Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,157 C→T 14.7% R205R (CGC→CGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1570CT14.7% 263.9 / 30.3 109R205R (CGC→CGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (52/41);  new base T (6/10);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 113 total aligned reads displayed.

CCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTC  >  minE/832089‑832227
                                                                    |                                                                      
ccGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCt                                                                       >  1:1641594/1‑70 (MQ=255)
     ccGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctct                                                                 >  1:3760901/1‑71 (MQ=255)
      cGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctctc                                                                <  1:432230/71‑1 (MQ=255)
        tgtTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCg                                                               >  1:4606854/1‑70 (MQ=255)
          ttGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCt                                                             >  1:2834931/1‑70 (MQ=255)
          ttGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTg                                                            <  1:4234690/71‑1 (MQ=255)
            gCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGc                                                              >  1:4435639/1‑67 (MQ=255)
              cggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTg                                                        <  1:5681275/70‑1 (MQ=255)
              cggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTg                                                        <  1:4056302/70‑1 (MQ=255)
               ggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTgg                                                       >  1:3516300/1‑71 (MQ=255)
                  ttCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCgg                                                    >  1:309090/1‑71 (MQ=255)
                  ttCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCgg                                                    >  1:797299/1‑71 (MQ=255)
                   tctcGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCgg                                                    >  1:5516512/1‑70 (MQ=255)
                       gCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTgct                                                          >  1:907774/1‑58 (MQ=255)
                       gCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTgct                                                          >  1:3490314/1‑58 (MQ=255)
                       gCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTgct                                                          >  1:5337072/1‑58 (MQ=255)
                       gCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGc                                                                        <  1:4074580/46‑1 (MQ=255)
                         cAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGc                                                                        >  1:2409515/1‑44 (MQ=255)
                          aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTg                                                        <  1:110474/59‑1 (MQ=255)
                          aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTg                                                        <  1:5495926/59‑1 (MQ=255)
                          aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTg                                                        <  1:5503313/59‑1 (MQ=255)
                          aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTg                                                        <  1:1481988/59‑1 (MQ=255)
                           atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                           <  1:921405/68‑1 (MQ=255)
                           aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                <  1:927527/66‑1 (MQ=255)
                           aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:3642011/71‑1 (MQ=255)
                           aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:4752251/71‑1 (MQ=255)
                            ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                          >  1:3654107/1‑55 (MQ=255)
                            ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                          >  1:898989/1‑55 (MQ=255)
                             tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:5452763/69‑1 (MQ=255)
                              ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                        >  1:3502564/1‑71 (MQ=255)
                              ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                        >  1:592971/1‑71 (MQ=255)
                               cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                          <  1:1759196/68‑1 (MQ=255)
                               cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                          <  1:1411874/68‑1 (MQ=255)
                                ggCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                      <  1:5232913/71‑1 (MQ=255)
                                 gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                     >  1:1552504/1‑71 (MQ=255)
                                 gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                     >  1:1360984/1‑71 (MQ=255)
                                  cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGTCGGTACTTGCTTAAGCTTc                                    <  1:5181192/71‑1 (MQ=255)
                                  cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                    <  1:2256318/71‑1 (MQ=255)
                                  cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                    <  1:1379888/71‑1 (MQ=255)
                                    aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGt                                                   <  1:3089293/54‑1 (MQ=255)
                                    aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                >  1:2705791/1‑57 (MQ=255)
                                    aTGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACt                                                >  1:1341708/1‑57 (MQ=255)
                                     tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                           >  1:974481/1‑45 (MQ=255)
                                     tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                 <  1:4317805/71‑1 (MQ=255)
                                       tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                          <  1:1159936/60‑1 (MQ=255)
                                       tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                          <  1:145463/60‑1 (MQ=255)
                                       tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCt                                                             >  1:3910083/1‑41 (MQ=255)
                                       tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                           >  1:5602990/1‑43 (MQ=255)
                                       tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                           >  1:2618927/1‑43 (MQ=255)
                                       tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                               >  1:1965010/1‑71 (MQ=255)
                                       tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                               >  1:4296815/1‑71 (MQ=255)
                                        cGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                           >  1:4349373/1‑42 (MQ=255)
                                        cGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                           >  1:2141681/1‑42 (MQ=255)
                                         gATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                             >  1:2149607/1‑71 (MQ=255)
                                         gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                             >  1:2666366/1‑71 (MQ=255)
                                         gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                             >  1:2233995/1‑71 (MQ=255)
                                           tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                           >  1:1453050/1‑39 (MQ=255)
                                           tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTgg                           <  1:2098656/71‑1 (MQ=255)
                                            ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTgg                           >  1:4249428/1‑70 (MQ=255)
                                             gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGt                          <  1:5306882/70‑1 (MQ=255)
                                             gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                         >  1:1920521/1‑71 (MQ=255)
                                              tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGc                                                      <  1:698589/41‑1 (MQ=255)
                                              tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGc                                                      <  1:2769736/41‑1 (MQ=255)
                                              tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTAc                        >  1:586300/1‑71 (MQ=255)
                                               ttCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                             <  1:1504544/65‑1 (MQ=255)
                                               ttCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                             <  1:966277/65‑1 (MQ=255)
                                               ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:828072/51‑1 (MQ=255)
                                               ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:636632/51‑1 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                 >  1:4721395/1‑44 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                 >  1:2949811/1‑44 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                             >  1:5542322/1‑64 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                       <  1:4581840/70‑1 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                       <  1:428408/70‑1 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                      >  1:3482011/1‑71 (MQ=255)
                                                tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                      >  1:3910588/1‑71 (MQ=255)
                                                  ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                    >  1:291284/1‑55 (MQ=255)
                                                    aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           >  1:4700812/1‑46 (MQ=255)
                                                    aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           >  1:4341842/1‑46 (MQ=255)
                                                    aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                     <  1:4645435/68‑1 (MQ=255)
                                                     cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                 <  1:5182964/55‑1 (MQ=255)
                                                     cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                 <  1:4281585/71‑1 (MQ=255)
                                                      gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                <  1:3784066/71‑1 (MQ=255)
                                                        aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                  >  1:2123407/1‑67 (MQ=255)
                                                        aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                  >  1:3373802/1‑67 (MQ=255)
                                                        aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc              >  1:3964838/1‑71 (MQ=255)
                                                         tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:4734857/41‑1 (MQ=255)
                                                         tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:2032844/41‑1 (MQ=255)
                                                         tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                           <  1:38016/41‑1 (MQ=255)
                                                         tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc             <  1:4910086/71‑1 (MQ=255)
                                                         tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc             <  1:2835482/71‑1 (MQ=255)
                                                         tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc             <  1:121244/71‑1 (MQ=255)
                                                          cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                       <  1:2174563/60‑1 (MQ=255)
                                                           gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc           >  1:4599508/1‑71 (MQ=255)
                                                           gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcct            <  1:4498985/70‑1 (MQ=255)
                                                             gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct         <  1:747071/71‑1 (MQ=255)
                                                               gCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcc          <  1:2590620/68‑1 (MQ=255)
                                                                cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                        >  1:3616390/1‑37 (MQ=255)
                                                                  cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc    <  1:168697/71‑1 (MQ=255)
                                                                   gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                    <  1:2787953/38‑1 (MQ=255)
                                                                    cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc  <  1:1356289/71‑1 (MQ=255)
                                                                    |                                                                      
CCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTC  >  minE/832089‑832227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: