| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,688 | T→A | 21.0% | intergenic (‑225/+229) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,688 | 0 | T | A | 21.0% | 127.6 / 31.1 | 62 | intergenic (‑225/+229) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (19/30); new base A (4/9); total (23/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAC > minE/821621‑821752 | caccaGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGtct < 1:3694624/70‑1 (MQ=255) gggATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTc < 1:3247632/71‑1 (MQ=255) gggATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTc < 1:1960772/71‑1 (MQ=255) gATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCAt > 1:3753573/1‑71 (MQ=255) aTAAAGCCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATc > 1:420335/1‑71 (MQ=11) gCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATcttcac > 1:5298653/1‑71 (MQ=255) ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATcttcactt < 1:5222463/69‑1 (MQ=255) ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATctt < 1:5470837/64‑1 (MQ=255) ccGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATctt < 1:357918/64‑1 (MQ=255) cGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCAt > 1:851332/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATcc > 1:1706426/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATcc < 1:4355330/71‑1 (MQ=255) aaCATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTAACTTCATccc < 1:2057318/71‑1 (MQ=255) aCATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGTATACTACGt > 1:4644459/1‑46 (MQ=255) cATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCt > 1:4058878/1‑70 (MQ=255) cATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCt > 1:5251838/1‑70 (MQ=255) cATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCt > 1:3173459/1‑70 (MQ=255) aTTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCaa < 1:3422698/49‑1 (MQ=255) gcCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:1405894/71‑1 (MQ=255) gcCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:5009579/71‑1 (MQ=255) aCCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:5444792/70‑1 (MQ=255) aCCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa < 1:858252/71‑1 (MQ=255) cGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa < 1:3770626/70‑1 (MQ=255) cGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa < 1:2101632/70‑1 (MQ=255) ggATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATa < 1:304988/71‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGaa < 1:1160941/58‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGaa < 1:1308997/58‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGa < 1:5187130/57‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGa < 1:233630/57‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATa > 1:2825760/1‑70 (MQ=255) tAATTCCGGCTTGGTGTGGATACTACGTCTCAAtt > 1:1965953/1‑35 (MQ=255) tAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAAt > 1:2051320/1‑58 (MQ=255) aTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATcttcacttc < 1:2722744/45‑1 (MQ=255) aTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATcttcacttc < 1:1247612/45‑1 (MQ=255) aTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATcttcacttc < 1:147074/45‑1 (MQ=255) aTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAag < 1:2725243/69‑1 (MQ=255) ttCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt < 1:1147776/59‑1 (MQ=40) ttCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt < 1:3641226/59‑1 (MQ=40) ttCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa < 1:4671516/63‑1 (MQ=39) cGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGttt < 1:5044334/56‑1 (MQ=255) gCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAtg < 1:1363072/60‑2 (MQ=255) tGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGaaa > 1:2147106/1‑46 (MQ=255) tGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGaaa > 1:2633534/1‑46 (MQ=255) ggTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCgg > 1:2642846/1‑71 (MQ=39) tgtgGATACTACGACTCAATTCATCTTCACTTCATCCCTGa < 1:807495/41‑1 (MQ=255) tgtgGATACTACGACTCAATTCATCTTCACTTCATCCCTGa < 1:4514396/41‑1 (MQ=255) tgtgGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:2035275/50‑1 (MQ=255) tgtgGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:3590591/50‑1 (MQ=255) tgtgGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:5193936/50‑1 (MQ=255) tgGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATg < 1:2183216/43‑1 (MQ=255) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:1104757/1‑70 (MQ=40) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:5258918/71‑1 (MQ=39) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:5623243/71‑1 (MQ=39) gATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt > 1:4679074/1‑42 (MQ=255) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:3600496/1‑42 (MQ=38) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:831210/1‑42 (MQ=38) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:3998554/1‑71 (MQ=39) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:3703521/1‑71 (MQ=39) actacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:4528678/1‑70 (MQ=40) ctacGTCTCAATTCATCTTCACTTCATCCCTGAAATGtt < 1:731949/39‑1 (MQ=40) ctacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAc < 1:1392233/70‑1 (MQ=40) ctacGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:1785173/42‑1 (MQ=255) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa > 1:4662579/1‑50 (MQ=255) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa > 1:359793/1‑50 (MQ=255) | CACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAC > minE/821621‑821752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |