| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,605 | T→C | 15.2% | intergenic (‑142/+312) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,605 | 0 | T | C | 15.2% | 90.1 / 11.0 | 46 | intergenic (‑142/+312) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (22/17); new base C (3/4); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
GTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGT > minE/821537‑821675 | ttCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTc < 1:2163854/69‑1 (MQ=11) gTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGc > 1:1714470/1‑70 (MQ=35) gttCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGc < 1:4008734/68‑1 (MQ=255) ttCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAc < 1:4248633/71‑1 (MQ=21) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc > 1:2561353/1‑70 (MQ=35) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:848013/70‑1 (MQ=35) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:1348151/70‑1 (MQ=35) aaGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCa < 1:3345401/70‑1 (MQ=25) gATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATg > 1:2076834/1‑71 (MQ=34) gATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATg < 1:2913665/71‑1 (MQ=21) aTTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGc > 1:2321658/1‑71 (MQ=21) aTTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGc < 1:2163303/71‑1 (MQ=21) tAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca > 1:1831334/1‑71 (MQ=21) tAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcca > 1:2765249/1‑71 (MQ=21) gCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGccac < 1:1062496/70‑1 (MQ=35) gTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGTGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGa > 1:4432181/1‑70 (MQ=255) ttCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGAt < 1:4036196/70‑1 (MQ=25) ttCTGTTGTAAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCtt > 1:863469/1‑45 (MQ=255) tCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAc > 1:5281153/1‑53 (MQ=16) cTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATa > 1:559051/1‑69 (MQ=14) cTGTTGTCAGGTTGTACCTATCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGc > 1:205662/1‑58 (MQ=11) ggTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCa < 1:4729267/71‑1 (MQ=25) aggtttACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGc < 1:1827786/67‑1 (MQ=25) ttgtACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAAc < 1:2443606/68‑1 (MQ=255) gtACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACa < 1:5293143/69‑1 (MQ=255) ctctCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTgc > 1:4618189/1‑70 (MQ=40) ctcAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGcc > 1:5364258/1‑71 (MQ=39) cAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCt > 1:829715/1‑70 (MQ=40) cAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCt > 1:3491571/1‑70 (MQ=40) cAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCt > 1:4857575/1‑70 (MQ=40) aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTc < 1:2141498/70‑1 (MQ=40) aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCa > 1:613276/1‑71 (MQ=39) aaCGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCa < 1:1142705/71‑1 (MQ=39) aaCGTGCGGGGGTTTTCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCa > 1:4978412/1‑71 (MQ=255) aCGTGCGGGGGTTTTCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCAc > 1:216469/1‑71 (MQ=255) gTGCGGGGGTTTTCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCg > 1:4465944/1‑71 (MQ=255) gggTTTTCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGa < 1:1113653/67‑1 (MQ=255) ttttCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTgcgc < 1:708275/55‑1 (MQ=255) ttttCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa < 1:2124949/47‑1 (MQ=255) ttttCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa < 1:3128091/47‑1 (MQ=255) ttttCTCTCTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa < 1:3003723/47‑1 (MQ=255) ttCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCgg > 1:3857988/1‑71 (MQ=255) tctcTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGa > 1:4914605/1‑61 (MQ=40) ctctctCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa < 1:124957/43‑1 (MQ=255) ctctctCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa < 1:73328/43‑1 (MQ=255) ctctTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACAt > 1:2605328/1‑46 (MQ=255) ctctTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCAcc > 1:4710655/1‑57 (MQ=255) ctctTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCAcc > 1:2319123/1‑57 (MQ=255) tctTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTgcg > 1:3196446/1‑49 (MQ=255) tttCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCAc > 1:5163547/1‑53 (MQ=255) tttCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCt < 1:4219710/68‑1 (MQ=255) ttCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGgt > 1:1874567/1‑71 (MQ=255) | GTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGT > minE/821537‑821675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |