Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	821,445	T→C	75.0%	S7G (AGC→GGC)	ldrA ←	toxic polypeptide, small

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	821,445	0	T	C	75.0%	67.3 / 22.4	44	S7G (AGC→GGC)	ldrA	toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base T (2/9);  new base C (18/15);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 4.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTA  >  minE/821395‑821506                                                   |                                                              cGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGt                                           >  1:1416822/1‑71 (MQ=255) cGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGt                                           >  1:4303630/1‑71 (MQ=255)  gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          <  1:4339373/71‑1 (MQ=255)  gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          <  1:1710798/71‑1 (MQ=255)  gCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          <  1:2501277/71‑1 (MQ=255)   ccaccaGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCa                                         <  1:3524084/71‑1 (MQ=34)    caccaGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCAt                                        >  1:1216590/1‑71 (MQ=255)     accaGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATg                                       >  1:4371520/1‑71 (MQ=255)      ccaGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATgg                                      >  1:5172359/1‑71 (MQ=34)        aGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCa                                    <  1:3448904/71‑1 (MQ=34)        aGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGc                                     >  1:647017/1‑70 (MQ=255)        aGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCa                                    <  1:4313762/71‑1 (MQ=255)          cTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCaa                                   <  1:1627951/70‑1 (MQ=35)                  cGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgcgc                            >  1:241570/1‑69 (MQ=25)                   gCTGCGGTAATAATTCCCGCCAGGATCGTTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAg                         >  1:2195765/1‑71 (MQ=255)                   gCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCa                                         >  1:2356212/1‑55 (MQ=35)                   gCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGa                          <  1:2454213/70‑1 (MQ=25)                      gCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACt                                >  1:5638646/1‑61 (MQ=25)                       cGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTc                     <  1:4204334/71‑1 (MQ=34)                       cGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCg                       <  1:3076164/69‑1 (MQ=255)                        ggTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa                    <  1:4023875/71‑1 (MQ=255)                         gTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCat                   <  1:5586855/71‑1 (MQ=255)                          taataaTTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  <  1:1851649/71‑1 (MQ=255)                           aataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  >  1:2923647/1‑70 (MQ=255)                           aataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  >  1:2321353/1‑70 (MQ=255)                            ataatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCata                  >  1:4554846/1‑69 (MQ=255)                             taatTCCCGCCAGGATCGGTGCTGCCAGTTCGTGCCAGAAAGTCATGGCAAACTgc                              <  1:149218/56‑1 (MQ=255)                             taatTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACt                                <  1:2683247/54‑1 (MQ=35)                             taatTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgc                              <  1:5047611/56‑1 (MQ=35)                             taatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTgcgc                            <  1:1263092/58‑1 (MQ=255)                             taatTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAg               >  1:55659/1‑71 (MQ=21)                                ttCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATg                                       <  1:1596578/44‑1 (MQ=255)                                ttCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCaaa                                  >  1:4809753/1‑49 (MQ=255)                                  cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          >  1:5463922/1‑39 (MQ=255)                                  cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          >  1:1615482/1‑39 (MQ=255)                                  cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTc                                          >  1:4940680/1‑39 (MQ=255)                                   ccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCaaa                                  >  1:1809766/1‑46 (MQ=255)                                     gCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg       <  1:3886981/71‑1 (MQ=34)                                     gCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttg       <  1:2042218/71‑1 (MQ=34)                                       cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGTCAAACTGCGCGAGCGTCa                    <  1:3270180/56‑1 (MQ=11)                                       cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa                    <  1:4504964/56‑1 (MQ=255)                                       cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCa                    <  1:1160594/56‑1 (MQ=255)                                        aGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCttgttg    >  1:5482186/1‑71 (MQ=21)                                               gTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTa  <  1:4394382/66‑1 (MQ=25)                                                   |                                                              CGCCACCAGCTGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCTGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTA  >  minE/821395‑821506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑