:2031798/71‑1 (MQ=255) tATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGc < 1:2895887/51‑1 (MQ=255) tATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACgt < 1:2692703/64‑1 (MQ=255) tATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACgt < 1:2771650/64‑1 (MQ=255) tCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTaa > 1:5331475/1‑41 (MQ=255) tCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACcgcg > 1:5338065/1‑71 (MQ=255) gcgcAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGtt < 1:4107375/71‑1 (MQ=255) gcgcAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGtt < 1:2274816/71‑1 (MQ=255) cagggcagggCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt < 1:4726849/71‑1 (MQ=255) agggcagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt < 1:1171639/70‑1 (MQ=255) gggcagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATaa > 1:1433550/1‑71 (MQ=255) gggcagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATaa > 1:1789026/1‑71 (MQ=255) gacgggcGCAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATAAAGGTGTTGCCACGTTCATAGCt < 1:1149429/68‑1 (MQ=255) cagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACt > 1:5634674/1‑70 (MQ=255) acgggcGCAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATAAAGgtg > 1:3028354/3‑53 (MQ=255) agggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACt > 1:2925019/1‑69 (MQ=255) gggcGCAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATaaagg < 1:3648392/49‑3 (MQ=255) gcgcAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTAcc > 1:3580591/1‑54 (MQ=255) gcgcAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTAcc > 1:2101668/1‑54 (MQ=255) gcgcAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATAAAGGTGTTGCCACGTTCATAGCTAAGGt > 1:5142751/1‑71 (MQ=255) gcAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTg < 1:4706880/71‑1 (MQ=255) cAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACgtg < 1:3158008/46‑1 (MQ=255) aGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTgag < 1:3768694/71‑1 (MQ=255) gATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGtt < 1:2110083/46‑1 (MQ=255) gATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGtt > 1:1829395/1‑67 (MQ=255) gATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGtt > 1:2571267/1‑67 (MQ=255) aTCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGtt > 1:185409/1‑66 (MQ=255) aTCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACAGCGTTCATAACTCAGGtt > 1:5658889/1‑66 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:1864379/1‑37 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:628646/1‑37 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAAc > 1:921970/1‑38 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAAc > 1:3437851/1‑38 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:2300236/1‑62 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:1087217/1‑62 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:80547/1‑62 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgc > 1:76829/1‑61 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCt > 1:4514070/1‑71 (MQ=255) gTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:3238848/1‑65 (MQ=255) gTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:4265829/1‑65 (MQ=255) tGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGcc > 1:3591559/1‑71 (MQ=255) tGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGcc > 1:3713938/1‑71 (MQ=255) gAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:5049547/1‑62 (MQ=255) gAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:644927/1‑62 (MQ=255) gAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:2545410/1‑62 (MQ=255) tGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt > 1:2886105/1‑45 (MQ=255) tGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt > 1:3567367/1‑45 (MQ=255) tGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTg > 1:3031926/1‑71 (MQ=255) | TCAGGCGCAGGTTGATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTG > minE/703755‑703889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |