| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,826 | A→T | 44.4% | intergenic (‑363/+91) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,826 | 0 | A | T | 44.4% | 14.7 / 57.9 | 45 | intergenic (‑363/+91) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base A (9/16); new base T (8/12); total (17/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCA > minE/821760‑821891 | gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:1084642/70‑1 (MQ=255)gCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGAt < 1:3781259/70‑1 (MQ=255) cAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATa < 1:2591327/70‑1 (MQ=255) cAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATa < 1:5597983/70‑1 (MQ=255) tCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:2494345/70‑1 (MQ=255) aTGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaaa < 1:4944308/70‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1054012/71‑1 (MQ=255) ctctGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:1508998/71‑1 (MQ=255) ctGGATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:1141625/59‑1 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGAc > 1:34747/1‑55 (MQ=255) tGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa < 1:2228891/68‑1 (MQ=255) ggATGAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGc > 1:2077133/1‑70 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCa > 1:4257911/1‑66 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg < 1:4582793/68‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAgg < 1:2680119/68‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGc < 1:3805761/69‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCt < 1:3346073/70‑1 (MQ=255) gatgatGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTg > 1:2476154/1‑71 (MQ=255) gatgagGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCt < 1:2602855/70‑1 (MQ=255) atgaggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt < 1:354236/71‑1 (MQ=255) aggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:5235309/50‑1 (MQ=255) aggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACaaa < 1:4533099/50‑1 (MQ=255) tgCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGc < 1:4883770/57‑1 (MQ=255) gCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTAccc < 1:2757126/71‑1 (MQ=255) gCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa > 1:666443/1‑44 (MQ=255) cTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTAcccc > 1:2328042/1‑71 (MQ=255) cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa > 1:1169744/1‑39 (MQ=255) cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACaaaa > 1:3300256/1‑46 (MQ=255) cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc < 1:2159948/70‑1 (MQ=255) cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGc < 1:4517571/70‑1 (MQ=255) cAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCg > 1:5073868/1‑71 (MQ=255) aGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTAc > 1:3388011/1‑63 (MQ=255) gCTCGGGTTGCCAATATTTACTTGTGGAAGAGATaaa < 1:4728784/37‑1 (MQ=255) tCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCg > 1:1938616/1‑71 (MQ=255) ggTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGtt > 1:4949673/1‑71 (MQ=255) gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCt < 1:4833049/51‑1 (MQ=255) gTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCt < 1:1016865/51‑1 (MQ=255) tGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGt > 1:3390871/1‑51 (MQ=255) gCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCg > 1:3036666/1‑60 (MQ=255) gCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCgg > 1:263875/1‑71 (MQ=255) tatTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCa < 1:218579/71‑1 (MQ=255) tttACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGt > 1:4858454/1‑59 (MQ=255) gtgGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCAt > 1:5444806/1‑71 (MQ=255) gAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCa < 1:5211129/70‑1 (MQ=255) aaGTGATAATGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGTGCGCATATTGCCATc < 1:901305/68‑1 (MQ=14) agaTAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCa > 1:253090/1‑66 (MQ=255) | GCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGAGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCA > minE/821760‑821891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |