| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 832,212 | T→C | 20.2% | L224L (TTG→CTG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 832,212 | 0 | T | C | 20.2% | 160.4 / 34.0 | 82 | L224L (TTG→CTG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (33/32); new base C (6/10); total (40/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
CGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTC > minE/832142‑832282 | cGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACt < 1:4281585/71‑1 (MQ=255) gCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt < 1:3784066/71‑1 (MQ=255) aTCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGc > 1:3964838/1‑71 (MQ=255) tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc < 1:4910086/71‑1 (MQ=255) tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc < 1:2835482/71‑1 (MQ=255) tCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcc < 1:121244/71‑1 (MQ=255) gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc > 1:4599508/1‑71 (MQ=255) gggTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcct < 1:4498985/70‑1 (MQ=255) gTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct < 1:747071/71‑1 (MQ=255) gCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcc < 1:2590620/68‑1 (MQ=255) cGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtc < 1:168697/71‑1 (MQ=255) cTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGtctc < 1:1356289/71‑1 (MQ=255) tATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt > 1:1171821/1‑56 (MQ=255) tctgtcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCg < 1:3323946/70‑1 (MQ=255) tctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:5193665/1‑69 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt < 1:3387117/53‑1 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt < 1:1298291/53‑1 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACtt < 1:5300695/53‑1 (MQ=255) ctctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc > 1:5165428/1‑70 (MQ=255) tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct > 1:725684/1‑59 (MQ=255) tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct > 1:1252817/1‑59 (MQ=255) tctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct > 1:687189/1‑59 (MQ=255) ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:1235824/1‑66 (MQ=255) ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:1526610/1‑66 (MQ=255) ctcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaaa > 1:5052580/1‑71 (MQ=255) tcGCTGCTTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCgatg < 1:761100/69‑4 (MQ=255) tcGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAc > 1:3511473/1‑71 (MQ=255) gctgctTGGCGGCACCTGTTTAAGTTTCTACGACTGGTATTGCGACCTg > 1:3583570/1‑49 (MQ=16) gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc < 1:569964/70‑1 (MQ=255) gCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc < 1:2597733/70‑1 (MQ=255) gATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAAcc < 1:3422109/67‑1 (MQ=255) ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTggg > 1:5620596/1‑69 (MQ=255) ttGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGc < 1:1200017/71‑1 (MQ=255) ggCGGTACATGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCg < 1:1009608/70‑1 (MQ=255) gTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCaa < 1:3585810/71‑1 (MQ=255) aCTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa < 1:663507/68‑1 (MQ=255) cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAAc < 1:4644879/70‑1 (MQ=255) cTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACt < 1:163062/71‑1 (MQ=255) ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct > 1:1414845/1‑40 (MQ=255) ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctcct > 1:4451249/1‑40 (MQ=255) ttGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCaa < 1:5615593/52‑1 (MQ=255) tGCTTAAGCTTCTACGACTGGTACTGCGACTTGcctc > 1:631224/1‑37 (MQ=255) tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:4719233/1‑47 (MQ=255) tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:1723349/1‑47 (MQ=255) tGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTcc > 1:1707352/1‑47 (MQ=255) cTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACg < 1:1722190/71‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGt < 1:3382378/70‑1 (MQ=255) tAAGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGt < 1:3375919/70‑1 (MQ=255) tAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGa > 1:2239762/1‑58 (MQ=255) tAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGa > 1:5256285/1‑58 (MQ=255) aaGTTTCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTa < 1:1006427/70‑1 (MQ=255) aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGc > 1:3515463/1‑44 (MQ=255) aaGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGt < 1:4166571/69‑1 (MQ=255) tttCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGc < 1:3806603/51‑1 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGaa > 1:1655704/1‑71 (MQ=255) tCTACGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGa < 1:3303868/70‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:999977/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGa < 1:2123804/69‑1 (MQ=255) cTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:3042141/1‑71 (MQ=255) tACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:1807426/1‑70 (MQ=255) tACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATc > 1:1396551/1‑71 (MQ=255) tACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATc > 1:1283628/1‑71 (MQ=255) aCGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt > 1:4469270/1‑69 (MQ=255) cGACTGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATc < 1:5684819/69‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAAt < 1:90272/68‑1 (MQ=255) cGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATct > 1:4196601/1‑70 (MQ=255) cTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATctgctg < 1:1065204/71‑1 (MQ=255) tGGTATTGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCgg < 1:1009180/61‑1 (MQ=255) ggTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGAc > 1:5140846/1‑71 (MQ=255) ttGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGaa < 1:2171945/57‑1 (MQ=255) ttGCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGAc > 1:1859496/2‑67 (MQ=255) cTGCGTCTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACt < 1:1194552/68‑1 (MQ=255) cTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGa > 1:3899584/1‑39 (MQ=255) cTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa < 1:2842309/42‑1 (MQ=255) cTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCa < 1:3609614/42‑1 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa > 1:3097322/1‑70 (MQ=255) gCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTa > 1:180735/1‑70 (MQ=255) gCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGa > 1:3131241/1‑46 (MQ=255) gCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAAt > 1:5092699/1‑57 (MQ=255) gCGACCTGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAAt > 1:3352922/1‑57 (MQ=255) gCGACATGCCGCCCGCCTCGCCGATGACCTGGGGCGAGCAAACCGACGTACCGGAATCTGCCGACTGGTa > 1:2420630/1‑70 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTg > 1:2286052/1‑66 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:1238824/70‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTAc < 1:3566441/70‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa < 1:4997379/71‑1 (MQ=255) cGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACa < 1:945171/71‑1 (MQ=255) gACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACaa < 1:3450789/71‑1 (MQ=255) aCTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAAc > 1:3771725/1‑71 (MQ=255) ttGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTc > 1:418955/1‑71 (MQ=255) | CGCATCGGGTGCACGCTATCTCTCGCTGATTGGCGGTACTTGCTTAAGCTTCTACGACTGGTACTGCGACTTGCCTCCTGCGTCTCCGCAAACCTGGGGCGAGCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTC > minE/832142‑832282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |