Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,178 T→C 15.7% G212G (GGT→GGC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1780TC15.7% 252.8 / 34.3 108G212G (GGT→GGCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (48/43);  new base C (5/12);  total (53/55)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 109 total aligned reads displayed.

ATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAA  >  minE/832116‑832247
                                                              |                                                                     
atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                               <  1:921405/68‑1 (MQ=255)
atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                               <  1:3742798/68‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                                    <  1:927527/66‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:4752251/71‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                >  1:4341106/1‑70 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                >  1:4678871/1‑70 (MQ=255)
  tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:5452763/69‑1 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                            >  1:592971/1‑71 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                            >  1:2197797/1‑71 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                            >  1:3502564/1‑71 (MQ=255)
    cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                              <  1:1759196/68‑1 (MQ=255)
     ggCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                          <  1:5232913/71‑1 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                         >  1:1360984/1‑71 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                         >  1:1552504/1‑71 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                          >  1:3361782/1‑70 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGTCGGTACTTGCTTAAGCTTc                                                        <  1:5181192/71‑1 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                        <  1:1379888/71‑1 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                        <  1:2256318/71‑1 (MQ=255)
         aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGt                                                                       <  1:3089293/54‑1 (MQ=255)
         aTGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACt                                                                    >  1:2705791/1‑57 (MQ=255)
         aTGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACt                                                                    >  1:1341708/1‑57 (MQ=255)
          tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                     >  1:1451138/1‑71 (MQ=255)
          tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                     <  1:4317805/71‑1 (MQ=255)
            tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                              <  1:1159936/60‑1 (MQ=255)
            tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                              <  1:145463/60‑1 (MQ=255)
            tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                   >  1:4296815/1‑71 (MQ=255)
            tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                   >  1:1965010/1‑71 (MQ=255)
              gATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                 >  1:2149607/1‑71 (MQ=255)
              gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                 >  1:2666366/1‑71 (MQ=255)
              gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                 >  1:2233995/1‑71 (MQ=255)
                tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTgg                                               <  1:2098656/71‑1 (MQ=255)
                 ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTgg                                               >  1:4249428/1‑70 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGt                                              <  1:5306882/70‑1 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                             >  1:1816686/1‑71 (MQ=255)
                    ttCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                 <  1:1504544/65‑1 (MQ=255)
                    ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:636632/51‑1 (MQ=255)
                    ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:828072/51‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                                     >  1:4721395/1‑44 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTAc                                                                     >  1:2949811/1‑44 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                 >  1:5542322/1‑64 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                           <  1:4581840/70‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                           <  1:428408/70‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                          >  1:3482011/1‑71 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                          >  1:3910588/1‑71 (MQ=255)
                       ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                        >  1:291284/1‑55 (MQ=255)
                         aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               >  1:4700812/1‑46 (MQ=255)
                         aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                         <  1:4645435/68‑1 (MQ=255)
                          cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                     <  1:5182964/55‑1 (MQ=255)
                          cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                     <  1:4281585/71‑1 (MQ=255)
                           gcttCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                         <  1:798671/63‑1 (MQ=255)
                           gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                    <  1:3784066/71‑1 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                      >  1:2123407/1‑67 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                      >  1:3373802/1‑67 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc                                  >  1:3964838/1‑71 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:4734857/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:2032844/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                               <  1:38016/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                 <  1:4910086/71‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                 <  1:2835482/71‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                 <  1:121244/71‑1 (MQ=255)
                               cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                           <  1:2174563/60‑1 (MQ=255)
                                gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                               >  1:4599508/1‑71 (MQ=255)
                                gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcct                                <  1:4498985/70‑1 (MQ=255)
                                  gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                             <  1:747071/71‑1 (MQ=255)
                                    gCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcc                              <  1:2590620/68‑1 (MQ=255)
                                     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                            >  1:3616390/1‑37 (MQ=255)
                                       cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                        <  1:168697/71‑1 (MQ=255)
                                        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                        <  1:2787953/38‑1 (MQ=255)
                                         ttATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                 <  1:3373230/43‑1 (MQ=12)
                                         cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc                      <  1:1356289/71‑1 (MQ=255)
                                          tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                    >  1:1171821/1‑56 (MQ=255)
                                           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGAc                                      >  1:3175116/1‑53 (MQ=19)
                                           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGAc                                      >  1:3205980/1‑53 (MQ=19)
                                           aTCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                        <  1:1875241/35‑1 (MQ=255)
                                            tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                     >  1:5193665/1‑69 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                    <  1:5300695/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                    <  1:3387117/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                    <  1:1298291/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                   >  1:5165428/1‑70 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                   >  1:1631215/1‑37 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                             >  1:1441997/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                             >  1:2222665/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                             >  1:802918/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                             >  1:2146808/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                             >  1:1252817/1‑59 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                             >  1:687189/1‑59 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                             >  1:725684/1‑59 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                     >  1:1526610/1‑66 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                     >  1:1235824/1‑66 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                >  1:5052580/1‑71 (MQ=255)
                                                tcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgatg                 <  1:761100/69‑4 (MQ=255)
                                                tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc               >  1:3511473/1‑71 (MQ=255)
                                                  gctgctTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTg                                   >  1:3583570/1‑49 (MQ=16)
                                                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc              <  1:569964/70‑1 (MQ=255)
                                                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc              <  1:2597733/70‑1 (MQ=255)
                                                     gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc              <  1:3422109/67‑1 (MQ=255)
                                                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg          >  1:5620596/1‑69 (MQ=255)
                                                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc        <  1:1200017/71‑1 (MQ=255)
                                                         ggCGGTACATGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg       <  1:1009608/70‑1 (MQ=255)
                                                             gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCaa  <  1:3585810/71‑1 (MQ=255)
                                                              |                                                                     
ATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAA  >  minE/832116‑832247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: