Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,163 C→G 17.5% L207L (CTC→CTG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1630CG17.5% 262.0 / 44.9 114L207L (CTC→CTGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (52/42);  new base G (8/12);  total (60/54)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 133 total aligned reads displayed.

CCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAA  >  minE/832094‑832233
                                                                     |                                                                      
ccGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctct                                                                       >  1:3760901/1‑71 (MQ=255)
 cGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctctc                                                                      <  1:432230/71‑1 (MQ=255)
   tgtTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCg                                                                     >  1:4606854/1‑70 (MQ=255)
     ttGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCt                                                                   >  1:2834931/1‑70 (MQ=255)
     ttGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTg                                                                  <  1:4234690/71‑1 (MQ=255)
       gCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGc                                                                    >  1:4435639/1‑67 (MQ=255)
         cggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTg                                                              <  1:5681275/70‑1 (MQ=255)
         cggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTg                                                              <  1:4056302/70‑1 (MQ=255)
          ggTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTgg                                                             >  1:3516300/1‑71 (MQ=255)
             ttCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCgg                                                          >  1:797299/1‑71 (MQ=255)
             ttCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCgg                                                          >  1:309090/1‑71 (MQ=255)
              tctcGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCgg                                                          >  1:5516512/1‑70 (MQ=255)
                  gCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTgct                                                                >  1:5337072/1‑58 (MQ=255)
                  gCCGATCCCGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTgct                                                                >  1:907774/1‑58 (MQ=255)
                     aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTg                                                              <  1:5503313/59‑1 (MQ=255)
                     aaTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTg                                                              <  1:110474/59‑1 (MQ=255)
                      atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                 <  1:3742798/68‑1 (MQ=255)
                      aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                      <  1:927527/66‑1 (MQ=255)
                      aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:4752251/71‑1 (MQ=255)
                      aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                  >  1:4678871/1‑70 (MQ=255)
                      aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                  >  1:4341106/1‑70 (MQ=255)
                       ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                                >  1:898989/1‑55 (MQ=255)
                       ttCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGAt                                                                >  1:3654107/1‑55 (MQ=255)
                        tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:5452763/69‑1 (MQ=255)
                         ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                 >  1:4061991/1‑52 (MQ=255)
                         ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                              >  1:2197797/1‑71 (MQ=255)
                         ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                              >  1:3502564/1‑71 (MQ=255)
                          cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                <  1:1759196/68‑1 (MQ=255)
                          cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                <  1:1411874/68‑1 (MQ=255)
                           ggCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                            <  1:5232913/71‑1 (MQ=255)
                            gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                           >  1:1552504/1‑71 (MQ=255)
                            gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                           >  1:1360984/1‑71 (MQ=255)
                            gCAATGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                            >  1:3361782/1‑70 (MQ=255)
                             cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                          <  1:1379888/71‑1 (MQ=255)
                             cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                          <  1:2256318/71‑1 (MQ=255)
                               aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGt                                                         <  1:3089293/54‑1 (MQ=255)
                               aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                      >  1:2705791/1‑57 (MQ=255)
                               aTGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACt                                                      >  1:1341708/1‑57 (MQ=255)
                                tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                 >  1:974481/1‑45 (MQ=255)
                                tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                       >  1:1451138/1‑71 (MQ=255)
                                  tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                <  1:1159936/60‑1 (MQ=255)
                                  tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                <  1:145463/60‑1 (MQ=255)
                                  tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCt                                                                   >  1:3910083/1‑41 (MQ=255)
                                  tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                 >  1:5602990/1‑43 (MQ=255)
                                  tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                     >  1:1965010/1‑71 (MQ=255)
                                  tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                     >  1:4296815/1‑71 (MQ=255)
                                   cGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                 >  1:2749841/1‑42 (MQ=255)
                                   cGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                 >  1:2141681/1‑42 (MQ=255)
                                    gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                   >  1:2666366/1‑71 (MQ=255)
                                      tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGa                                                                 >  1:1453050/1‑39 (MQ=255)
                                        gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGt                                <  1:5306882/70‑1 (MQ=255)
                                        gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                               >  1:1920521/1‑71 (MQ=255)
                                        gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                               >  1:1816686/1‑71 (MQ=255)
                                         tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGc                                                            <  1:698589/41‑1 (MQ=255)
                                         tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTAc                              >  1:586300/1‑71 (MQ=255)
                                          ttCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                   <  1:966277/65‑1 (MQ=255)
                                          ttCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                   <  1:1504544/65‑1 (MQ=255)
                                          ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:828072/51‑1 (MQ=255)
                                          ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:636632/51‑1 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                       >  1:4721395/1‑44 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                       >  1:2949811/1‑44 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                   >  1:5542322/1‑64 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                             <  1:428408/70‑1 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                             <  1:4581840/70‑1 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                            >  1:3910588/1‑71 (MQ=255)
                                           tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                            >  1:3482011/1‑71 (MQ=255)
                                               aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 >  1:4341842/1‑46 (MQ=255)
                                               aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                           <  1:4645435/68‑1 (MQ=255)
                                                cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                       <  1:5182964/55‑1 (MQ=255)
                                                cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                       <  1:4281585/71‑1 (MQ=255)
                                                 gcttCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                           <  1:798671/63‑1 (MQ=255)
                                                   aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                        >  1:3373802/1‑67 (MQ=255)
                                                   aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                        >  1:2123407/1‑67 (MQ=255)
                                                    tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:4734857/41‑1 (MQ=255)
                                                    tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:38016/41‑1 (MQ=255)
                                                    tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                 <  1:2032844/41‑1 (MQ=255)
                                                    tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                   <  1:121244/71‑1 (MQ=255)
                                                    tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                   <  1:2835482/71‑1 (MQ=255)
                                                     cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                             <  1:2174563/60‑1 (MQ=255)
                                                      gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcct                  <  1:4498985/70‑1 (MQ=255)
                                                        gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct               <  1:747071/71‑1 (MQ=255)
                                                          gCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcc                <  1:2590620/68‑1 (MQ=255)
                                                           cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                              >  1:3616390/1‑37 (MQ=255)
                                                             cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc          <  1:168697/71‑1 (MQ=255)
                                                              gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                          <  1:2787953/38‑1 (MQ=255)
                                                               ttATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                   <  1:2325871/43‑1 (MQ=12)
                                                                tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTa                                        >  1:5105221/1‑38 (MQ=255)
                                                                tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                      >  1:1171821/1‑56 (MQ=255)
                                                                 aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGAc                        >  1:3205980/1‑53 (MQ=19)
                                                                  tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCg      <  1:3323946/70‑1 (MQ=255)
                                                                  tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc       >  1:5193665/1‑69 (MQ=255)
                                                                   ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                      <  1:3387117/53‑1 (MQ=255)
                                                                   ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                      <  1:1298291/53‑1 (MQ=255)
                                                                   ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc     >  1:5165428/1‑70 (MQ=255)
                                                                    tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                               >  1:2146808/1‑43 (MQ=255)
                                                                    tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                               >  1:2222665/1‑43 (MQ=255)
                                                                    tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct               >  1:1252817/1‑59 (MQ=255)
                                                                    tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct               >  1:687189/1‑59 (MQ=255)
                                                                     ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc       >  1:1526610/1‑66 (MQ=255)
                                                                     ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa  >  1:5052580/1‑71 (MQ=255)
                                                                     |                                                                      
CCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAA  >  minE/832094‑832233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: