| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 703,834 | A→C | 5.3% | F271V (TTC→GTC) | ymcA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 703,834 | 0 | A | C | 5.3% | 198.7 / 4.1 | 76 | F271V (TTC→GTC) | ymcA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (45/27); new base C (2/2); total (47/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
ATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATAAAC > minE/703769‑703903 | atatGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCa < 1:877951/70‑1 (MQ=255)atatGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCa < 1:4355681/70‑1 (MQ=255) atGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCaaa > 1:5579130/1‑70 (MQ=255) atGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCaaa > 1:2177605/1‑70 (MQ=255) cGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATc > 1:999812/1‑70 (MQ=255) cGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATc > 1:1110835/1‑70 (MQ=255) cGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCa < 1:1662944/71‑1 (MQ=255) ggTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:109960/1‑71 (MQ=255) ggTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:3381006/1‑71 (MQ=255) gCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACg < 1:5654407/66‑1 (MQ=255) cGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTAc < 1:2031798/71‑1 (MQ=255) tATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGc < 1:2895887/51‑1 (MQ=255) tATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACgt < 1:2771650/64‑1 (MQ=255) tATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACgt < 1:2692703/64‑1 (MQ=255) tCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACcgcg > 1:5338065/1‑71 (MQ=255) gcgcAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGtt < 1:2274816/71‑1 (MQ=255) gcgcAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGtt < 1:4107375/71‑1 (MQ=255) cagggcagggCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt < 1:4726849/71‑1 (MQ=255) agggcagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt < 1:1171639/70‑1 (MQ=255) gggcagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATaa > 1:1789026/1‑71 (MQ=255) gggcagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATaa > 1:1433550/1‑71 (MQ=255) gacgggcGCAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATAAAGGTGTTGCCACGTTCATAGCt < 1:1149429/68‑1 (MQ=255) cagggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACt > 1:5634674/1‑70 (MQ=255) acgggcGCAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATAAAGgtg > 1:3028354/3‑53 (MQ=255) agggcGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACt > 1:2925019/1‑69 (MQ=255) gggcGCAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATaaagg < 1:3648392/49‑3 (MQ=255) gcgcAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTAcc > 1:3580591/1‑54 (MQ=255) gcgcAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTAcc > 1:2101668/1‑54 (MQ=255) gcgcAGATCGTTAAAGTTGGTGCGCAACGTAACGCCAAACATAAAGGTGTTGCCACGTTCATAGCTAAGGt > 1:5142751/1‑71 (MQ=255) gcAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTg < 1:4706880/71‑1 (MQ=255) cAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACgtg < 1:3158008/46‑1 (MQ=255) aGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTgag < 1:3768694/71‑1 (MQ=255) gATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGtt < 1:2110083/46‑1 (MQ=255) gATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGtt > 1:1829395/1‑67 (MQ=255) gATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGtt > 1:2571267/1‑67 (MQ=255) aTCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGtt > 1:185409/1‑66 (MQ=255) aTCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACAGCGTTCATAACTCAGGtt > 1:5658889/1‑66 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:1864379/1‑37 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCaa > 1:628646/1‑37 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAAc > 1:3437851/1‑38 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAAc > 1:921970/1‑38 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:80547/1‑62 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:1087217/1‑62 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:2300236/1‑62 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgc > 1:76829/1‑61 (MQ=255) cGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCt > 1:4514070/1‑71 (MQ=255) gTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:4265829/1‑65 (MQ=255) gTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:3238848/1‑65 (MQ=255) tGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGcc > 1:3713938/1‑71 (MQ=255) tGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGcc > 1:3591559/1‑71 (MQ=255) gAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:2545410/1‑62 (MQ=255) gAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:644927/1‑62 (MQ=255) gAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTga > 1:5049547/1‑62 (MQ=255) tGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt > 1:2886105/1‑45 (MQ=255) tGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt > 1:3567367/1‑45 (MQ=255) tGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTg > 1:3031926/1‑71 (MQ=255) cGTAACGTGAAGCCAAACATCAACTTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAgc > 1:2481980/1‑71 (MQ=255) cGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAgc > 1:1838828/1‑71 (MQ=255) tAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACt < 1:5390072/42‑1 (MQ=255) tAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAgcgc < 1:3595754/71‑1 (MQ=255) tAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAgcg > 1:4762669/1‑70 (MQ=255) aaCGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACt < 1:5655753/41‑1 (MQ=255) aCGTGAAGCCAAACATCAACGTGTTACCGCGTTCAt > 1:3557265/1‑36 (MQ=255) aCGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAgg > 1:2509495/1‑44 (MQ=255) aCGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGc > 1:4430949/1‑60 (MQ=255) aCGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGa > 1:5025772/1‑71 (MQ=255) aCGAGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAg > 1:2075463/1‑59 (MQ=255) gTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCtata < 1:4420229/71‑1 (MQ=255) gTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATa < 1:4395318/71‑1 (MQ=255) gTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATa < 1:599358/71‑1 (MQ=255) gTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATa < 1:1676637/71‑1 (MQ=255) gTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATa < 1:877608/71‑1 (MQ=255) gTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATa < 1:4676954/71‑1 (MQ=255) tGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATaa < 1:3476720/71‑1 (MQ=255) tGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATaa < 1:853949/71‑1 (MQ=255) gAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATaaa > 1:254318/1‑71 (MQ=255) aaGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATaaa < 1:4267328/70‑1 (MQ=255) aaGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATaaa < 1:239660/70‑1 (MQ=255) aaGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATAAAc > 1:3654422/1‑71 (MQ=255) | ATATGCCGGTTTTGGCGTATCGCGCAGGGCAGGGCGCAGATCGTTGAAATTGGTCCGTAACGTGAAGCCAAACATCAACGTGTTACCGCGTTCATAACTCAGGTTGAGATCTGCCCAGCTGGCAGCGCGATAAAC > minE/703769‑703903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |