| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 704,303 | A→C | 22.2% | L114L (CTT→CTG) | ymcA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 704,303 | 0 | A | C | 22.2% | 164.3 / 58.1 | 99 | L114L (CTT→CTG) | ymcA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (42/35); new base C (10/12); total (52/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTAT > minE/704238‑704367 | gTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGtt > 1:4853548/1‑70 (MQ=255) ccAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGaa > 1:1735024/1‑64 (MQ=255) aGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGaa > 1:2754284/1‑62 (MQ=255) gCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATc < 1:1958778/71‑1 (MQ=255) atCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:2059709/1‑69 (MQ=255) atCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:4044390/1‑69 (MQ=255) tCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:5641687/61‑1 (MQ=255) tCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAAt > 1:4654760/1‑69 (MQ=255) aCGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:2205433/1‑62 (MQ=255) aCGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAtt > 1:4414167/1‑71 (MQ=255) aCGTTTACCAAACGCAACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:3409610/1‑62 (MQ=255) gTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTg > 1:2473338/1‑71 (MQ=255) tttACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGa < 1:2828585/49‑1 (MQ=255) ttACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaaa > 1:311831/1‑57 (MQ=255) tACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAttt < 1:4087923/67‑1 (MQ=255) aCCAACCGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCtt < 1:5372011/71‑1 (MQ=255) ccAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCtt < 1:2763889/70‑1 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaaa > 1:3623456/1‑53 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAttt < 1:4834842/64‑1 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:4194489/71‑1 (MQ=255) cAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:2303699/71‑1 (MQ=255) aaaCGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGt < 1:5401449/71‑1 (MQ=255) accgcgACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTtata > 1:4471478/3‑71 (MQ=255) accGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTtata > 1:3816527/3‑71 (MQ=255) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:2811956/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:5020055/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:1650483/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:4133729/45‑1 (MQ=16) ccGCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt < 1:1437401/45‑1 (MQ=16) cGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGATTGATTTGTCTTTGTATg < 1:2826470/71‑1 (MQ=255) gCCACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGttt > 1:3198555/1‑44 (MQ=16) ccACTTGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGtt > 1:5680876/1‑42 (MQ=16) aCTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTg < 1:298368/71‑1 (MQ=255) cTTGCGGTAGCCAGTAACCTTCTTCCCAGAGGCGAAGttt > 1:5490348/1‑40 (MQ=255) cTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGttt > 1:5004219/1‑40 (MQ=255) cTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGa < 1:4845245/71‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaa < 1:603560/42‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaaa < 1:4581591/42‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:279047/41‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:1533551/41‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTaa < 1:2062117/41‑1 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt > 1:2282672/1‑60 (MQ=255) ttGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt > 1:1376976/1‑60 (MQ=255) ttGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATc < 1:4057529/57‑1 (MQ=255) tGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCt > 1:3091942/1‑57 (MQ=255) tGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATc > 1:1750856/1‑71 (MQ=255) tGCGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATc > 1:3675203/1‑71 (MQ=255) tGCGGCAGCCAGTAACTCACTTCCCACAAACGCAGATTGAGATCGAAGGCTTTATCTTtata > 1:3758542/1‑62 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:570752/1‑42 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:4923259/1‑42 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAAt > 1:5088302/1‑42 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:1240711/1‑46 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGaa > 1:3399473/1‑46 (MQ=255) gCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATcg > 1:2796996/1‑71 (MQ=255) cGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATcgc < 1:4096640/71‑1 (MQ=255) cGGCAGCCAGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCTTTATACGTTTGATcgc < 1:3651759/71‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:1648098/55‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:4445834/55‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:2474168/55‑1 (MQ=255) tAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTg < 1:1210079/55‑1 (MQ=255) aGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCttta > 1:1501238/1‑49 (MQ=255) aGTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCTTTATCttta > 1:1135682/1‑49 (MQ=255) aGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGaaa < 1:2124752/71‑1 (MQ=255) aGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGaaa < 1:2746868/71‑1 (MQ=255) gTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCttta < 1:5629868/43‑2 (MQ=255) gTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCttta < 1:4888498/43‑2 (MQ=255) gTAACTCTCTTCCCACAAACGCAGTTTGAGATCGAAGGCttta < 1:3257370/43‑2 (MQ=255) gTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAAt < 1:3557014/71‑1 (MQ=255) gTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCAGAAAt < 1:2269246/71‑1 (MQ=255) tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt < 1:5454464/47‑1 (MQ=255) tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt < 1:4772255/47‑1 (MQ=255) tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCttt < 1:1188925/47‑1 (MQ=255) tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGaa > 1:1528892/1‑68 (MQ=255) tAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGaa > 1:130526/1‑68 (MQ=255) aaCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACt > 1:3500166/1‑54 (MQ=255) aaCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACt > 1:1236879/1‑54 (MQ=255) aaCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCt > 1:1163262/1‑71 (MQ=255) aCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGAttt > 1:2115823/1‑39 (MQ=255) aCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGt > 1:5538718/1‑47 (MQ=255) aCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCtt > 1:1807894/1‑71 (MQ=255) aCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCtt > 1:3100519/1‑71 (MQ=255) aCCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCt > 1:4132175/1‑70 (MQ=255) cttcttCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTcc > 1:4450791/1‑71 (MQ=255) ttcttcCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAAt > 1:3540141/1‑65 (MQ=255) ttcttcCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCAGCTGAAATCTTcc > 1:2706530/1‑70 (MQ=255) tcttcCCACAGGCGAAGTTTAAAATCGAGTGAttt < 1:2149124/35‑1 (MQ=255) cttcCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCAc > 1:4357608/1‑71 (MQ=255) cttcCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCAc > 1:3531913/1‑71 (MQ=255) ttcCCACAGGCGAATTTTAAAATCGAATGATTTGTCTTTg < 1:1648101/40‑1 (MQ=255) acaGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTa > 1:822542/1‑37 (MQ=255) acaGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTAt < 1:2463480/38‑1 (MQ=255) acaGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGaa < 1:4293144/55‑1 (MQ=255) acaGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCta > 1:428365/1‑71 (MQ=255) caGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGGTGAAATCTTCCCACTGGc < 1:4277656/68‑1 (MQ=255) caGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAAt > 1:875528/1‑56 (MQ=255) caGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCtat < 1:1734292/71‑1 (MQ=255) cGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCt > 1:2227201/1‑47 (MQ=255) cGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCt > 1:1814948/1‑47 (MQ=255) gAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGa > 1:3783593/1‑38 (MQ=255) aaGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAAt < 1:5651640/50‑1 (MQ=255) | GTACCAGCAATATCACGTTTACCAAACGCCACTTGCGGTAGCCAGTAACCTTCTTCCCACAGGCGAAGTTTAAAATCGAATGATTTGTCTTTGTATGACTGATCGCCGCTGAAATCTTCCCACTGGCTAT > minE/704238‑704367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |