| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,728 | A→G | 24.6% | intergenic (‑265/+189) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,728 | 0 | A | G | 24.6% | 139.5 / 40.4 | 73 | intergenic (‑265/+189) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base A (25/30); new base G (9/9); total (34/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
GGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCT > minE/821658‑821791 | ggATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATa < 1:304988/71‑1 (MQ=255) gATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATa > 1:2825760/1‑70 (MQ=255) aTTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAag < 1:2725243/69‑1 (MQ=255) ggTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCgg > 1:2642846/1‑71 (MQ=39) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:1104757/1‑70 (MQ=40) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:5623243/71‑1 (MQ=39) gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:5258918/71‑1 (MQ=39) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:3703521/1‑71 (MQ=39) tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:3998554/1‑71 (MQ=39) actacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:4528678/1‑70 (MQ=40) ctacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAc < 1:1392233/70‑1 (MQ=40) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa > 1:359793/1‑50 (MQ=255) cGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa > 1:4662579/1‑50 (MQ=255) cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATaaa > 1:656551/1‑40 (MQ=40) cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATaaa > 1:4800916/1‑40 (MQ=40) aaTTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTg < 1:2788131/69‑1 (MQ=35) tCATCTTCACTTCATCCCTGAAATGTTTGCAATaa > 1:3548618/1‑35 (MQ=37) tCATCTTCACTTCATCCCTGAAATGTTTGCAATaa > 1:377284/1‑35 (MQ=37) tCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa < 1:703983/41‑1 (MQ=38) tCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa < 1:3211241/41‑1 (MQ=38) cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:4463368/1‑53 (MQ=35) cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt > 1:2354029/1‑53 (MQ=35) cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACa > 1:3531416/1‑58 (MQ=35) cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc > 1:636252/1‑60 (MQ=35) aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTa < 1:1923710/39‑1 (MQ=40) aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt < 1:3024025/71‑1 (MQ=39) ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:3937129/1‑69 (MQ=40) ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt < 1:4887246/68‑1 (MQ=40) ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt > 1:320400/1‑69 (MQ=255) tcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:576134/70‑1 (MQ=255) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCa > 1:12353/1‑70 (MQ=11) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTcc > 1:797557/1‑40 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGttt < 1:1446410/68‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:766275/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:4797940/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:2007512/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCa > 1:2046808/1‑70 (MQ=255) cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt > 1:2184858/1‑71 (MQ=255) acttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:5603295/70‑1 (MQ=11) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:523176/70‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:4844769/70‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:2873495/70‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg < 1:2383303/71‑1 (MQ=255) acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg < 1:216736/71‑1 (MQ=255) cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc < 1:5234717/67‑1 (MQ=40) cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGa < 1:1444423/71‑1 (MQ=255) caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGa > 1:1055425/1‑68 (MQ=11) caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTg > 1:3842299/1‑71 (MQ=14) aTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGc < 1:1585178/71‑1 (MQ=255) tCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt < 1:4156454/64‑1 (MQ=255) cccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct > 1:5006544/1‑70 (MQ=255) ccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc > 1:83291/1‑45 (MQ=38) ccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc > 1:5061367/1‑45 (MQ=38) cTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:4718052/71‑1 (MQ=17) tGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc < 1:1754790/37‑1 (MQ=40) gAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga > 1:2975512/1‑71 (MQ=17) gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc > 1:2800009/1‑67 (MQ=255) gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc > 1:3716784/1‑67 (MQ=255) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt < 1:1047272/58‑1 (MQ=11) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat < 1:976120/71‑1 (MQ=17) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat < 1:2869499/71‑1 (MQ=17) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa > 1:5271687/1‑37 (MQ=40) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTgg < 1:4647999/69‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:3052047/71‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:5206207/71‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat < 1:3996150/71‑1 (MQ=255) aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGga > 1:807401/1‑70 (MQ=255) aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg < 1:3749695/71‑1 (MQ=18) tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc > 1:385316/1‑71 (MQ=18) ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg > 1:4706826/1‑66 (MQ=18) ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt > 1:1955198/1‑71 (MQ=18) ttGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc > 1:2712986/1‑41 (MQ=38) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag > 1:4322892/1‑66 (MQ=18) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag > 1:5250076/1‑66 (MQ=18) tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt < 1:2690654/70‑1 (MQ=18) tGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTg > 1:4844364/1‑56 (MQ=255) aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:1423380/58‑1 (MQ=17) aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg < 1:4827439/58‑1 (MQ=17) aTAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc < 1:4906049/36‑1 (MQ=40) aaaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg > 1:2203093/1‑36 (MQ=33) | GGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCT > minE/821658‑821791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |