Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 832,184 C→T 13.5% C214C (TGC→TGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE832,1840CT13.5% 283.8 / 27.5 111C214C (TGC→TGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (50/46);  new base T (5/10);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 114 total aligned reads displayed.

ATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACG  >  minE/832116‑832254
                                                                    |                                                                      
atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                                      <  1:921405/68‑1 (MQ=255)
atcccGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGttt                                                                      <  1:3742798/68‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:4752251/71‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:3642011/71‑1 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                       >  1:4678871/1‑70 (MQ=255)
aTTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCt                                                                       >  1:4341106/1‑70 (MQ=255)
  tCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:5452763/69‑1 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                                   >  1:592971/1‑71 (MQ=255)
   ccGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                                   >  1:2197797/1‑71 (MQ=255)
    cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                     <  1:1759196/68‑1 (MQ=255)
    cGGCGATGTCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                     <  1:1411874/68‑1 (MQ=255)
     ggCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                                 <  1:5232913/71‑1 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                                >  1:1360984/1‑71 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCtt                                                                >  1:1552504/1‑71 (MQ=255)
      gCAATGTCGATGGTTTCTTACGCATCGGGTGAACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCt                                                                 >  1:3361782/1‑70 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                               <  1:1379888/71‑1 (MQ=255)
       cAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                               <  1:2256318/71‑1 (MQ=255)
          tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                            <  1:4317805/71‑1 (MQ=255)
          tGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                            >  1:1451138/1‑71 (MQ=255)
            tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                     <  1:1159936/60‑1 (MQ=255)
            tCGATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTa                                                                     <  1:145463/60‑1 (MQ=255)
            tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                          >  1:4296815/1‑71 (MQ=255)
            tCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                          >  1:1965010/1‑71 (MQ=255)
              gATGGTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                        >  1:2149607/1‑71 (MQ=255)
              gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                        >  1:2666366/1‑71 (MQ=255)
              gATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                        >  1:2233995/1‑71 (MQ=255)
                tGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTgg                                                      <  1:2098656/71‑1 (MQ=255)
                 ggTTTCTTACGCCGCCGGAACGCGTTATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTgg                                                      >  1:4249428/1‑70 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGt                                                     <  1:5306882/70‑1 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    >  1:1816686/1‑71 (MQ=255)
                  gTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    >  1:1920521/1‑71 (MQ=255)
                   tttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTAc                                                   >  1:586300/1‑71 (MQ=255)
                    ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:828072/51‑1 (MQ=255)
                    ttCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:636632/51‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACt                                                        >  1:5542322/1‑64 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                                  <  1:4581840/70‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                                  <  1:428408/70‑1 (MQ=255)
                     tCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTg                                                 >  1:3482011/1‑71 (MQ=255)
                       ttACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                               >  1:291284/1‑55 (MQ=255)
                         aCGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                                <  1:4645435/68‑1 (MQ=255)
                          cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTAc                                                            <  1:5182964/55‑1 (MQ=255)
                          cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt                                            <  1:4281585/71‑1 (MQ=255)
                           gcttCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGc                                                <  1:798671/63‑1 (MQ=255)
                           gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           <  1:3784066/71‑1 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGAc                                             >  1:3373802/1‑67 (MQ=255)
                             aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc                                         >  1:3964838/1‑71 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:4734857/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:38016/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCtt                                                                      <  1:2032844/41‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                        <  1:2835482/71‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                        <  1:4910086/71‑1 (MQ=255)
                              tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc                                        <  1:121244/71‑1 (MQ=255)
                               cGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACt                                                  <  1:2174563/60‑1 (MQ=255)
                                gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                      >  1:4599508/1‑71 (MQ=255)
                                gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcct                                       <  1:4498985/70‑1 (MQ=255)
                                  gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                    <  1:747071/71‑1 (MQ=255)
                                    gCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcc                                     <  1:2590620/68‑1 (MQ=255)
                                     cACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAg                                                                   >  1:3616390/1‑37 (MQ=255)
                                       cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc                               <  1:168697/71‑1 (MQ=255)
                                        gCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                               <  1:2787953/38‑1 (MQ=255)
                                         ttATCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACt                                                        <  1:3373230/43‑1 (MQ=12)
                                          tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTa                                                             >  1:5105221/1‑38 (MQ=255)
                                          tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           >  1:1171821/1‑56 (MQ=255)
                                           aTCTGTCGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGAc                                             >  1:3175116/1‑53 (MQ=19)
                                           aTCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTc                                                               <  1:1875241/35‑1 (MQ=255)
                                            tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCg                           <  1:3323946/70‑1 (MQ=255)
                                            tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                            >  1:5193665/1‑69 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           <  1:1298291/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           <  1:5300695/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt                                           <  1:3387117/53‑1 (MQ=255)
                                             ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc                          >  1:5165428/1‑70 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGa                                                          >  1:1631215/1‑37 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    >  1:2146808/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    >  1:1441997/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    >  1:2222665/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTa                                                    >  1:802918/1‑43 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                    >  1:725684/1‑59 (MQ=255)
                                              tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                    >  1:1252817/1‑59 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                            >  1:1526610/1‑66 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                            >  1:1235824/1‑66 (MQ=255)
                                               ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa                       >  1:5052580/1‑71 (MQ=255)
                                                tcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgatg                        <  1:761100/69‑4 (MQ=255)
                                                tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc                      >  1:3511473/1‑71 (MQ=255)
                                                  gctgctTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTg                                          >  1:3583570/1‑49 (MQ=16)
                                                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc                     <  1:2597733/70‑1 (MQ=255)
                                                  gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc                     <  1:569964/70‑1 (MQ=255)
                                                     gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc                     <  1:3422109/67‑1 (MQ=255)
                                                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg                 >  1:5620596/1‑69 (MQ=255)
                                                       ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc               <  1:1200017/71‑1 (MQ=255)
                                                         ggCGGTACATGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg              <  1:1009608/70‑1 (MQ=255)
                                                             gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCaa         <  1:3585810/71‑1 (MQ=255)
                                                               aCTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa          <  1:663507/68‑1 (MQ=255)
                                                                cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAAc       <  1:4644879/70‑1 (MQ=255)
                                                                cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt      <  1:163062/71‑1 (MQ=255)
                                                                 ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                    >  1:4451249/1‑40 (MQ=255)
                                                                 ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct                                    >  1:1414845/1‑40 (MQ=255)
                                                                  tGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc                                      >  1:631224/1‑37 (MQ=255)
                                                                  tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                            >  1:4719233/1‑47 (MQ=255)
                                                                  tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc                            >  1:1707352/1‑47 (MQ=255)
                                                                    cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg  <  1:1722190/71‑1 (MQ=255)
                                                                    |                                                                      
ATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACG  >  minE/832116‑832254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: