| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 822,224 | G→T | 18.0% | intergenic (‑200/+204) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 822,224 | 0 | G | T | 18.0% | 327.1 / 39.8 | 122 | intergenic (‑200/+204) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base G (42/58); new base T (11/11); total (53/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
ACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTT > minE/822157‑822293 | accaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa < 1:599951/71‑1 (MQ=255) ccaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGTATACTACGTCGCAAt < 1:3480473/71‑1 (MQ=255) ccaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt > 1:1379073/1‑71 (MQ=255) aGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc < 1:4451800/71‑1 (MQ=255) ggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:319644/1‑70 (MQ=255) gATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc < 1:886652/71‑1 (MQ=255) aaGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:5087104/1‑71 (MQ=255) ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt < 1:83780/71‑1 (MQ=255) cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCAt > 1:1714408/1‑71 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc > 1:1394750/1‑57 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa < 1:5676508/58‑1 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATc > 1:4467792/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt < 1:2390254/54‑1 (MQ=255) cATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTg < 1:1168377/71‑1 (MQ=255) ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGa > 1:3643404/1‑70 (MQ=255) gcgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAAt > 1:4371855/1‑71 (MQ=255) gcgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGaaa < 1:4268317/70‑1 (MQ=255) gcgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAAt > 1:2072270/1‑71 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc < 1:5627899/48‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAAt < 1:2088267/70‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAAt < 1:5077101/70‑1 (MQ=255) cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCAt > 1:4101863/1‑58 (MQ=255) cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGt < 1:1248916/69‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc < 1:3699999/39‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc < 1:4055554/39‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaaa < 1:5540844/65‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:3794510/1‑71 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:406941/1‑68 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGttt > 1:21360/1‑68 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:4915399/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:178178/70‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttc > 1:5689103/1‑48 (MQ=11) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttc > 1:2872262/1‑48 (MQ=11) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttc > 1:86823/1‑48 (MQ=11) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:2789590/66‑1 (MQ=255) ccGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:3472988/1‑71 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:426880/1‑37 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:4758432/1‑37 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:3772991/1‑37 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:3074835/1‑37 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt < 1:3962130/71‑1 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt < 1:5287372/71‑1 (MQ=255) ggATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt > 1:5511009/1‑70 (MQ=255) ggATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATg < 1:4985707/71‑1 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:4295043/1‑45 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:3820851/1‑45 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa < 1:4864621/71‑1 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGa < 1:2656693/71‑1 (MQ=255) aTAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa < 1:4759336/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttc < 1:4876622/42‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:3339440/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:2377871/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:1151289/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATAC‑ACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:1681164/1‑65 (MQ=11) aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt > 1:2747774/1‑45 (MQ=255) aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTAATCCCTGAAATg > 1:3432142/1‑58 (MQ=255) aCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag < 1:1366693/71‑1 (MQ=255) ccGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTa > 1:4998389/1‑70 (MQ=40) cGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:4686396/1‑37 (MQ=40) cGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:4099330/1‑37 (MQ=40) cGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:291000/1‑37 (MQ=40) ggCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCAGAAATGTTTGCAATGAaga > 1:2568887/1‑65 (MQ=255) cTTGGTGTGGATACTACGTCGCAATTCATCTGCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTc < 1:295500/71‑1 (MQ=255) tGGTGTGGATACTACGTCGCAATTCATcttcacttca > 1:436697/1‑37 (MQ=40) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:4335476/1‑71 (MQ=39) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:755966/1‑71 (MQ=39) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg < 1:5155005/70‑1 (MQ=40) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg < 1:2021548/70‑1 (MQ=40) gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:4311928/56‑1 (MQ=255) gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:103331/56‑1 (MQ=255) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt < 1:1886170/69‑1 (MQ=40) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt < 1:3742314/69‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGTCTTTTc < 1:3874844/71‑1 (MQ=255) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt < 1:1141021/70‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:2363604/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:3260012/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:1493152/71‑1 (MQ=39) tactacGTCGCAATTCATCTTCACTTCATCCCTGa < 1:1658343/35‑1 (MQ=40) tactacGTCGCAATTCATCTTCACTTCATCCCTGa < 1:2615220/35‑1 (MQ=40) tactacGTCGCAATTCATCTTCACTTCATCCCTGa < 1:5367366/35‑1 (MQ=40) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:360394/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2521769/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2034085/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2261381/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:3563197/1‑70 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATg < 1:1968366/37‑1 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATg < 1:4758551/37‑1 (MQ=40) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:871361/48‑1 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:4779499/48‑1 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:2006096/1‑68 (MQ=40) acGTCGCAATTCATCTTCACTTCATCCCTGAAATGtt > 1:2476162/1‑37 (MQ=40) acGTCGCAATTCATCTTCACTTCATCCCTGAAATGtt > 1:2047014/1‑37 (MQ=40) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag > 1:3934197/1‑48 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag > 1:530545/1‑48 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGCAATGtt < 1:731112/35‑1 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGAAATGtt < 1:374791/35‑1 (MQ=40) gTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:4869741/38‑1 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:639143/37‑1 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:5482184/37‑1 (MQ=40) cGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGtt > 1:2606835/1‑71 (MQ=38) | ACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTT > minE/822157‑822293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |