| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,565 | T→A | 19.0% | V354V (GTT→GTA) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,565 | 0 | T | A | 19.0% | 139.8 / 27.6 | 63 | V354V (GTT→GTA) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (25/26); new base A (5/7); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
CCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCT > minE/189498‑189633 | ccTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTg < 1:4371691/69‑1 (MQ=255) cATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:208090/71‑1 (MQ=255) aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa > 1:4794873/1‑70 (MQ=255) aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa > 1:4125926/1‑70 (MQ=255) ttttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCg < 1:4439234/68‑1 (MQ=255) tttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaat > 1:1807745/1‑70 (MQ=255) ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACg > 1:5517853/1‑71 (MQ=255) cATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc < 1:4558411/70‑1 (MQ=255) aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa > 1:3819833/1‑71 (MQ=255) aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa > 1:1999617/1‑71 (MQ=255) aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa > 1:3298468/1‑71 (MQ=255) tCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAAt < 1:1594876/71‑1 (MQ=255) accGATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAAccc < 1:33132/67‑3 (MQ=255) ccGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:1319715/1‑71 (MQ=255) ccGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:4838050/1‑71 (MQ=255) cGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc > 1:3192380/1‑70 (MQ=255) gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGAt < 1:5542532/54‑1 (MQ=255) gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGAt < 1:4494528/54‑1 (MQ=255) gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTcca < 1:2364591/71‑3 (MQ=255) gATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:3906376/1‑71 (MQ=255) acTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc > 1:5670592/2‑71 (MQ=255) aCCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCca > 1:161151/1‑70 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc < 1:1944296/63‑1 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:1068464/1‑65 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa > 1:5037802/1‑65 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcaca < 1:4386824/71‑1 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:365569/1‑70 (MQ=255) ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac > 1:3364452/1‑70 (MQ=255) gaaggTAAATGCGGTATGAGCGGTGTCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaa < 1:2288477/68‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:731261/53‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:360930/53‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:2448136/53‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:2804454/53‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:3126184/53‑1 (MQ=255) cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa < 1:1677336/71‑1 (MQ=255) aaggTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaaa > 1:3386987/3‑71 (MQ=255) ggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt < 1:4932725/71‑1 (MQ=255) tAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGc < 1:4873280/71‑1 (MQ=255) tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc < 1:1186021/53‑1 (MQ=255) tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc < 1:4392755/53‑1 (MQ=255) tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc < 1:4177264/53‑1 (MQ=255) tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc < 1:4325413/53‑1 (MQ=255) aaaaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCt > 1:1720803/1‑71 (MQ=255) aaaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCtt > 1:250502/1‑43 (MQ=255) aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg > 1:4387942/1‑71 (MQ=255) aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg < 1:1277332/71‑1 (MQ=255) tGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa > 1:1664180/1‑62 (MQ=255) cggTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaa > 1:4637517/2‑60 (MQ=255) cggTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACACAGTCCACTCACaa > 1:808938/2‑60 (MQ=255) gTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGgcggcg > 1:765353/1‑71 (MQ=255) aTGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt > 1:3310451/1‑71 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:1044632/37‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:3555802/37‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa < 1:5656078/37‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGtt < 1:4972451/70‑1 (MQ=255) tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa < 1:4594300/71‑1 (MQ=255) gAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt < 1:3147560/69‑1 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaa > 1:2804721/1‑36 (MQ=255) aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaa > 1:2471900/1‑36 (MQ=255) gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg < 1:5024137/71‑1 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:5478784/1‑71 (MQ=255) gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg > 1:1361244/1‑71 (MQ=255) tgtTGCGGGAAAAGAGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa < 1:4531633/63‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt < 1:193018/43‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATTCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1891243/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:1688458/70‑1 (MQ=255) ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt < 1:4195592/70‑1 (MQ=255) | CCTGGGTGCCGTACACCCATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCT > minE/189498‑189633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |