| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 822,269 | A→G | 18.7% | intergenic (‑245/+159) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 822,269 | 0 | A | G | 18.7% | 205.8 / 35.1 | 91 | intergenic (‑245/+159) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (37/37); new base G (12/5); total (49/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATT > minE/822200‑822338 | ccGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTa > 1:4998389/1‑70 (MQ=40) cTTGGTGTGGATACTACGTCGCAATTCATCTGCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTc < 1:295500/71‑1 (MQ=255) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:755966/1‑71 (MQ=39) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:4335476/1‑71 (MQ=39) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:4329109/1‑71 (MQ=39) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg < 1:5155005/70‑1 (MQ=40) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg < 1:2021548/70‑1 (MQ=40) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt < 1:4948895/69‑1 (MQ=40) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt < 1:1886170/69‑1 (MQ=40) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt < 1:3742314/69‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGTCTTTTc < 1:3874844/71‑1 (MQ=255) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt < 1:1141021/70‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:1493152/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:3260012/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:2363604/71‑1 (MQ=39) tactacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa < 1:2968349/71‑1 (MQ=39) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2034085/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:360394/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2261381/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2521769/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:3563197/1‑70 (MQ=40) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:2006096/1‑68 (MQ=40) actcAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGt > 1:4185514/4‑71 (MQ=11) cGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGtt > 1:2606835/1‑71 (MQ=38) cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTg < 1:1033075/70‑1 (MQ=35) cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTg > 1:3071989/1‑70 (MQ=35) cAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc < 1:2307039/71‑1 (MQ=34) ttCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt > 1:5149153/1‑52 (MQ=35) ttCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:544924/56‑1 (MQ=35) ttCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:454445/56‑1 (MQ=35) tCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTg > 1:1470259/1‑71 (MQ=35) cATCTTCACTTCATTCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTaaa > 1:493176/1‑56 (MQ=11) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCa > 1:4921806/1‑42 (MQ=255) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCa > 1:4338898/1‑42 (MQ=255) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCa > 1:629988/1‑42 (MQ=255) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTAc > 1:1247510/1‑41 (MQ=38) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTAc > 1:3738006/1‑41 (MQ=38) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTAc > 1:3378411/1‑41 (MQ=38) cATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAAc > 1:2537834/1‑57 (MQ=35) aTCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTc > 1:4798178/1‑44 (MQ=255) aTCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCt > 1:4791674/1‑60 (MQ=11) aTCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGtca < 1:659996/70‑3 (MQ=11) ttcacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCaa > 1:4626209/1‑52 (MQ=11) ttcacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:3095258/1‑52 (MQ=35) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACa > 1:4065603/1‑36 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGttt < 1:2063695/68‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGttt < 1:2890281/68‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTc < 1:1827659/69‑1 (MQ=40) cacttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGt > 1:5077407/1‑71 (MQ=255) acttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg > 1:707559/1‑71 (MQ=255) acttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg > 1:4011666/1‑71 (MQ=255) acttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTg > 1:4098301/1‑71 (MQ=255) cttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCggt < 1:4814080/41‑2 (MQ=255) cttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGttt < 1:4099667/43‑1 (MQ=14) ttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTc < 1:40448/45‑1 (MQ=11) ttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:977655/45‑1 (MQ=38) ttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGc < 1:5588532/51‑1 (MQ=35) ttcaTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGc < 1:2827122/51‑1 (MQ=35) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGTCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAgg > 1:4729365/1‑71 (MQ=255) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt > 1:2050910/1‑42 (MQ=38) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAgg > 1:64072/1‑71 (MQ=255) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAgg > 1:1634326/1‑71 (MQ=255) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAgg > 1:2970997/1‑71 (MQ=255) caTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAg < 1:4052352/70‑1 (MQ=255) aTCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTg < 1:4285390/53‑1 (MQ=35) ccTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCaa > 1:2570016/1‑41 (MQ=14) tGAAATGTTTGCAATGAAGAGTGCATTCCGGtttt < 1:2813744/35‑1 (MQ=14) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTg > 1:2999634/1‑43 (MQ=14) aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTg > 1:5420033/1‑43 (MQ=14) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAg > 1:2517851/1‑62 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa > 1:2602757/1‑71 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa > 1:2646107/1‑71 (MQ=255) aaaTGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTa > 1:3545259/1‑71 (MQ=255) aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAg > 1:303373/1‑39 (MQ=14) gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGc < 1:4691385/68‑1 (MQ=255) gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGc < 1:1179779/68‑1 (MQ=255) gCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGc < 1:560414/68‑1 (MQ=255) cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCg < 1:3172830/70‑1 (MQ=255) cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCg > 1:3446764/1‑70 (MQ=255) cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGc < 1:514652/71‑1 (MQ=255) cAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGc < 1:2235729/71‑1 (MQ=255) aTGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTgg < 1:2068127/58‑1 (MQ=255) tGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGtt > 1:1563116/1‑42 (MQ=255) tGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGc > 1:1322526/1‑48 (MQ=255) tGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAAt > 1:3207426/1‑71 (MQ=255) agagTAGATTCCGGCTTTTCAACAGCTGTTGCAGTGGttt < 1:2549572/40‑1 (MQ=255) gagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCg > 1:4536017/1‑41 (MQ=40) gagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACCGGCTCGCAATTCTg < 1:5105947/71‑1 (MQ=255) gagTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTg < 1:5352516/71‑1 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCaa < 1:5012879/63‑1 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGAtt < 1:438125/71‑1 (MQ=255) tACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGAtt < 1:3305654/71‑1 (MQ=255) | CCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTGGTTTCGTGCAGGGTGTGGTACAGGCTCGCAATTCTGATT > minE/822200‑822338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |